E-microsatellite markers for some naturally occurring Salvia species in the Mediterranean region

Ayşe Gül İNCE; Mehmet KARACA

Makale detayları

E-microsatellite markers for some naturally occurring Salvia species in the Mediterranean region

Yazarlar:

Ayşe Gül İNCE | Mehmet KARACA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.69-77

Belge Tipi:

Makale

Yayın Tarihi:

3/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000812020

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The genus Salvia L. contains about 1000 species, more than 40 of which are endemic to the Mediterranean region. Species boundaries and classifications of this genus have been mainly conducted based on morphological and anatomical characteristics. Although genomic and genic microsatellites are commonly used in a variety of genetic studies in many plant and animal species, their use has been limited in Salvia species. This research was undertaken to study microsatellite contents of expressed sequence tags (ESTs) and develop a new set of expressed-microsatellite (e-microsatellite) markers using the GenBank databases. In in silico studies of the research, microsatellites were identified and e-microsatellite primer pairs flanking the repeats were designed. In in vitro studies of the present work, e-microsatellite primer pairs were used to amplify genomic DNA samples of different Salvia species naturally occurring in the Mediterranean region of Turkey. The results indicated that ESTs of related species deposited in GenBank were reliable sources for the development of e-microsatellite markers. The results of this study also indicated that cross-transferability of e-microsatellite markers decreased as the evolutionary distance among the tested Salvia species increased. E-microsatellite loci presented in this work could be very useful for measuring genetic diversity, gene flow, and species identification studies in the genus Salvia.

 

The genus Salvia L. contains about 1000 species, more than 40 of which are endemic to the Mediterranean region. Species boundaries and classifications of this genus have been mainly conducted based on morphological and anatomical characteristics. Although genomic and genic microsatellites are commonly used in a variety of genetic studies in many plant and animal species, their use has been limited in Salvia species. This research was undertaken to study microsatellite contents of expressed sequence tags (ESTs) and develop a new set of expressed-microsatellite (e-microsatellite) markers using the GenBank databases. In in silico studies of the research, microsatellites were identified and e-microsatellite primer pairs flanking the repeats were designed. In in vitro studies of the present work, e-microsatellite primer pairs were used to amplify genomic DNA samples of different Salvia species naturally occurring in the Mediterranean region of Turkey. The results indicated that ESTs of related species deposited in GenBank were reliable sources for the development of e-microsatellite markers. The results of this study also indicated that cross-transferability of e-microsatellite markers decreased as the evolutionary distance among the tested Salvia species increased. E-microsatellite loci presented in this work could be very useful for measuring genetic diversity, gene flow, and species identification studies in the genus Salvia.

Makale detayları

E-microsatellite markers for some naturally occurring Salvia species in the Mediterranean region

Yazarlar:

Ayşe Gül İNCE | Mehmet KARACA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.69-77

Belge Tipi:

Makale

Yayın Tarihi:

3/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000812020

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Cross-transferability

DNA markers

expressed sequence tags

Labiatae

sage

touch-down-PCR

Sayı

Makale detayları

E-microsatellite markers for some naturally occurring Salvia species in the Mediterranean region

Yazarlar: Ayşe Gül İNCE | Mehmet KARACA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.69-77

Belge Tipi: Makale

Yayın Tarihi: 3/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000812020

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Cross-transferability

DNA markers

expressed sequence tags

Labiatae

sage

touch-down-PCR

Sayı

Yazarlar:

Ayşe Gül İNCE | Mehmet KARACA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.69-77

Belge Tipi:

Makale

Yayın Tarihi:

3/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000812020

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152