Efficacy of Some Disinfectants on Embryonated Eggs of Toxocara canis

Hasan AYÇİÇEK

Makale detayları

Efficacy of Some Disinfectants on Embryonated Eggs of Toxocara canis

Yazarlar:

Hasan AYÇİÇEK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2001 Vol.31 Issue 1, pp.35-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757417

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The aim of this study was to evaluate the efficacy of various disinfectants in different concentrations against embryonated Toxocara canis eggs. For this purpose, 2.5%, 5%, 7.5%, and 10% iodine, 2% glutaraldehyde, 10% benzalkonium chloride, 7% sodium hypochloride, 1% potassium permanganate, 70% ethyl alcohol, 10% potassium hydroxide and 3% phenol solutions were used as the disinfectants. This study was performed both in vitro and in-vivo. In the in vitro experiment, T. canis eggs were treated with disinfectant solutions in different time intervals, and larval motility was observed under X400 magnification with an inverted microscope. Microscopic examinations revealed that T. canis eggs treated in 2.5%, 5%, 7.5%, and 10% iodine solutions were completely non-motile at 120, 60, 40, and 40 minutes post-treatment respectively, whereas the eggs treated in the all other disinfectants were still motile after 24 hours. In the in-vivo study, 1000 embryonated eggs treated with disinfectants were inoculated each mouse via the oral route, and their brain tissues were examined for larval presence on the 7th day post-inoculation. In addition, a control group was set up for comparison with the study groups. No T. canis larvae were observed in mice inoculated with eggs treated with any of the iodine solutions. However, larvae were observed in the other study groups inoculated with eggs treated with the other disinfectants.  These results showed that only the iodine disinfectants produced a statistically significant difference  (p.

 

The aim of this study was to evaluate the efficacy of various disinfectants in different concentrations against embryonated Toxocara canis eggs. For this purpose, 2.5%, 5%, 7.5%, and 10% iodine, 2% glutaraldehyde, 10% benzalkonium chloride, 7% sodium hypochloride, 1% potassium permanganate, 70% ethyl alcohol, 10% potassium hydroxide and 3% phenol solutions were used as the disinfectants. This study was performed both in vitro and in-vivo. In the in vitro experiment, T. canis eggs were treated with disinfectant solutions in different time intervals, and larval motility was observed under X400 magnification with an inverted microscope. Microscopic examinations revealed that T. canis eggs treated in 2.5%, 5%, 7.5%, and 10% iodine solutions were completely non-motile at 120, 60, 40, and 40 minutes post-treatment respectively, whereas the eggs treated in the all other disinfectants were still motile after 24 hours. In the in-vivo study, 1000 embryonated eggs treated with disinfectants were inoculated each mouse via the oral route, and their brain tissues were examined for larval presence on the 7th day post-inoculation. In addition, a control group was set up for comparison with the study groups. No T. canis larvae were observed in mice inoculated with eggs treated with any of the iodine solutions. However, larvae were observed in the other study groups inoculated with eggs treated with the other disinfectants.  These results showed that only the iodine disinfectants produced a statistically significant difference  (p.

Makale detayları

Efficacy of Some Disinfectants on Embryonated Eggs of Toxocara canis

Yazarlar:

Hasan AYÇİÇEK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2001 Vol.31 Issue 1, pp.35-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757417

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Toxocara canis

disinfectants

efficiency

Sayı

Makale detayları

Efficacy of Some Disinfectants on Embryonated Eggs of Toxocara canis

Yazarlar: Hasan AYÇİÇEK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2001 Vol.31 Issue 1, pp.35-39

Belge Tipi: Makale

Yayın Tarihi: 12/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000757417

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Toxocara canis

disinfectants

efficiency

Sayı

Yazarlar:

Hasan AYÇİÇEK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2001 Vol.31 Issue 1, pp.35-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757417

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144