Investigation of Possible Protective Effects of Resveratrol on Oxidative Stress and Ferroptosis in PFOA Exposure in HepG-2 Cells

Didem Oral; Ceyhan Hacıoğlu

Makale detayları

Investigation of Possible Protective Effects of Resveratrol on Oxidative Stress and Ferroptosis in PFOA Exposure in HepG-2 Cells

Yazarlar:

Didem Oral | Ceyhan Hacıoğlu

Yayıncı:

Duzce University

Ana Başlıklar:

Health Services and Systems (Other)

Dergi:

Konuralp Medical Journal Vol.16 Issue 3, pp.283-292

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330621

pISSN:

1309-3878

Anahtar kelimeler:

Özet:

 Objective: Exposure to perfluorooctanoic acid PFOA is linked to adverse health effects, including cancer and hepatic diseases. PFOA induces reactive oxygen species generation in human hepatic cells, causing oxidative stress and cell death. Resveratrol RSV has garnered attention for its protective effects against xenobiotic-induced damage, yet its impact on PFOA-induced oxidative stress and ferroptosis in the liver remains understudied. This study investigates RSVs protective mechanisms against oxidative stress and ferroptosis in HepG2 cells exposed to PFOA. Method: HepG2 cells were cultured in DMEM with 10 FBS and 1 penicillinstreptomycin in a 5 CO2 incubator at 37C. PFOA was added to the cells at concentrations ranging from 0 to 450 µM and incubated at 37C for 24 hours. The IC50 was determined to be 457 μM. To examine RSVs protective effects, cells were treated with 60 μM RSV. Following treatment with PFOA, RSV, and the combination of PFOARSV, cell lysates were prepared for analysis. Oxidative stress and ferroptosis parameters were measured spectrophotometrically using ELISA. Results: In the PFOARSV group, antioxidant capacity increased, and ferroptosis was suppressed compared to the control. Conversely, the PFOA group showed decreased antioxidant capacity, increased oxidant capacity, and induced ferroptosis compared to the control and RSV-treated groups. Conclusion: PFOA exposure heightens oxidative stress and ferroptosis, whereas RSV treatment significantly reduces hepatic oxidative stress and protects against ferroptosis during PFOA exposure.

 Objective: Exposure to perfluorooctanoic acid PFOA is linked to adverse health effects, including cancer and hepatic diseases. PFOA induces reactive oxygen species generation in human hepatic cells, causing oxidative stress and cell death. Resveratrol RSV has garnered attention for its protective effects against xenobiotic-induced damage, yet its impact on PFOA-induced oxidative stress and ferroptosis in the liver remains understudied. This study investigates RSVs protective mechanisms against oxidative stress and ferroptosis in HepG2 cells exposed to PFOA. Method: HepG2 cells were cultured in DMEM with 10 FBS and 1 penicillinstreptomycin in a 5 CO2 incubator at 37C. PFOA was added to the cells at concentrations ranging from 0 to 450 µM and incubated at 37C for 24 hours. The IC50 was determined to be 457 μM. To examine RSVs protective effects, cells were treated with 60 μM RSV. Following treatment with PFOA, RSV, and the combination of PFOARSV, cell lysates were prepared for analysis. Oxidative stress and ferroptosis parameters were measured spectrophotometrically using ELISA. Results: In the PFOARSV group, antioxidant capacity increased, and ferroptosis was suppressed compared to the control. Conversely, the PFOA group showed decreased antioxidant capacity, increased oxidant capacity, and induced ferroptosis compared to the control and RSV-treated groups. Conclusion: PFOA exposure heightens oxidative stress and ferroptosis, whereas RSV treatment significantly reduces hepatic oxidative stress and protects against ferroptosis during PFOA exposure.

