Determination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs

Çağlar Kaya; Tolga Sarıyer; Esra Şahin

Makale detayları

Determination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs

Yazarlar:

Çağlar Kaya | Tolga Sarıyer | Esra Şahin

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Agricultural Engineering | Plant Biotechnology in Agriculture

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.345-348

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290040

pISSN:

2618-5946

Anahtar kelimeler:

Özet:

 Giant Snowdrop Galanthus elwesii Hook is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as  percent. For sterilization G. elwesii bulbs were sterilized at different concentrations 1, 3, 5, 7, 9, 11 and 13  of sodium hypochlorite NaOCl for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent  after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3 sodium hypochlorite solution however 100 contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11 sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.

 Giant Snowdrop Galanthus elwesii Hook is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as  percent. For sterilization G. elwesii bulbs were sterilized at different concentrations 1, 3, 5, 7, 9, 11 and 13  of sodium hypochlorite NaOCl for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent  after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3 sodium hypochlorite solution however 100 contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11 sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.

 Giant Snowdrop Galanthus elwesii Hook is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as  percent. For sterilization G. elwesii bulbs were sterilized at different concentrations 1, 3, 5, 7, 9, 11 and 13  of sodium hypochlorite NaOCl for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent  after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3 sodium hypochlorite solution however 100 contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11 sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.

 Giant Snowdrop Galanthus elwesii Hook is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as  percent. For sterilization G. elwesii bulbs were sterilized at different concentrations 1, 3, 5, 7, 9, 11 and 13  of sodium hypochlorite NaOCl for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent  after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3 sodium hypochlorite solution however 100 contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11 sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.

Makale detayları

Determination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs

Yazarlar:

Çağlar Kaya | Tolga Sarıyer | Esra Şahin

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Agricultural Engineering | Plant Biotechnology in Agriculture

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.345-348

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290040

pISSN:

2618-5946

Giant Snowdrop

Bulb

Sterilization

Contamination

DeformationGiant Snowdrop

Bulb

Sterilization

Contamination

Deformation

Sayı

Makale detayları

Yazarlar: Çağlar Kaya | Tolga Sarıyer | Esra Şahin

Yayıncı: Gültekin ÖZDEMİR

Ana Başlıklar: Agricultural Engineering | Plant Biotechnology in Agriculture

Dergi: International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.345-348

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001290040

pISSN: 2618-5946

Sayı

Yazarlar:

Çağlar Kaya | Tolga Sarıyer | Esra Şahin

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Agricultural Engineering | Plant Biotechnology in Agriculture

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.345-348

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290040

pISSN:

2618-5946