Characterization of the promoter region of the glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum

LIJING CHEN; LI ZHANG; WEIKANG QI; MUHAMMAD IRFAN; JING-WEI LIN; HUI MA; ZHI-FU GUO; MING ZHONG; Tian-lai LI

Makale detayları

Characterization of the promoter region of the glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum

Yazarlar:

LIJING CHEN | LI ZHANG | WEIKANG QI | MUHAMMAD IRFAN | JING-WEI LIN | HUI MA | ZHI-FU GUO | MING ZHONG | Tian-lai LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 3, pp.552-562

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811407

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Cold environmental conditions influence the growth and development of plants, causing crop reduction or even plant death. Under stress conditions, cold-inducible promoters regulate cold-related gene expression as a molecular switch. Recent studies have shown that the chloroplast-expressed GPAT gene plays an important role in determining cold sensitivity. However, the mechanism of the transcriptional regulation of GPAT is ambiguous. The 5'-flanking region of GPAT with length of 1494 bp was successfully obtained by chromosome walking from Lilium pensylvanicum. The cis-elements of GPAT promoters were predicted and analyzed by a plant cis-acting regulatory DNA element database. There exist core promoter regions including TATA-box and CAAT-box and transcription regulation regions, which involve some regulatory elements such as I-box, W-box, MYB, MYC, and DREB. Full-length and four 5'-deletion fragments linked with GUS vectors were constructed and transformed into Nicotiana tabacum by Agrobacterium-mediated transformation. Transient transformation and histochemical staining of leaves indicated that the activity of the GPAT promoter was strong and induced by low-temperature stress. The deletion of a -294 bp region suggested that the DRE-motif was functionally an essential element for cold induction, and the deletion of -1494 bp and -1194 bp regions suggested that negative regulation exists in the promoter. Our results show that the GPAT gene promoter is a key regulator under cold stress and we think that this study will have significant impact on lily molecular breeding and improving the resistance of plants.

Makale detayları

Characterization of the promoter region of the glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum

Yazarlar: LIJING CHEN | LI ZHANG | WEIKANG QI | MUHAMMAD IRFAN | JING-WEI LIN | HUI MA | ZHI-FU GUO | MING ZHONG | Tian-lai LI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2017 Vol.41 Issue 3, pp.552-562

Belge Tipi: Makale

Yayın Tarihi: 5/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000811407

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

LIJING CHEN | LI ZHANG | WEIKANG QI | MUHAMMAD IRFAN | JING-WEI LIN | HUI MA | ZHI-FU GUO | MING ZHONG | Tian-lai LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 3, pp.552-562

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811407

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152