 Objective: Exposure to perfluorooctanoic acid PFOA is linked to adverse health effects, including cancer and hepatic diseases. PFOA induces reactive oxygen species generation in human hepatic cells, causing oxidative stress and cell death. Resveratrol RSV has garnered attention for its protective effects against xenobiotic-induced damage, yet its impact on PFOA-induced oxidative stress and ferroptosis in the liver remains understudied. This study investigates RSVs protective mechanisms against oxidative stress and ferroptosis in HepG2 cells exposed to PFOA. Method: HepG2 cells were cultured in DMEM with 10 FBS and 1 penicillinstreptomycin in a 5 CO2 incubator at 37C. PFOA was added to the cells at concentrations ranging from 0 to 450 µM and incubated at 37C for 24 hours. The IC50 was determined to be 457 μM. To examine RSVs protective effects, cells were treated with 60 μM RSV. Following treatment with PFOA, RSV, and the combination of PFOARSV, cell lysates were prepared for analysis. Oxidative stress and ferroptosis parameters were measured spectrophotometrically using ELISA. Results: In the PFOARSV group, antioxidant capacity increased, and ferroptosis was suppressed compared to the control. Conversely, the PFOA group showed decreased antioxidant capacity, increased oxidant capacity, and induced ferroptosis compared to the control and RSV-treated groups. Conclusion: PFOA exposure heightens oxidative stress and ferroptosis, whereas RSV treatment significantly reduces hepatic oxidative stress and protects against ferroptosis during PFOA exposure.

 Amaç: Perflorooktanoik asit PFOA maruziyeti, kanser ve karaciğer hastalıkları dahil olmak üzere çeşitli olumsuz sağlık etkileri ile ilişkilendirilmiştir. PFOA, insan karaciğer hücrelerinde reaktif oksijen türlerinin oluşumunu indükleyerek oksidatif strese ve hücre ölümüne neden olur. Son yıllarda, Resveratrolün RSV ksenobiyotiklerin neden olduğu hasarlara karşı koruyucu etkisi üzerine çalışmalar önem kazanmıştır. Ancak, RSVnin karaciğerde PFOAnın neden olduğu oksidatif stres ve ferroptozis üzerindeki koruyucu etkileri hakkında yeterli çalışma bulunmamaktadır. Bu çalışmada, PFOA maruziyeti sonucu HepG2 hücrelerinde oluşabilecek oksidatif stres ve ferroptozis üzerine RSVnin koruyucu etki mekanizmalarını araştırmayı amaçladık. Metod: HepG2 hücreleri, 5 CO2 inkübatöründe 37Cde 10 FBS ve 1 penisilinstreptomisin içeren DMEMde kültürlendi. PFOA, 0-450 µM konsantrasyonlarında hücrelere eklendi ve 37Cde 24 saat inkübe edildi. IC50, 457 μM olarak belirlendi. RSVnin koruyucu etkilerini değerlendirmek için hücreler 60 μM RSV ile muamele edildi. PFOA, RSV ve PFOARSV ile muamele sonrasında, hücre lizatı hazırlandı ve analizler için kullanıldı. Oksidatif stres ve ferroptozis parametreleri, ELISA yöntemiyle spektrofotometrik olarak belirlendi. Bulgular: PFOARSV grubunda, kontrol grubuna kıyasla antioksidan kapasite artmış ve ferroptozis baskılanmıştır. Buna karşılık, PFOA grubunda antioksidan kapasite azalmış, oksidan kapasite artmış ve kontrol ve RSV ile muamele edilen gruplara kıyasla ferroptozis indüklenmiştir. Sonuç: PFOA maruziyeti oksidatif stresi ve ferroptozisi artırırken, RSV tedavisi karaciğer oksidatif stresini önemli ölçüde azaltır ve PFOA maruziyeti sırasında ferroptozise karşı korur.

Makale detayları

Investigation of Possible Protective Effects of Resveratrol on Oxidative Stress and Ferroptosis in PFOA Exposure in HepG-2 Cells

Yazarlar:

Didem Oral | Ceyhan Hacıoğlu

Yayıncı:

Duzce University

Ana Başlıklar:

Health Services and Systems (Other)

Dergi:

Konuralp Medical Journal Vol.16 Issue 3, pp.283-292

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330621

pISSN:

1309-3878

PFOA

Resveratrol

Oxidative stress

HepG2

FerroptosisPFOA

Resveratrol

Oksidatif Stres

HepG2

Ferroptoz

Sayı

Makale detayları

Yazarlar: Didem Oral | Ceyhan Hacıoğlu

Yayıncı: Duzce University

Ana Başlıklar: Health Services and Systems (Other)

Dergi: Konuralp Medical Journal Vol.16 Issue 3, pp.283-292

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001330621

pISSN: 1309-3878

Sayı

Yazarlar:

Didem Oral | Ceyhan Hacıoğlu

Yayıncı:

Duzce University

Ana Başlıklar:

Health Services and Systems (Other)

Dergi:

Konuralp Medical Journal Vol.16 Issue 3, pp.283-292

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330621

pISSN:

1309-3878