Evaluation of a culture-independent gastrointestinal multiplex PCR panel for detection of gastrointestinal pathogens detection of gastrointestinal pathogens

Ömer Acer; Erdal Özbek; Selahattin Atmaca

Makale detayları

Evaluation of a culture-independent gastrointestinal multiplex PCR panel for detection of gastrointestinal pathogens detection of gastrointestinal pathogens

Yazarlar:

Ömer Acer | Erdal Özbek | Selahattin Atmaca

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Dicle Tıp Dergisi Vol.51 Issue 4, pp.470-476

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334147

pISSN:

1300-2945

Anahtar kelimeler:

Özet:

 Objective: All across the world, gastrointestinal GI infections are an important cause of morbidity and mortality, especially in young children, patients in intensive care units, and patients with weakened immune systems. In this study we aimed to assess the Gastroenteritis RT-qPCR MX-24T Panels utility as a standard technique for identifying gastrointestinal pathogens. Methods: In this study, 76 stool samples from intensive care patients were tested for bacterial, viral, and parasitic pathogens using the Bio-Speedy Gastroenteritis RT-qPCR MX-24T Panel kit. Results: In this study, 31 out of 76 samples gave positive results. Eight bacterial Salmonella spp., Campylobacter spp., ShigellaEnteroinvasive Escherichia coli EIEC, Enteroaggregative E. coli EAEC, Shiga toxin-producing E. coli STEC, Enteropathogenic E. coli EPEC, Enterotoxigenic E. coli ETEC, and Clostridium difficile binary toxin AB, three viral Astrovirus, Norovirus GIGII and Rotavirus A and two parasitic Cryptosporidium spp., and Giardia lamblia agents were detected from the stool samples of intensive care patients. While only a single agent was detected in the 22 samples, multiple agents were detected in 9 30. The most detected agent was EAEC n11, followed by Campylobacter spp. n7. EAEC and Campylobacter spp. were detected in 3 samples with multiple agents. Conclusion: The GI panel can minimize the need for additional diagnostic testing and unnecessary antibiotic use by rapidly identifying a wide range of infections detectable only by molecular methods, as well as agents detectable by traditional conventional diagnostic methods. In this way, it may lead to a shorter hospital stay. In addition, we think that further studies should be conducted to determine whether the simultaneous detection of multiple pathogens in a sample in our study is clinically important.

 Objective: All across the world, gastrointestinal GI infections are an important cause of morbidity and mortality, especially in young children, patients in intensive care units, and patients with weakened immune systems. In this study we aimed to assess the Gastroenteritis RT-qPCR MX-24T Panels utility as a standard technique for identifying gastrointestinal pathogens. Methods: In this study, 76 stool samples from intensive care patients were tested for bacterial, viral, and parasitic pathogens using the Bio-Speedy Gastroenteritis RT-qPCR MX-24T Panel kit. Results: In this study, 31 out of 76 samples gave positive results. Eight bacterial Salmonella spp., Campylobacter spp., ShigellaEnteroinvasive Escherichia coli EIEC, Enteroaggregative E. coli EAEC, Shiga toxin-producing E. coli STEC, Enteropathogenic E. coli EPEC, Enterotoxigenic E. coli ETEC, and Clostridium difficile binary toxin AB, three viral Astrovirus, Norovirus GIGII and Rotavirus A and two parasitic Cryptosporidium spp., and Giardia lamblia agents were detected from the stool samples of intensive care patients. While only a single agent was detected in the 22 samples, multiple agents were detected in 9 30. The most detected agent was EAEC n11, followed by Campylobacter spp. n7. EAEC and Campylobacter spp. were detected in 3 samples with multiple agents. Conclusion: The GI panel can minimize the need for additional diagnostic testing and unnecessary antibiotic use by rapidly identifying a wide range of infections detectable only by molecular methods, as well as agents detectable by traditional conventional diagnostic methods. In this way, it may lead to a shorter hospital stay. In addition, we think that further studies should be conducted to determine whether the simultaneous detection of multiple pathogens in a sample in our study is clinically important.

