Machilus thunbergii extract inhibits inflammatory response inlipopolysaccharide-induced RAW264.7 murine macrophages viasuppression of NF-κκ\kappaB and p38 MAPK activation

SHU-JU WU; WEN-BIN LEN; Chien-yi HUANG; Chian-jiun LIOU; WEN-CHUNG HUANG; Chwan-fwu LIN

Makale detayları

Machilus thunbergii extract inhibits inflammatory response inlipopolysaccharide-induced RAW264.7 murine macrophages viasuppression of NF-κκ\kappaB and p38 MAPK activation

Yazarlar:

SHU-JU WU | WEN-BIN LEN | Chien-yi HUANG | Chian-jiun LIOU | WEN-CHUNG HUANG | Chwan-fwu LIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.657-665

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811831

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Machilus thunbergii Sieb. and Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW 264.7 cells. The cells were pretreated with various doses (12.5 μg/mL-100 μg/mL) of M. thunbergii extract and activated with LPS. We found that 25 μg/mL M. thunbergii extract inhibited nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) expression, as well as levels of proinflammatory cytokines including interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). Furthermore, M. thunbergii extract also suppressed translocation of nuclear transcription factor kappa-B (NF-?B) subunit p65 proteins into the nucleus and induced a dose-dependent inhibition of the phosphorylation of p38 mitogen-activated protein kinase (MAPK). M. thunbergii extract also increased HO-1 and Nrf2 production in a concentration-dependent manner. Taken together, these results suggest that M. thunbergii extract has an anti-inflammatory effect because it reduces levels of proinflammatory cytokines and mediators via the suppression of NF-?B and p38 MAPK activation in RAW 264.7 cells.

 

Machilus thunbergii Sieb. and Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW 264.7 cells. The cells were pretreated with various doses (12.5 μg/mL-100 μg/mL) of M. thunbergii extract and activated with LPS. We found that 25 μg/mL M. thunbergii extract inhibited nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) expression, as well as levels of proinflammatory cytokines including interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). Furthermore, M. thunbergii extract also suppressed translocation of nuclear transcription factor kappa-B (NF-?B) subunit p65 proteins into the nucleus and induced a dose-dependent inhibition of the phosphorylation of p38 mitogen-activated protein kinase (MAPK). M. thunbergii extract also increased HO-1 and Nrf2 production in a concentration-dependent manner. Taken together, these results suggest that M. thunbergii extract has an anti-inflammatory effect because it reduces levels of proinflammatory cytokines and mediators via the suppression of NF-?B and p38 MAPK activation in RAW 264.7 cells.

Makale detayları

Machilus thunbergii extract inhibits inflammatory response inlipopolysaccharide-induced RAW264.7 murine macrophages viasuppression of NF-κκ\kappaB and p38 MAPK activation

Yazarlar:

SHU-JU WU | WEN-BIN LEN | Chien-yi HUANG | Chian-jiun LIOU | WEN-CHUNG HUANG | Chwan-fwu LIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.657-665

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811831

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Cyclooxygenase-2

inducible nitric oxide synthase

Machilus thunbergii

NF-κκ\kappaB

p38 MAPK

Sayı

Makale detayları

Machilus thunbergii extract inhibits inflammatory response inlipopolysaccharide-induced RAW264.7 murine macrophages viasuppression of NF-κκ\kappaB and p38 MAPK activation

Yazarlar: SHU-JU WU | WEN-BIN LEN | Chien-yi HUANG | Chian-jiun LIOU | WEN-CHUNG HUANG | Chwan-fwu LIN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.657-665

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811831

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Cyclooxygenase-2

inducible nitric oxide synthase

Machilus thunbergii

NF-κκ\kappaB

p38 MAPK

Sayı

Yazarlar:

SHU-JU WU | WEN-BIN LEN | Chien-yi HUANG | Chian-jiun LIOU | WEN-CHUNG HUANG | Chwan-fwu LIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.657-665

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811831

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152