Investigation of Mobilized Colistin Resistance Gene-1 in Poultry Pathogenic  Escherichia coli

Süheyla Türkyılmaz

Makale detayları

Investigation of Mobilized Colistin Resistance Gene-1 in Poultry Pathogenic Escherichia coli

Yazarlar:

Süheyla Türkyılmaz

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Animal Health Production and Hygiene Vol.13 Issue 2, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001336689

pISSN:

2146-7269

Anahtar kelimeler:

Özet:

 This study aimed to determine the presence of mobilized colistin resistance gene 1 mcr-1, which provides plasmid-mediated colistin resistance in avian pathogenic Escherichia coli APEC isolates, and to examine the antibiotic resistance profiles of colistin resistant isolates. In this research, 200 broiler liver samples suspected of colibacillosis were utilized as the material. Following the isolation of E. coli through classical conventional methods, identification and antibiotic susceptibility tests were conducted using an automated microbiology system BD Phoenix 100TM, USA. The presence of the mcr-1 gene in isolates phenotypically determined as colistin resistant was investigated using polymerase chain reaction PCR. Out of 156 78 E. coli isolates obtained from 200 broiler liver samples, 10 6.4 were phenotypically determined to be colistin resistant. It was found that 80 of colistin resistant E. coli isolates were resistant to levofloxacin, 70 to cefazolin and cefuroxime, 60 to ceftazidime, 50 to gentamicin and ceftriaxone, 40 to cefepime, 30 to ceftolozane-tazobactam, and 20 to piperacillin-tazobactam. All isolates were sensitive to amikacin, ertapenem, imipenem, meropenem, and resistant to ampicillin, amoxicillin-clavulanate, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, and tigecycline, exhibiting multidrug resistance MDR. All isolates that were found to be phenotypically resistant to colistin were found to carry the mcr-1 gene. These findings indicate that the mcr in APEC contributes to the rapid spread of plasmid-mediated resistance genes and the escalation of broad-spectrum antibiotic resistance. The detection of resistance to antibiotics used in human medicine may be poses a potential threat to public health. Future studies should be conducted with samples from different regions and include a diverse sample group to better understand this risk.

 This study aimed to determine the presence of mobilized colistin resistance gene 1 mcr-1, which provides plasmid-mediated colistin resistance in avian pathogenic Escherichia coli APEC isolates, and to examine the antibiotic resistance profiles of colistin resistant isolates. In this research, 200 broiler liver samples suspected of colibacillosis were utilized as the material. Following the isolation of E. coli through classical conventional methods, identification and antibiotic susceptibility tests were conducted using an automated microbiology system BD Phoenix 100TM, USA. The presence of the mcr-1 gene in isolates phenotypically determined as colistin resistant was investigated using polymerase chain reaction PCR. Out of 156 78 E. coli isolates obtained from 200 broiler liver samples, 10 6.4 were phenotypically determined to be colistin resistant. It was found that 80 of colistin resistant E. coli isolates were resistant to levofloxacin, 70 to cefazolin and cefuroxime, 60 to ceftazidime, 50 to gentamicin and ceftriaxone, 40 to cefepime, 30 to ceftolozane-tazobactam, and 20 to piperacillin-tazobactam. All isolates were sensitive to amikacin, ertapenem, imipenem, meropenem, and resistant to ampicillin, amoxicillin-clavulanate, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, and tigecycline, exhibiting multidrug resistance MDR. All isolates that were found to be phenotypically resistant to colistin were found to carry the mcr-1 gene. These findings indicate that the mcr in APEC contributes to the rapid spread of plasmid-mediated resistance genes and the escalation of broad-spectrum antibiotic resistance. The detection of resistance to antibiotics used in human medicine may be poses a potential threat to public health. Future studies should be conducted with samples from different regions and include a diverse sample group to better understand this risk.

