EXPLORING THE ROLE OF sFRP-4, TFF-3, NF-кB AND ROMO1 LEVELS IN COLORECTAL CANCER: IMPLICATIONS FOR PATHOPHYSIOLOGY AND DISEASE PROGRESSION

Sinem Durmuş; Berrin Papila Kundaktepe; Remise Gelişgen; Çiğdem Papila; Hafize Uzun

Makale detayları

EXPLORING THE ROLE OF sFRP-4, TFF-3, NF-кB AND ROMO1 LEVELS IN COLORECTAL CANCER: IMPLICATIONS FOR PATHOPHYSIOLOGY AND DISEASE PROGRESSION

Yazarlar:

Sinem Durmuş | Berrin Papila Kundaktepe | Remise Gelişgen | Çiğdem Papila | Hafize Uzun

Yayıncı:

Kocaeli University

Ana Başlıklar:

Proteomik ve Moleküller Arası Etkileşimler

Dergi:

Acta Medica Nicomedia Vol.7 Issue 1, pp.120-126

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312617

pISSN:

2717-8994

Anahtar kelimeler:

Özet:

 Objective: Colorectal cancer CRC is a significant global health concern with high morbidity and mortality rates. Early detection and accurate diagnostic tools are critical for managing the clinical course. This research explores the molecular landscape of CRC, aiming to provide valuable insights beyond traditional diagnostic approaches. The main aim of this study was to investigate the potential contribution of specific biomarkers, such as secreted frizzled associated protein-4 sFRP-4, trefoil factor-3 TFF-3, nuclear factor-kappa-B NF-κB and reactive oxygen species modulator-1 Romo1, to understanding the pathophysiology and determining the progression of CRC. Methods: This study analyzed plasma levels of sFRP-4, TFF-3, NF-κB and Romo1 in a cohort of patients with CRC n50 and age- and gender-matched control group n40, utilizing ELISA. The diagnostic performance of these biomarkers was assessed through Receiver Operating Characteristic ROC analysis. Results: Our research revealed a significant increase in the levels of NF-κB, TFF-3 and Romo1 in patients with a diagnosis of CRC. Furthermore, these parameters were found to maintain elevated levels in patients with tumors larger than 4 cm as opposed to those with smaller tumors. Patients with metastases also had elevated levels of the three parameters compared with patients without metastases. The ROC analysis revealed that NF-κB showed the most promise as a parameter for distinguishing patients from control subjects, whereas TFF-3 displayed the most potential in identifying tumor size and the presence of metastasis. Conclusion: This research contributes valuable insights into understanding the pathophysiology and progression of CRC. The potential roles of NF-κB, TFF-3, and Romo1 as biomarkers, as revealed in our study, offer a promising avenue for early detection and improved management of CRC. Further validation and prospective studies are necessary to clarify the roles of these biomarkers in the pathophysiological mechanism of CRC and to establish their clinical utility.

 Objective: Colorectal cancer CRC is a significant global health concern with high morbidity and mortality rates. Early detection and accurate diagnostic tools are critical for managing the clinical course. This research explores the molecular landscape of CRC, aiming to provide valuable insights beyond traditional diagnostic approaches. The main aim of this study was to investigate the potential contribution of specific biomarkers, such as secreted frizzled associated protein-4 sFRP-4, trefoil factor-3 TFF-3, nuclear factor-kappa-B NF-κB and reactive oxygen species modulator-1 Romo1, to understanding the pathophysiology and determining the progression of CRC. Methods: This study analyzed plasma levels of sFRP-4, TFF-3, NF-κB and Romo1 in a cohort of patients with CRC n50 and age- and gender-matched control group n40, utilizing ELISA. The diagnostic performance of these biomarkers was assessed through Receiver Operating Characteristic ROC analysis. Results: Our research revealed a significant increase in the levels of NF-κB, TFF-3 and Romo1 in patients with a diagnosis of CRC. Furthermore, these parameters were found to maintain elevated levels in patients with tumors larger than 4 cm as opposed to those with smaller tumors. Patients with metastases also had elevated levels of the three parameters compared with patients without metastases. The ROC analysis revealed that NF-κB showed the most promise as a parameter for distinguishing patients from control subjects, whereas TFF-3 displayed the most potential in identifying tumor size and the presence of metastasis. Conclusion: This research contributes valuable insights into understanding the pathophysiology and progression of CRC. The potential roles of NF-κB, TFF-3, and Romo1 as biomarkers, as revealed in our study, offer a promising avenue for early detection and improved management of CRC. Further validation and prospective studies are necessary to clarify the roles of these biomarkers in the pathophysiological mechanism of CRC and to establish their clinical utility.

