Bone marrow-derived mesenchymal stem cells mitigate colistin-induced cochlear ototoxicity: an experimental study

Özlem Öz Gergin; Sibel Seçkin Pehlivan; Adnan Bayram; Mustafa Birkin; Eda Köseoğlu; Demet Bolat; Esra Balcıoğlu

Makale detayları

Bone marrow-derived mesenchymal stem cells mitigate colistin-induced cochlear ototoxicity: an experimental study

Yazarlar:

Özlem Öz Gergin | Sibel Seçkin Pehlivan | Adnan Bayram | Mustafa Birkin | Eda Köseoğlu | Demet Bolat | Esra Balcıoğlu

Yayıncı:

Cukurova University

Ana Başlıklar:

Anesteziyoloji | Yoğun Bakım

Dergi:

Cukurova Medical Journal Vol.50 Issue 2, pp.287-298

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351452

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: This study investigates the therapeutic potential of mesenchymal stem cells in colistin-induced hearing loss. Materials and Methods: Forty Wistar albino rats were divided into four groups n10: Control 36 mgkgday intraperitoneal ip saline for 7 days, Colistin 36 mgkgday ip for 7 days, Mesenchymal stem cell group MSC 510⁶ MSC via tail vein, and ColistinMesenchymal stem cell group ColMSC colistin for 7 days, then MSC 1 hour after final dose. After the experiment, cochlear tissues were extracted, fixed, decalcified, embedded in paraffin, sectioned 5µm, and stained with Massons trichrome MT for histological evaluation under light microscopy. Results: Colistin caused marked structural damage, especially in the basal and medial cochlear turns. In the apical and medial regions, stria vascularis thickness was significantly lower in the colistin group 10.641.90, 11.542.32 than in the control 14.122.03, 15.432.26 and MSC 13.911.49, 14.531.80 groups. Significant intergroup differences were also noted in the basal turn. Inner hair cell lengths were similar apically but significantly reduced in the colistin 30.734.28 and ColMSC 31.066.96 groups medially. Outer hair cell lengths were significantly reduced in the colistin group in both apical 32.238.32 and basal 18.322.47 regions. Tectorial membrane thickness in the basal turn was significantly reduced in all treatment groups compared to the control 17.645.41. Based on Freitas et al.s criteria, the colistin group showed significantly higher histopathological damage scores in all regions compared to the control and MSC groups. The ColMSC group showed improved morphology and lower scores, though some differences lacked statistical significance. Conclusion: MSCs significantly alleviated colistin-induced ototoxicity, indicating their protective and therapeutic potential in inner ear injury.

 Purpose: This study investigates the therapeutic potential of mesenchymal stem cells in colistin-induced hearing loss. Materials and Methods: Forty Wistar albino rats were divided into four groups n10: Control 36 mgkgday intraperitoneal ip saline for 7 days, Colistin 36 mgkgday ip for 7 days, Mesenchymal stem cell group MSC 510⁶ MSC via tail vein, and ColistinMesenchymal stem cell group ColMSC colistin for 7 days, then MSC 1 hour after final dose. After the experiment, cochlear tissues were extracted, fixed, decalcified, embedded in paraffin, sectioned 5µm, and stained with Massons trichrome MT for histological evaluation under light microscopy. Results: Colistin caused marked structural damage, especially in the basal and medial cochlear turns. In the apical and medial regions, stria vascularis thickness was significantly lower in the colistin group 10.641.90, 11.542.32 than in the control 14.122.03, 15.432.26 and MSC 13.911.49, 14.531.80 groups. Significant intergroup differences were also noted in the basal turn. Inner hair cell lengths were similar apically but significantly reduced in the colistin 30.734.28 and ColMSC 31.066.96 groups medially. Outer hair cell lengths were significantly reduced in the colistin group in both apical 32.238.32 and basal 18.322.47 regions. Tectorial membrane thickness in the basal turn was significantly reduced in all treatment groups compared to the control 17.645.41. Based on Freitas et al.s criteria, the colistin group showed significantly higher histopathological damage scores in all regions compared to the control and MSC groups. The ColMSC group showed improved morphology and lower scores, though some differences lacked statistical significance. Conclusion: MSCs significantly alleviated colistin-induced ototoxicity, indicating their protective and therapeutic potential in inner ear injury.

 Purpose: This study investigates the therapeutic potential of mesenchymal stem cells in colistin-induced hearing loss. Materials and Methods: Forty Wistar albino rats were divided into four groups n10: Control 36 mgkgday intraperitoneal ip saline for 7 days, Colistin 36 mgkgday ip for 7 days, Mesenchymal stem cell group MSC 510⁶ MSC via tail vein, and ColistinMesenchymal stem cell group ColMSC colistin for 7 days, then MSC 1 hour after final dose. After the experiment, cochlear tissues were extracted, fixed, decalcified, embedded in paraffin, sectioned 5µm, and stained with Massons trichrome MT for histological evaluation under light microscopy. Results: Colistin caused marked structural damage, especially in the basal and medial cochlear turns. In the apical and medial regions, stria vascularis thickness was significantly lower in the colistin group 10.641.90, 11.542.32 than in the control 14.122.03, 15.432.26 and MSC 13.911.49, 14.531.80 groups. Significant intergroup differences were also noted in the basal turn. Inner hair cell lengths were similar apically but significantly reduced in the colistin 30.734.28 and ColMSC 31.066.96 groups medially. Outer hair cell lengths were significantly reduced in the colistin group in both apical 32.238.32 and basal 18.322.47 regions. Tectorial membrane thickness in the basal turn was significantly reduced in all treatment groups compared to the control 17.645.41. Based on Freitas et al.s criteria, the colistin group showed significantly higher histopathological damage scores in all regions compared to the control and MSC groups. The ColMSC group showed improved morphology and lower scores, though some differences lacked statistical significance. Conclusion: MSCs significantly alleviated colistin-induced ototoxicity, indicating their protective and therapeutic potential in inner ear injury.

