In Vitro Effects of Some Chemotherapeutic Drugs on Rat Erythrocytes Glutathione S-transferase (GST) Enzyme

Barzan Ahmed; Yusuf Temel; Mehmet Çiftci

Makale detayları

In Vitro Effects of Some Chemotherapeutic Drugs on Rat Erythrocytes Glutathione S-transferase (GST) Enzyme

Yazarlar:

Barzan Ahmed | Yusuf Temel | Mehmet Çiftci

Yayıncı:

Bingol University

Ana Başlıklar:

Enzimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 4, pp.17-22

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001336386

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 After heart disease, cancer is the leading cause of death worldwide. Currently, breast, lung, bowel, and prostate cancer are the most common cancers in the worldwide. By stopping cancer cells from dividing, spreading, growing, making more cells, and then destroying them, chemotherapy drugs are used to treat diseases caused by cancer. The glutathione S-transferase enzyme is responsible for the detoxification of xenobiotic molecules produced by the body during cancer treatment. In this study, glutathione S-transferase enzyme GST, EC: 2.5.1.18 was extracted from the erythrocytes of rats by affinity column chromatography in one step. The gel electrophoresis SDS-PAGE was used to verify the GST enzymes purity, A single protein band was obtained. The GST enzyme was purified with 22.5 EUmg specific activity, 237.14 purification-fold, and 48.98 purification yield. The subsequently investigation study into the in vitro effects of 5-fluorouracil 5-FU and cyclophosphamide CP chemotherapy drugs on purified GST enzyme activity revealed that both 5-fluorouracil and cyclophosphamide increased GST activity at a concentration range of 0.385 to 15.4 mM, and 19.15 to 191.5 mM respectively.

 After heart disease, cancer is the leading cause of death worldwide. Currently, breast, lung, bowel, and prostate cancer are the most common cancers in the worldwide. By stopping cancer cells from dividing, spreading, growing, making more cells, and then destroying them, chemotherapy drugs are used to treat diseases caused by cancer. The glutathione S-transferase enzyme is responsible for the detoxification of xenobiotic molecules produced by the body during cancer treatment. In this study, glutathione S-transferase enzyme GST, EC: 2.5.1.18 was extracted from the erythrocytes of rats by affinity column chromatography in one step. The gel electrophoresis SDS-PAGE was used to verify the GST enzymes purity, A single protein band was obtained. The GST enzyme was purified with 22.5 EUmg specific activity, 237.14 purification-fold, and 48.98 purification yield. The subsequently investigation study into the in vitro effects of 5-fluorouracil 5-FU and cyclophosphamide CP chemotherapy drugs on purified GST enzyme activity revealed that both 5-fluorouracil and cyclophosphamide increased GST activity at a concentration range of 0.385 to 15.4 mM, and 19.15 to 191.5 mM respectively.

 After heart disease, cancer is the leading cause of death worldwide. Currently, breast, lung, bowel, and prostate cancer are the most common cancers in the worldwide. By stopping cancer cells from dividing, spreading, growing, making more cells, and then destroying them, chemotherapy drugs are used to treat diseases caused by cancer. The glutathione S-transferase enzyme is responsible for the detoxification of xenobiotic molecules produced by the body during cancer treatment. In this study, glutathione S-transferase enzyme GST, EC: 2.5.1.18 was extracted from the erythrocytes of rats by affinity column chromatography in one step. The gel electrophoresis SDS-PAGE was used to verify the GST enzymes purity, A single protein band was obtained. The GST enzyme was purified with 22.5 EUmg specific activity, 237.14 purification-fold, and 48.98 purification yield. The subsequently investigation study into the in vitro effects of 5-fluorouracil 5-FU and cyclophosphamide CP chemotherapy drugs on purified GST enzyme activity revealed that both 5-fluorouracil and cyclophosphamide increased GST activity at a concentration range of 0.385 to 15.4 mM, and 19.15 to 191.5 mM respectively.

 After heart disease, cancer is the leading cause of death worldwide. Currently, breast, lung, bowel, and prostate cancer are the most common cancers in the worldwide. By stopping cancer cells from dividing, spreading, growing, making more cells, and then destroying them, chemotherapy drugs are used to treat diseases caused by cancer. The glutathione S-transferase enzyme is responsible for the detoxification of xenobiotic molecules produced by the body during cancer treatment. In this study, glutathione S-transferase enzyme GST, EC: 2.5.1.18 was extracted from the erythrocytes of rats by affinity column chromatography in one step. The gel electrophoresis SDS-PAGE was used to verify the GST enzymes purity, A single protein band was obtained. The GST enzyme was purified with 22.5 EUmg specific activity, 237.14 purification-fold, and 48.98 purification yield. The subsequently investigation study into the in vitro effects of 5-fluorouracil 5-FU and cyclophosphamide CP chemotherapy drugs on purified GST enzyme activity revealed that both 5-fluorouracil and cyclophosphamide increased GST activity at a concentration range of 0.385 to 15.4 mM, and 19.15 to 191.5 mM respectively.

Makale detayları

In Vitro Effects of Some Chemotherapeutic Drugs on Rat Erythrocytes Glutathione S-transferase (GST) Enzyme

Yazarlar:

Barzan Ahmed | Yusuf Temel | Mehmet Çiftci

Yayıncı:

Bingol University

Ana Başlıklar:

Enzimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 4, pp.17-22

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001336386

pISSN:

2149-6366

Rat erythrocyte

Glutathione S-transferase

Purification

Chemotherapeutic Drugs

Enzyme activity.

Sayı

Makale detayları

In Vitro Effects of Some Chemotherapeutic Drugs on Rat Erythrocytes Glutathione S-transferase (GST) Enzyme

Yazarlar: Barzan Ahmed | Yusuf Temel | Mehmet Çiftci

Yayıncı: Bingol University

Ana Başlıklar: Enzimler

Dergi: Türk Doğa ve Fen Dergisi Vol.13 Issue 4, pp.17-22

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001336386

pISSN: 2149-6366

Rat erythrocyte

Glutathione S-transferase

Purification

Chemotherapeutic Drugs

Enzyme activity.

Sayı

Yazarlar:

Barzan Ahmed | Yusuf Temel | Mehmet Çiftci

Yayıncı:

Bingol University

Ana Başlıklar:

Enzimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 4, pp.17-22

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001336386

pISSN:

2149-6366