Determination of Serum and Urine Proteins by Electrophoresis and Its Importance in Clinical Veterinary Medicine

Arif ALTINTAŞ

Makale detayları

Determination of Serum and Urine Proteins by Electrophoresis and Its Importance in Clinical Veterinary Medicine

Yazarlar:

Arif ALTINTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.93-104

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771510

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

In this study, for the investigation of routine clinical usage of urine protein electrophoresis in the laboratory to compare  with the clinical findings in veterinary medicine, qualitative and quantitative changes in protein patterns of serum and urine from  dogs with kidney disorders and proteinuria were investigated using electrophoresis.  Eighteen dogs (patient group)of various ages and breeds with kidney disorders proved in the clinic and the laboratory and seventeen  healthy dogs (control group)were used in this study. The histories (age,sex,breed)of the dogs in the control and patient groups  were recorded in the clinic.The blood was collected from the cephalic vein,and the serum was removed using a centrifuge.Urine  was obtained by a sterile catheter from the ureter.Biochemical analysis of urine was performed in the clinic.The urines of dogs with  proteinuria were centrifuged and ultrafiltrated to concentrate for protein electrophoresis. Serum and urine proteins were separated  by 10 %SDS-PAGE. Creatinine in serum and urine was detected using the Jaffe reaction, and urea and total protein in serum by  the modified Gentzkow and biuret methods respectively. A/G ratios in serum and protein/creatinine ratios in urine were calculated  arithmetically.  Levels of serum urea and creatinine were found to be high and statistically significant (p.

 

In this study, for the investigation of routine clinical usage of urine protein electrophoresis in the laboratory to compare  with the clinical findings in veterinary medicine, qualitative and quantitative changes in protein patterns of serum and urine from  dogs with kidney disorders and proteinuria were investigated using electrophoresis.  Eighteen dogs (patient group)of various ages and breeds with kidney disorders proved in the clinic and the laboratory and seventeen  healthy dogs (control group)were used in this study. The histories (age,sex,breed)of the dogs in the control and patient groups  were recorded in the clinic.The blood was collected from the cephalic vein,and the serum was removed using a centrifuge.Urine  was obtained by a sterile catheter from the ureter.Biochemical analysis of urine was performed in the clinic.The urines of dogs with  proteinuria were centrifuged and ultrafiltrated to concentrate for protein electrophoresis. Serum and urine proteins were separated  by 10 %SDS-PAGE. Creatinine in serum and urine was detected using the Jaffe reaction, and urea and total protein in serum by  the modified Gentzkow and biuret methods respectively. A/G ratios in serum and protein/creatinine ratios in urine were calculated  arithmetically.  Levels of serum urea and creatinine were found to be high and statistically significant (p.

Makale detayları

Determination of Serum and Urine Proteins by Electrophoresis and Its Importance in Clinical Veterinary Medicine

Yazarlar:

Arif ALTINTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.93-104

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771510

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Dog

Kidney disorder

Proteinuria

SDS-PAGE

Urine

Serum.

Sayı

Makale detayları

Determination of Serum and Urine Proteins by Electrophoresis and Its Importance in Clinical Veterinary Medicine

Yazarlar: Arif ALTINTAŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.93-104

Belge Tipi: Makale

Yayın Tarihi: 12/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000771510

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Dog

Kidney disorder

Proteinuria

SDS-PAGE

Urine

Serum.

Sayı

Yazarlar:

Arif ALTINTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.93-104

Belge Tipi:

Makale

Yayın Tarihi:

12/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771510

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128