 This study aimed to determine the presence of mobilized colistin resistance gene 1 mcr-1, which provides plasmid-mediated colistin resistance in avian pathogenic Escherichia coli APEC isolates, and to examine the antibiotic resistance profiles of colistin resistant isolates. In this research, 200 broiler liver samples suspected of colibacillosis were utilized as the material. Following the isolation of E. coli through classical conventional methods, identification and antibiotic susceptibility tests were conducted using an automated microbiology system BD Phoenix 100TM, USA. The presence of the mcr-1 gene in isolates phenotypically determined as colistin resistant was investigated using polymerase chain reaction PCR. Out of 156 78 E. coli isolates obtained from 200 broiler liver samples, 10 6.4 were phenotypically determined to be colistin resistant. It was found that 80 of colistin resistant E. coli isolates were resistant to levofloxacin, 70 to cefazolin and cefuroxime, 60 to ceftazidime, 50 to gentamicin and ceftriaxone, 40 to cefepime, 30 to ceftolozane-tazobactam, and 20 to piperacillin-tazobactam. All isolates were sensitive to amikacin, ertapenem, imipenem, meropenem, and resistant to ampicillin, amoxicillin-clavulanate, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, and tigecycline, exhibiting multidrug resistance MDR. All isolates that were found to be phenotypically resistant to colistin were found to carry the mcr-1 gene. These findings indicate that the mcr in APEC contributes to the rapid spread of plasmid-mediated resistance genes and the escalation of broad-spectrum antibiotic resistance. The detection of resistance to antibiotics used in human medicine may be poses a potential threat to public health. Future studies should be conducted with samples from different regions and include a diverse sample group to better understand this risk.

 This study aimed to determine the presence of mobilized colistin resistance gene 1 mcr-1, which provides plasmid-mediated colistin resistance in avian pathogenic Escherichia coli APEC isolates, and to examine the antibiotic resistance profiles of colistin resistant isolates. In this research, 200 broiler liver samples suspected of colibacillosis were utilized as the material. Following the isolation of E. coli through classical conventional methods, identification and antibiotic susceptibility tests were conducted using an automated microbiology system BD Phoenix 100TM, USA. The presence of the mcr-1 gene in isolates phenotypically determined as colistin resistant was investigated using polymerase chain reaction PCR. Out of 156 78 E. coli isolates obtained from 200 broiler liver samples, 10 6.4 were phenotypically determined to be colistin resistant. It was found that 80 of colistin resistant E. coli isolates were resistant to levofloxacin, 70 to cefazolin and cefuroxime, 60 to ceftazidime, 50 to gentamicin and ceftriaxone, 40 to cefepime, 30 to ceftolozane-tazobactam, and 20 to piperacillin-tazobactam. All isolates were sensitive to amikacin, ertapenem, imipenem, meropenem, and resistant to ampicillin, amoxicillin-clavulanate, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, and tigecycline, exhibiting multidrug resistance MDR. All isolates that were found to be phenotypically resistant to colistin were found to carry the mcr-1 gene. These findings indicate that the mcr in APEC contributes to the rapid spread of plasmid-mediated resistance genes and the escalation of broad-spectrum antibiotic resistance. The detection of resistance to antibiotics used in human medicine may be poses a potential threat to public health. Future studies should be conducted with samples from different regions and include a diverse sample group to better understand this risk.

Makale detayları

Investigation of Mobilized Colistin Resistance Gene-1 in Poultry Pathogenic Escherichia coli

Yazarlar:

Süheyla Türkyılmaz

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Animal Health Production and Hygiene Vol.13 Issue 2, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001336689

pISSN:

2146-7269

Avian pathogenic Escherichia coli

mobile colistin resistance gene

antibiotic resistance

Sayı

Makale detayları

Investigation of Mobilized Colistin Resistance Gene-1 in Poultry Pathogenic Escherichia coli

Yazarlar: Süheyla Türkyılmaz

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Veteriner Bilimleri (Diğer)

Dergi: Animal Health Production and Hygiene Vol.13 Issue 2, pp.1-8

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001336689

pISSN: 2146-7269

Avian pathogenic Escherichia coli

mobile colistin resistance gene

antibiotic resistance

Sayı

Yazarlar:

Süheyla Türkyılmaz

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Animal Health Production and Hygiene Vol.13 Issue 2, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001336689

pISSN:

2146-7269