Ovarian Ischemia-Reperfusion Injury and the Potential Role of Mangiferin: A Histopathological, Biochemical Investigation

Hayat Ayaz; Fırat Aşır

Makale detayları

Ovarian Ischemia-Reperfusion Injury and the Potential Role of Mangiferin: A Histopathological, Biochemical Investigation

Yazarlar:

Hayat Ayaz | Fırat Aşır

Yayıncı:

Kocaeli University

Ana Başlıklar:

Kadın Hastalıkları ve Doğum

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.11 Issue 3, pp.160-166

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359762

pISSN:

2149-8571

Anahtar kelimeler:

Özet:

 Objective: Ischemia-reperfusion IR injury in the ovary can cause significant structural damage and compromise ovarian reserve by disrupting follicular integrity. This study aimed to investigate the protective effects of Mangiferin, a natural antioxidant and anti-inflammatory compound, in a rat model of ovarian IR injury. Histopathological evaluation, follicle counts, and serum anti-Müllerian hormone AMH levels were used to assess treatment efficacy. Methods: Thirty-two female Sprague-Dawley rats were divided into Control, Mangiferin, IR, and IR Mangiferin. Ovarian IR was induced by bilateral clamping of the ovarian vasculature for 3 hours, followed by 3 hours of reperfusion. Mangiferin 20 mgkg was administered intraperitoneally once daily for 15 days. Ovarian tissues were subjected to histological analysis with follicle counts and AMH levels were measured using ELISA. Results: The IR group exhibited marked histopathological changes, including vascular congestion, hemorrhage, edema, inflammatory cell infiltration, and extensive follicular degeneration. In contrast, the IR Mangiferin group showed improved tissue architecture, higher follicle counts, and AMH levels that were significantly preserved compared to the IR group. Conclusion: Mangiferin may reduce ovarian damage caused by IR injury and contribute to the preservation of ovarian reserve. These findings suggest a potential therapeutic benefit of Mangiferin in protecting ovarian tissue under ischemic conditions.

 Objective: Ischemia-reperfusion IR injury in the ovary can cause significant structural damage and compromise ovarian reserve by disrupting follicular integrity. This study aimed to investigate the protective effects of Mangiferin, a natural antioxidant and anti-inflammatory compound, in a rat model of ovarian IR injury. Histopathological evaluation, follicle counts, and serum anti-Müllerian hormone AMH levels were used to assess treatment efficacy. Methods: Thirty-two female Sprague-Dawley rats were divided into Control, Mangiferin, IR, and IR Mangiferin. Ovarian IR was induced by bilateral clamping of the ovarian vasculature for 3 hours, followed by 3 hours of reperfusion. Mangiferin 20 mgkg was administered intraperitoneally once daily for 15 days. Ovarian tissues were subjected to histological analysis with follicle counts and AMH levels were measured using ELISA. Results: The IR group exhibited marked histopathological changes, including vascular congestion, hemorrhage, edema, inflammatory cell infiltration, and extensive follicular degeneration. In contrast, the IR Mangiferin group showed improved tissue architecture, higher follicle counts, and AMH levels that were significantly preserved compared to the IR group. Conclusion: Mangiferin may reduce ovarian damage caused by IR injury and contribute to the preservation of ovarian reserve. These findings suggest a potential therapeutic benefit of Mangiferin in protecting ovarian tissue under ischemic conditions.

