Social isolation and predator scent tests alter brain BDNF levels differentially according to gender, in rats and effects of fluoxetine

Aslı Aykaç; Şule Öncül

Makale detayları

Social isolation and predator scent tests alter brain BDNF levels differentially according to gender, in rats and effects of fluoxetine

Yazarlar:

Aslı Aykaç | Şule Öncül

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.190-198

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346172

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 We compared different stress conditions on brain BDNF levels, in rats exposed to social isolation test SIT and predator scent tests PST. BDNF expression in the frontal cortex, hippocampus, and amygdala was compared, and effects of chronic fluoxetine FLU treatment were evaluated. Rats were exposed to SIT and PST for one month. FLU was given 5 mgkgday, ip throughout stress procedures. Controls, stress, and treatment groups were evaluated in elevated plus maze, anxiety scores were calculated. BDNF expression was determined by Western blot. SIT and PST induced anxiety in both sexes, females had greater anxiety scores than males p0.05. FLU restored anxiety scores in both sexes p0.01 in both tests. Male and female rats exhibited reduced cortical BDNF levels in SIT p0.001. PST reduced cortical BDNF in females, but increased in males. Hippocampal BDNF expression was lowered in SIT p0.01 and PST p0.001 in both sexes. Female rats had 40 lower BDNF expression than males in the amygdala in SIT. FLU did not restore cortical BDNF in females in both tests, but reduced increased BDNF levels in males in PST p0.001. FLU did not restore reduced brain BDNF in males in the hippocampus and amygdala, but restored in hippocampus, in females. Our findings indicate that sex differences must be considered in studies related to mood disorders of animal models, and suggest that BDNF expression in different brain regions are altered differentially in a gender-dependent manner in rats. Antianxiety effect of FLU is not mediated through increasing BDNF activity in cortex in both genders. Increased BDNF in hippocampus and amygdala may reflect antidepressant effect of FLU in female rats, but not in males.

 We compared different stress conditions on brain BDNF levels, in rats exposed to social isolation test SIT and predator scent tests PST. BDNF expression in the frontal cortex, hippocampus, and amygdala was compared, and effects of chronic fluoxetine FLU treatment were evaluated. Rats were exposed to SIT and PST for one month. FLU was given 5 mgkgday, ip throughout stress procedures. Controls, stress, and treatment groups were evaluated in elevated plus maze, anxiety scores were calculated. BDNF expression was determined by Western blot. SIT and PST induced anxiety in both sexes, females had greater anxiety scores than males p0.05. FLU restored anxiety scores in both sexes p0.01 in both tests. Male and female rats exhibited reduced cortical BDNF levels in SIT p0.001. PST reduced cortical BDNF in females, but increased in males. Hippocampal BDNF expression was lowered in SIT p0.01 and PST p0.001 in both sexes. Female rats had 40 lower BDNF expression than males in the amygdala in SIT. FLU did not restore cortical BDNF in females in both tests, but reduced increased BDNF levels in males in PST p0.001. FLU did not restore reduced brain BDNF in males in the hippocampus and amygdala, but restored in hippocampus, in females. Our findings indicate that sex differences must be considered in studies related to mood disorders of animal models, and suggest that BDNF expression in different brain regions are altered differentially in a gender-dependent manner in rats. Antianxiety effect of FLU is not mediated through increasing BDNF activity in cortex in both genders. Increased BDNF in hippocampus and amygdala may reflect antidepressant effect of FLU in female rats, but not in males.

 We compared different stress conditions on brain BDNF levels, in rats exposed to social isolation test SIT and predator scent tests PST. BDNF expression in the frontal cortex, hippocampus, and amygdala was compared, and effects of chronic fluoxetine FLU treatment were evaluated. Rats were exposed to SIT and PST for one month. FLU was given 5 mgkgday, ip throughout stress procedures. Controls, stress, and treatment groups were evaluated in elevated plus maze, anxiety scores were calculated. BDNF expression was determined by Western blot. SIT and PST induced anxiety in both sexes, females had greater anxiety scores than males p0.05. FLU restored anxiety scores in both sexes p0.01 in both tests. Male and female rats exhibited reduced cortical BDNF levels in SIT p0.001. PST reduced cortical BDNF in females, but increased in males. Hippocampal BDNF expression was lowered in SIT p0.01 and PST p0.001 in both sexes. Female rats had 40 lower BDNF expression than males in the amygdala in SIT. FLU did not restore cortical BDNF in females in both tests, but reduced increased BDNF levels in males in PST p0.001. FLU did not restore reduced brain BDNF in males in the hippocampus and amygdala, but restored in hippocampus, in females. Our findings indicate that sex differences must be considered in studies related to mood disorders of animal models, and suggest that BDNF expression in different brain regions are altered differentially in a gender-dependent manner in rats. Antianxiety effect of FLU is not mediated through increasing BDNF activity in cortex in both genders. Increased BDNF in hippocampus and amygdala may reflect antidepressant effect of FLU in female rats, but not in males.

 We compared different stress conditions on brain BDNF levels, in rats exposed to social isolation test SIT and predator scent tests PST. BDNF expression in the frontal cortex, hippocampus, and amygdala was compared, and effects of chronic fluoxetine FLU treatment were evaluated. Rats were exposed to SIT and PST for one month. FLU was given 5 mgkgday, ip throughout stress procedures. Controls, stress, and treatment groups were evaluated in elevated plus maze, anxiety scores were calculated. BDNF expression was determined by Western blot. SIT and PST induced anxiety in both sexes, females had greater anxiety scores than males p0.05. FLU restored anxiety scores in both sexes p0.01 in both tests. Male and female rats exhibited reduced cortical BDNF levels in SIT p0.001. PST reduced cortical BDNF in females, but increased in males. Hippocampal BDNF expression was lowered in SIT p0.01 and PST p0.001 in both sexes. Female rats had 40 lower BDNF expression than males in the amygdala in SIT. FLU did not restore cortical BDNF in females in both tests, but reduced increased BDNF levels in males in PST p0.001. FLU did not restore reduced brain BDNF in males in the hippocampus and amygdala, but restored in hippocampus, in females. Our findings indicate that sex differences must be considered in studies related to mood disorders of animal models, and suggest that BDNF expression in different brain regions are altered differentially in a gender-dependent manner in rats. Antianxiety effect of FLU is not mediated through increasing BDNF activity in cortex in both genders. Increased BDNF in hippocampus and amygdala may reflect antidepressant effect of FLU in female rats, but not in males.

Makale detayları

Social isolation and predator scent tests alter brain BDNF levels differentially according to gender, in rats and effects of fluoxetine

Yazarlar: Aslı Aykaç | Şule Öncül

Yayıncı: Marmara University

Ana Başlıklar: Basic Pharmacology

Dergi: Journal of Research in Pharmacy Vol.22 Issue 2, pp.190-198

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346172

pISSN: 2630-6344

fluoxetine

BDNF

predator scent test

social isolation test

hippocampus

Sayı

Yazarlar:

Aslı Aykaç | Şule Öncül

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.190-198

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346172

pISSN:

2630-6344