 Objective: All across the world, gastrointestinal GI infections are an important cause of morbidity and mortality, especially in young children, patients in intensive care units, and patients with weakened immune systems. In this study we aimed to assess the Gastroenteritis RT-qPCR MX-24T Panels utility as a standard technique for identifying gastrointestinal pathogens. Methods: In this study, 76 stool samples from intensive care patients were tested for bacterial, viral, and parasitic pathogens using the Bio-Speedy Gastroenteritis RT-qPCR MX-24T Panel kit. Results: In this study, 31 out of 76 samples gave positive results. Eight bacterial Salmonella spp., Campylobacter spp., ShigellaEnteroinvasive Escherichia coli EIEC, Enteroaggregative E. coli EAEC, Shiga toxin-producing E. coli STEC, Enteropathogenic E. coli EPEC, Enterotoxigenic E. coli ETEC, and Clostridium difficile binary toxin AB, three viral Astrovirus, Norovirus GIGII and Rotavirus A and two parasitic Cryptosporidium spp., and Giardia lamblia agents were detected from the stool samples of intensive care patients. While only a single agent was detected in the 22 samples, multiple agents were detected in 9 30. The most detected agent was EAEC n11, followed by Campylobacter spp. n7. EAEC and Campylobacter spp. were detected in 3 samples with multiple agents. Conclusion: The GI panel can minimize the need for additional diagnostic testing and unnecessary antibiotic use by rapidly identifying a wide range of infections detectable only by molecular methods, as well as agents detectable by traditional conventional diagnostic methods. In this way, it may lead to a shorter hospital stay. In addition, we think that further studies should be conducted to determine whether the simultaneous detection of multiple pathogens in a sample in our study is clinically important.

 Objective: All across the world, gastrointestinal GI infections are an important cause of morbidity and mortality, especially in young children, patients in intensive care units, and patients with weakened immune systems. In this study we aimed to assess the Gastroenteritis RT-qPCR MX-24T Panels utility as a standard technique for identifying gastrointestinal pathogens. Methods: In this study, 76 stool samples from intensive care patients were tested for bacterial, viral, and parasitic pathogens using the Bio-Speedy Gastroenteritis RT-qPCR MX-24T Panel kit. Results: In this study, 31 out of 76 samples gave positive results. Eight bacterial Salmonella spp., Campylobacter spp., ShigellaEnteroinvasive Escherichia coli EIEC, Enteroaggregative E. coli EAEC, Shiga toxin-producing E. coli STEC, Enteropathogenic E. coli EPEC, Enterotoxigenic E. coli ETEC, and Clostridium difficile binary toxin AB, three viral Astrovirus, Norovirus GIGII and Rotavirus A and two parasitic Cryptosporidium spp., and Giardia lamblia agents were detected from the stool samples of intensive care patients. While only a single agent was detected in the 22 samples, multiple agents were detected in 9 30. The most detected agent was EAEC n11, followed by Campylobacter spp. n7. EAEC and Campylobacter spp. were detected in 3 samples with multiple agents. Conclusion: The GI panel can minimize the need for additional diagnostic testing and unnecessary antibiotic use by rapidly identifying a wide range of infections detectable only by molecular methods, as well as agents detectable by traditional conventional diagnostic methods. In this way, it may lead to a shorter hospital stay. In addition, we think that further studies should be conducted to determine whether the simultaneous detection of multiple pathogens in a sample in our study is clinically important.

Makale detayları

Evaluation of a culture-independent gastrointestinal multiplex PCR panel for detection of gastrointestinal pathogens detection of gastrointestinal pathogens

Yazarlar:

Ömer Acer | Erdal Özbek | Selahattin Atmaca

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Dicle Tıp Dergisi Vol.51 Issue 4, pp.470-476

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334147

pISSN:

1300-2945

Gastrointestinal

infection

molecular panel

multiplex polymerase chain reaction

Sayı

Makale detayları

Evaluation of a culture-independent gastrointestinal multiplex PCR panel for detection of gastrointestinal pathogens detection of gastrointestinal pathogens

Yazarlar: Ömer Acer | Erdal Özbek | Selahattin Atmaca

Yayıncı: Dicle University

Ana Başlıklar: Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: Dicle Tıp Dergisi Vol.51 Issue 4, pp.470-476

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001334147

pISSN: 1300-2945

Gastrointestinal

infection

molecular panel

multiplex polymerase chain reaction

Sayı

Yazarlar:

Ömer Acer | Erdal Özbek | Selahattin Atmaca

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi | Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Dicle Tıp Dergisi Vol.51 Issue 4, pp.470-476

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334147

pISSN:

1300-2945