 Objective: Ischemia-reperfusion IR injury in the ovary can cause significant structural damage and compromise ovarian reserve by disrupting follicular integrity. This study aimed to investigate the protective effects of Mangiferin, a natural antioxidant and anti-inflammatory compound, in a rat model of ovarian IR injury. Histopathological evaluation, follicle counts, and serum anti-Müllerian hormone AMH levels were used to assess treatment efficacy. Methods: Thirty-two female Sprague-Dawley rats were divided into Control, Mangiferin, IR, and IR Mangiferin. Ovarian IR was induced by bilateral clamping of the ovarian vasculature for 3 hours, followed by 3 hours of reperfusion. Mangiferin 20 mgkg was administered intraperitoneally once daily for 15 days. Ovarian tissues were subjected to histological analysis with follicle counts and AMH levels were measured using ELISA. Results: The IR group exhibited marked histopathological changes, including vascular congestion, hemorrhage, edema, inflammatory cell infiltration, and extensive follicular degeneration. In contrast, the IR Mangiferin group showed improved tissue architecture, higher follicle counts, and AMH levels that were significantly preserved compared to the IR group. Conclusion: Mangiferin may reduce ovarian damage caused by IR injury and contribute to the preservation of ovarian reserve. These findings suggest a potential therapeutic benefit of Mangiferin in protecting ovarian tissue under ischemic conditions.

 Amaç: Overde gelişen iskemi-reperfüzyon IR hasarı, folikül bütünlüğünü bozarak belirgin doku hasarına ve over rezervinin azalmasına yol açabilir. Bu çalışmada, Mangiferinin IR kaynaklı over hasarına karşı olası koruyucu etkilerinin; histopatolojik değişiklikler, folikül sayısı ve serum Anti-Müllerian Hormon AMH düzeyleri üzerinden değerlendirilmesi amaçlanmıştır. Yöntem: Otuz iki dişi Sprague-Dawley sıçan dört gruba ayrılmıştır: Kontrol, Mangiferin, IR ve IRMangiferin. Over IR modeli, bilateral over vaskülatürünün üç saat süreyle kleplenmesi ve ardından üç saatlik reperfüzyon uygulanması ile oluşturulmuştur. Mangiferin 20 mgkg, 15 gün boyunca intraperitoneal yolla günde bir kez uygulanmıştır. Over dokuları histopatolojik incelemeye tabi tutulmuş; folikül sayımları gerçekleştirilmiş ve serum AMH düzeyleri ELISA yöntemiyle analiz edilmiştir. Bulgular: IR grubunda vasküler konjesyon, hemoraji, ödem, inflamatuar hücre infiltrasyonu ve belirgin folikül dejenerasyonu gibi ciddi histopatolojik bozulmalar gözlemlenmiştir. Buna karşın, IRMangiferin grubunda over dokusunun histolojik bütünlüğünün korunduğu, folikül sayılarının daha yüksek olduğu ve AMH düzeylerinin göreceli olarak korunduğu saptanmıştır. Sonuç: Mangiferin, IR kaynaklı over hasarını azaltabilir ve over rezervinin korunmasına katkı sağlayabilir. Bulgular, Mangiferinin iskemik koşullarda over dokusunu korumaya yönelik potansiyel bir tedavi ajanı olabileceğini göstermektedir.

Makale detayları

Ovarian Ischemia-Reperfusion Injury and the Potential Role of Mangiferin: A Histopathological, Biochemical Investigation

Yazarlar:

Hayat Ayaz | Fırat Aşır

Yayıncı:

Kocaeli University

Ana Başlıklar:

Kadın Hastalıkları ve Doğum

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.11 Issue 3, pp.160-166

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359762

pISSN:

2149-8571

Anti Müllerian Hormone

Histopathology

İschemia Reperfusion İnjury

Mangiferin

OvaryAnti Müllerian Hormon

Histopatoloji

İskemi Reperfüzyon Hasarı

Mangiferin

Over

Sayı

Makale detayları

Yazarlar: Hayat Ayaz | Fırat Aşır

Yayıncı: Kocaeli University

Ana Başlıklar: Kadın Hastalıkları ve Doğum

Dergi: Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.11 Issue 3, pp.160-166

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359762

pISSN: 2149-8571

Sayı

Yazarlar:

Hayat Ayaz | Fırat Aşır

Yayıncı:

Kocaeli University

Ana Başlıklar:

Kadın Hastalıkları ve Doğum

Dergi:

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi Vol.11 Issue 3, pp.160-166

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359762

pISSN:

2149-8571