Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human  Serum Albumin

M. M. Abu TEIR; J. GHITHAN; S. DARWISH; M. M. ABU-HADID

Makale detayları

Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

Yazarlar:

M. M. Abu TEIR | J. GHITHAN | S. DARWISH | M. M. ABU-HADID

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2012 Vol.6 Issue 3, pp.45-55

Belge Tipi:

Makale

Yayın Tarihi:

10/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000831474

Sıklık:

Üç Yıllık

eISSN:

2146-0108

Özet:

 

The interaction between cholesterol and human serum albumin has been investigated. The basic binding interaction was studied by UVabsorption and fluorescence spectroscopy. From spectral analysis cholesterol showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching mechanism. The binding constant (k) is estimated to be K=0.214 × 104 M-1 at 293 K. FTIR spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and cholesterol binding mechanisms. The observed spectral changes indicate a higher percentage of H-bonding between cholesterol and a-helix (secondary structure motif in HSA) compared to the percentage of H-bonding between cholesterol and b-sheets (secondary structure motif in HSA).

Makale detayları

Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

Yazarlar: M. M. Abu TEIR | J. GHITHAN | S. DARWISH | M. M. ABU-HADID

Yayıncı: Nobel Bilim ve Araştırma Merkezi Limited

Dergi: Journal of Applied Biological Sciences 2012 Vol.6 Issue 3, pp.45-55

Belge Tipi: Makale

Yayın Tarihi: 10/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000831474

Sıklık: Üç Yıllık

eISSN: 2146-0108

Sayı

Yazarlar:

M. M. Abu TEIR | J. GHITHAN | S. DARWISH | M. M. ABU-HADID

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2012 Vol.6 Issue 3, pp.45-55

Belge Tipi:

Makale

Yayın Tarihi:

10/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000831474

Sıklık:

Üç Yıllık

eISSN:

2146-0108