Development of Pink Tomato Genitors Resistant or Tolerant to Tomato Root-Knot Nematode (Meloidogyne spp.) and Tomato Spotted Wilt Virus (TSWV) Using Molecular Markers

Hüseyin Basım; Osman Kandil; Cansu Ökem

Makale detayları

Development of Pink Tomato Genitors Resistant or Tolerant to Tomato Root-Knot Nematode (Meloidogyne spp.) and Tomato Spotted Wilt Virus (TSWV) Using Molecular Markers

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.39-49

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359193

pISSN:

2791-8807

Anahtar kelimeler:

Özet:

 Tomato Solanum lycopersicum production faces increasing threats from biotic stress factors such as Root-knot Nematodes Meloidogyne spp. and Tomato Spotted Wilt Virus TSWV, which can cause significant yield losses. In this study, molecular characterization of 154 tomato genotypes was performed to assess their resistance and tolerance to Root-knot Nematodes Meloidogyne spp. and Tomato Spotted Wilt Virus TSWV using Polymerase Chain Reaction PCR with SCAR markers of Mi23 and Sw5 specific to the target pathogens. PCR results successfully categorized the genotypes into resistant, heterozygous resistanttolerant, and susceptible groups. The Mi23 marker detected genotypes carrying the mi-1.2 gene associated with resistance to Root-knot Nematodes, while the Sw5 marker identified those resistant to TSWV. The results showed that several tomato lines have both resistance and tolerance mechanisms, highlighting their potential value in breeding programs aimed at developing new cultivars with better resistance to multiple biotic stresses. While molecular data offered useful insights, field-based pathogenicity tests are needed to confirm the resistance traits under different environmental conditions. Overall, this study highlights the effectiveness of marker-assisted selection MAS in speeding up the identification of resistant lines and significantly advances sustainable tomato breeding strategies to minimize yield losses caused by pathogens.

 Tomato Solanum lycopersicum production faces increasing threats from biotic stress factors such as Root-knot Nematodes Meloidogyne spp. and Tomato Spotted Wilt Virus TSWV, which can cause significant yield losses. In this study, molecular characterization of 154 tomato genotypes was performed to assess their resistance and tolerance to Root-knot Nematodes Meloidogyne spp. and Tomato Spotted Wilt Virus TSWV using Polymerase Chain Reaction PCR with SCAR markers of Mi23 and Sw5 specific to the target pathogens. PCR results successfully categorized the genotypes into resistant, heterozygous resistanttolerant, and susceptible groups. The Mi23 marker detected genotypes carrying the mi-1.2 gene associated with resistance to Root-knot Nematodes, while the Sw5 marker identified those resistant to TSWV. The results showed that several tomato lines have both resistance and tolerance mechanisms, highlighting their potential value in breeding programs aimed at developing new cultivars with better resistance to multiple biotic stresses. While molecular data offered useful insights, field-based pathogenicity tests are needed to confirm the resistance traits under different environmental conditions. Overall, this study highlights the effectiveness of marker-assisted selection MAS in speeding up the identification of resistant lines and significantly advances sustainable tomato breeding strategies to minimize yield losses caused by pathogens.

 Tomato Solanum lycopersicum production faces increasing threats from biotic stress factors such as Root-knot Nematodes Meloidogyne spp. and Tomato Spotted Wilt Virus TSWV, which can cause significant yield losses. In this study, molecular characterization of 154 tomato genotypes was performed to assess their resistance and tolerance to Root-knot Nematodes Meloidogyne spp. and Tomato Spotted Wilt Virus TSWV using Polymerase Chain Reaction PCR with SCAR markers of Mi23 and Sw5 specific to the target pathogens. PCR results successfully categorized the genotypes into resistant, heterozygous resistanttolerant, and susceptible groups. The Mi23 marker detected genotypes carrying the mi-1.2 gene associated with resistance to Root-knot Nematodes, while the Sw5 marker identified those resistant to TSWV. The results showed that several tomato lines have both resistance and tolerance mechanisms, highlighting their potential value in breeding programs aimed at developing new cultivars with better resistance to multiple biotic stresses. While molecular data offered useful insights, field-based pathogenicity tests are needed to confirm the resistance traits under different environmental conditions. Overall, this study highlights the effectiveness of marker-assisted selection MAS in speeding up the identification of resistant lines and significantly advances sustainable tomato breeding strategies to minimize yield losses caused by pathogens.

 Domates Solanum lycopersicum üretimi, önemli verim kayıplarına neden olabilen Kök-ur Nematodları Meloidogyne spp. ve Domates Benekli Solgunluk Virüsü TSWV gibi biyotik stres faktörlerinden kaynaklanan artan tehditlerle karşı karşıyadır. Bu çalışmada, Kök-ur Nematodları Meloidogyne spp. ve Domates Benekli Solgunluk Virüsüne TSWV karşı direnç ve toleranslarını değerlendirmek için 154 domates genotipinin moleküler karakterizasyonu, hedef patojenlere özgü Mi23 ve Sw5 SCAR belirteçleri ile Polimeraz Zincir Reaksiyonu PCR kullanılarak gerçekleştirilmiştir. PCR sonuçları genotipleri dirençli, heterozigot dirençlitoleranslı ve duyarlı gruplara başarıyla kategorize etmiştir. Mi23 belirteci, Kök-ur Nematodlarına dirençle ilişkili mi-1.2 genini taşıyan genotipleri tespit ederken, Sw5 belirteci TSWVye dirençli olanları belirlemiştir. Sonuçlar, birçok domates hattının hem dayanıklı hem de tolerantlı olduğunu göstererek, çoklu biyotik stres faktörüne karşı daha iyi dayanıklı yeni çeşitlerin geliştirilmesini amaçlayan ıslah programlarındaki potansiyel değerlerini vurgulamaktadır. Moleküler veriler faydalı bilgiler sunarken, farklı çevre koşullarında dayanıklılık özelliklerini doğrulamak için arazi bazlı patojenite testlerine ihtiyaç duyulmaktadır. Genel olarak, bu çalışma, dayanıklı domates hatların belirlenmesini hızlandırmada markör destekli seleksiyonun MAS etkinliğini vurgulamakta ve patojenlerin neden olduğu verim kayıplarını en aza indirmede sürdürülebilir domates ıslah stratejilerini önemli ölçüde geliştirebilir.

Makale detayları

Development of Pink Tomato Genitors Resistant or Tolerant to Tomato Root-Knot Nematode (Meloidogyne spp.) and Tomato Spotted Wilt Virus (TSWV) Using Molecular Markers

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.39-49

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359193

pISSN:

2791-8807

Mi23

Nematode

Sw5

Tomato

TSWVDomates

MAS

Mi23

Nematod

Sw5

TSWV

Sayı

Makale detayları

Yazarlar: Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı: Bingol University

Ana Başlıklar: Fitopatoloji

Dergi: Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.39-49

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359193

pISSN: 2791-8807

Sayı

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.39-49

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359193

pISSN:

2791-8807