 Amaç: Bu çalışma, kolistin kaynaklı işitme kaybında mezenkimal kök hücrelerin MSC terapötik potansiyelini araştırmayı amaçlamaktadır. Gereç ve Yöntem: Kırk Wistar albino sıçan dört gruba ayrıldı n10: Kontrol 7 gün boyunca 36 mgkggün intraperitoneal ip serum fizyolojik, Kolistin 7 gün boyunca 36 mgkggün ip kolistin, Mezenkimal kök hücre grubu MSC kuyruk veninden 510⁶ MSC, ve KolistinMSC grubu ColMSC 7 gün boyunca kolistin, son dozdan 1 saat sonra 510⁶ MSC. Deney sonunda, koklea dokuları çıkarıldı, fikse edildi, dekalcifiye edildi, parafin bloklara gömüldü, 5 µm kalınlığında kesitler alındı ve Massons trikrom MT ile boyanarak ışık mikroskobu altında histolojik olarak değerlendirildi. Bulgular: Kolistin grubu özellikle kokleanın bazal ve medial kıvrımlarında belirgin yapısal hasar gösterdi. Apikal ve medial bölgelerde, stria vaskülaris kalınlığı kolistin grubunda 10.641.90, 11.542.32 kontrol 14.122.03, 15.432.26 ve MSC 13.911.49, 14.531.80 gruplarına göre anlamlı derecede azdı. Bazal kıvrımda da gruplar arasında anlamlı farklılıklar gözlendi. İç tüylü hücrelerin uzunlukları apikalde benzerken, medialde kolistin 30.734.28 ve ColMSC 31.066.96 gruplarında anlamlı azalma görüldü. Dış tüylü hücre uzunlukları ise kolistin grubunda hem apikal 32.238.32 hem de bazal 18.322.47 bölgelerde anlamlı şekilde kısaydı. Bazal kıvrımda, tectorial membran kalınlığı tüm tedavi gruplarında kontrol grubuna 17.645.41 kıyasla anlamlı şekilde azaldı. Freitas ve ark. tarafından tanımlanan kriterlere göre, kolistin grubunun tüm bölgelerde histopatolojik hasar skorları kontrol ve MSC gruplarına göre anlamlı olarak daha yüksekti. ColMSC grubu daha organize morfoloji ve düşük skorlar gösterse de bazı farklılıklar istatistiksel olarak anlamlı değildi. Sonuç: MSCler, kolistin kaynaklı ototoksisiteyi anlamlı derecede hafifleterek iç kulak hasarında koruyucu ve terapötik potansiyele sahip olduklarını göstermektedir.

Makale detayları

Bone marrow-derived mesenchymal stem cells mitigate colistin-induced cochlear ototoxicity: an experimental study

Yazarlar:

Özlem Öz Gergin | Sibel Seçkin Pehlivan | Adnan Bayram | Mustafa Birkin | Eda Köseoğlu | Demet Bolat | Esra Balcıoğlu

Yayıncı:

Cukurova University

Ana Başlıklar:

Anesteziyoloji | Yoğun Bakım

Dergi:

Cukurova Medical Journal Vol.50 Issue 2, pp.287-298

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351452

pISSN:

2602-3032

Colistin;

ototoxicity,

mesenchymal stem cell,

cochlea,

organ of cortiKolistin,

ototoksisite,

mezenkimal kök hücre,

koklea,

korti organı,

Sayı

Makale detayları

Yazarlar: Özlem Öz Gergin | Sibel Seçkin Pehlivan | Adnan Bayram | Mustafa Birkin | Eda Köseoğlu | Demet Bolat | Esra Balcıoğlu

Yayıncı: Cukurova University

Ana Başlıklar: Anesteziyoloji | Yoğun Bakım

Dergi: Cukurova Medical Journal Vol.50 Issue 2, pp.287-298

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001351452

pISSN: 2602-3032

Sayı

Yazarlar:

Özlem Öz Gergin | Sibel Seçkin Pehlivan | Adnan Bayram | Mustafa Birkin | Eda Köseoğlu | Demet Bolat | Esra Balcıoğlu

Yayıncı:

Cukurova University

Ana Başlıklar:

Anesteziyoloji | Yoğun Bakım

Dergi:

Cukurova Medical Journal Vol.50 Issue 2, pp.287-298

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351452

pISSN:

2602-3032