 Objective: Colorectal cancer CRC is a significant global health concern with high morbidity and mortality rates. Early detection and accurate diagnostic tools are critical for managing the clinical course. This research explores the molecular landscape of CRC, aiming to provide valuable insights beyond traditional diagnostic approaches. The main aim of this study was to investigate the potential contribution of specific biomarkers, such as secreted frizzled associated protein-4 sFRP-4, trefoil factor-3 TFF-3, nuclear factor-kappa-B NF-κB and reactive oxygen species modulator-1 Romo1, to understanding the pathophysiology and determining the progression of CRC. Methods: This study analyzed plasma levels of sFRP-4, TFF-3, NF-κB and Romo1 in a cohort of patients with CRC n50 and age- and gender-matched control group n40, utilizing ELISA. The diagnostic performance of these biomarkers was assessed through Receiver Operating Characteristic ROC analysis. Results: Our research revealed a significant increase in the levels of NF-κB, TFF-3 and Romo1 in patients with a diagnosis of CRC. Furthermore, these parameters were found to maintain elevated levels in patients with tumors larger than 4 cm as opposed to those with smaller tumors. Patients with metastases also had elevated levels of the three parameters compared with patients without metastases. The ROC analysis revealed that NF-κB showed the most promise as a parameter for distinguishing patients from control subjects, whereas TFF-3 displayed the most potential in identifying tumor size and the presence of metastasis. Conclusion: This research contributes valuable insights into understanding the pathophysiology and progression of CRC. The potential roles of NF-κB, TFF-3, and Romo1 as biomarkers, as revealed in our study, offer a promising avenue for early detection and improved management of CRC. Further validation and prospective studies are necessary to clarify the roles of these biomarkers in the pathophysiological mechanism of CRC and to establish their clinical utility.

 Amaç: Kolorektal kanser KRK, yüksek morbidite ve mortalite oranları ile önemli bir küresel sağlık sorunudur. Erken teşhis ve doğru tanı araçları klinik seyrin yönetimi için kritik öneme sahiptir. Bu araştırma, geleneksel tanı yaklaşımlarının ötesinde bilgiler sağlamayı amaçlayarak KRKnin moleküler manzarasını araştırmaktadır. Bu çalışmanın temel amacı, salgılanan frizzled ilişkili protein-4 sFRP-4, trefoil faktör-3 TFF-3, nükleer faktör-kappa-B NF-κB ve reaktif oksijen türleri modülatörü-1 Romo1 gibi spesifik biyobelirteçlerin, KRKnin patofizyolojisini anlamaya ve progresyonunu belirlemeye potansiyel katkılarını araştırmaktır. Yöntem: Bu çalışmada, KRKli hastalar n50 ile yaş ve cinsiyet açısından eşleştirilmiş kontrol grubunda n40 sFRP-4, TFF-3, NF-κB ve Romo1in plazma düzeyleri ELISA yöntemi ile analiz edilmiştir. Bu biyobelirteçlerin tanısal performansı ROC analizi ile değerlendirilmiştir. Bulgular: Araştırmamız, KRK tanısı alan hastalarda NF-κB, TFF-3 ve Romo1 seviyelerinde önemli bir artış olduğunu ortaya koymuştur. Ayrıca, bu parametrelerin 4 cmden büyük tümörü olan hastalarda daha küçük tümörü olanlara kıyasla daha yüksek seviyelerde olduğu tespit edilmiştir. Metastazı olan hastalarda da metastazı olmayan hastalara kıyasla üç parametrenin seviyeleri de yüksek bulunmuştur. ROC analizi, NF-κBnin hastaları kontrol bireylerinden ayırt etmek için potansiyeli en iyi biyobelirteç olabileceğini, TFF-3ün ise tümör boyutunu ve metastaz varlığını belirlemede en fazla potansiyel gösterdiğini ortaya koymuştur. Sonuç: Bu araştırma, KRKnın patofizyolojisinin ve progresyonunun anlaşılmasına katkı sağlamaktadır. Çalışmamızda ortaya konduğu üzere NF-κB, TFF-3 ve Romo1in biyobelirteç olarak potansiyel rolleri, KRKnin erken teşhisi ve yönetimi için umut verici bir yaklaşım sunmaktadır. Bu biyobelirteçlerin KRKnin patofizyolojik mekanizmasındaki rollerini netleştirmek ve klinik faydalarını belirlemek için daha fazla doğrulama ve ileriye dönük çalışmalar gereklidir.

Makale detayları

EXPLORING THE ROLE OF sFRP-4, TFF-3, NF-кB AND ROMO1 LEVELS IN COLORECTAL CANCER: IMPLICATIONS FOR PATHOPHYSIOLOGY AND DISEASE PROGRESSION

Yazarlar:

Sinem Durmuş | Berrin Papila Kundaktepe | Remise Gelişgen | Çiğdem Papila | Hafize Uzun

Yayıncı:

Kocaeli University

Ana Başlıklar:

Proteomik ve Moleküller Arası Etkileşimler

Dergi:

Acta Medica Nicomedia Vol.7 Issue 1, pp.120-126

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312617

pISSN:

2717-8994

Colorectal cancer

NF-κB

Romo1

sFRP-4

TFF-3Kolorektal kanser

NF-κB

Romo1

sFRP-4

TFF-3

Sayı

Makale detayları

Yazarlar: Sinem Durmuş | Berrin Papila Kundaktepe | Remise Gelişgen | Çiğdem Papila | Hafize Uzun

Yayıncı: Kocaeli University

Ana Başlıklar: Proteomik ve Moleküller Arası Etkileşimler

Dergi: Acta Medica Nicomedia Vol.7 Issue 1, pp.120-126

Belge Tipi: Makale

Yayın Tarihi: 2/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001312617

pISSN: 2717-8994

Sayı

Yazarlar:

Sinem Durmuş | Berrin Papila Kundaktepe | Remise Gelişgen | Çiğdem Papila | Hafize Uzun

Yayıncı:

Kocaeli University

Ana Başlıklar:

Proteomik ve Moleküller Arası Etkileşimler

Dergi:

Acta Medica Nicomedia Vol.7 Issue 1, pp.120-126

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312617

pISSN:

2717-8994