Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Murat ŞEVİK; Fahri Ferudun ÖZTÜRK

Makale detayları

Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Yazarlar:

Murat ŞEVİK | Fahri Ferudun ÖZTÜRK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.523-528

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766278

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

In this study, we compared 2 methods, liquid-phase blocking ELISA (LPBE) and solid-phase competition ELISA (SPCE), for the detection of antibodies to the structural proteins of foot-and-mouth disease virus (FMDV). These methods were compared using sera collected from cattle (n = 30) without a history of foot-and-mouth disease infection or vaccination, cattle (n = 180) vaccinated with oil-adjuvanted bivalent vaccine, and international reference sera (positive, weak positive, and negative) for FMDV serotypes O and A. The results showed that SPCE had a better specificity (96.67% for serotype O and 100% for serotype A) than LPBE (90% for serotype O and 93.33% for serotype A). Sensitivity of LPBE (97.22% for serotype O and 98.33% for serotype A) was almost equivalent to that of SPCE (98.33% for serotype O and 98.89% for serotype A). It can be concluded that SPCE is more suitable than LPBE for use as a screening test for the detection of antibodies against structural proteins of FMDV.

 

In this study, we compared 2 methods, liquid-phase blocking ELISA (LPBE) and solid-phase competition ELISA (SPCE), for the detection of antibodies to the structural proteins of foot-and-mouth disease virus (FMDV). These methods were compared using sera collected from cattle (n = 30) without a history of foot-and-mouth disease infection or vaccination, cattle (n = 180) vaccinated with oil-adjuvanted bivalent vaccine, and international reference sera (positive, weak positive, and negative) for FMDV serotypes O and A. The results showed that SPCE had a better specificity (96.67% for serotype O and 100% for serotype A) than LPBE (90% for serotype O and 93.33% for serotype A). Sensitivity of LPBE (97.22% for serotype O and 98.33% for serotype A) was almost equivalent to that of SPCE (98.33% for serotype O and 98.89% for serotype A). It can be concluded that SPCE is more suitable than LPBE for use as a screening test for the detection of antibodies against structural proteins of FMDV.

Makale detayları

Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Yazarlar:

Murat ŞEVİK | Fahri Ferudun ÖZTÜRK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.523-528

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766278

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Cattle

foot-and-mouth disease

structural protein

liquid-phase blocking ELISA

solid-phase competition ELISA

Sayı

Makale detayları

Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Yazarlar: Murat ŞEVİK | Fahri Ferudun ÖZTÜRK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.523-528

Belge Tipi: Makale

Yayın Tarihi: 4/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000766278

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Cattle

foot-and-mouth disease

structural protein

liquid-phase blocking ELISA

solid-phase competition ELISA

Sayı

Yazarlar:

Murat ŞEVİK | Fahri Ferudun ÖZTÜRK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 5, pp.523-528

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766278

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128