In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

Hilal Basak Erol; Banu Kaşkatepe

Makale detayları

In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

Yazarlar:

Hilal Basak Erol | Banu Kaşkatepe

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Microbiology

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.133-141

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345987

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Salmonella is an important agent that causes foodborne gastrointestinal infections. Salmonella enterica serovar Enteritidis, serovar Typhimurium, and serovar Infantis are the main pathogenic agents of several enteric infections worldwide. Due to the emergence of multidrug resistant Salmonella strains, there is a need for new administration strategies alternative to antibiotics, such as bacteriophages, for effective control of them. The main objectives of this study were to isolate and characterize Salmonella spp. bacteriophage and compare its activity to commercial phage product and assess the effect of bacteriophages in vitro during 24-h. Because of the study, it was found that the newly isolated and named vB_SiM_12 phage could lyse 100 of the strains tested. Bacterial growth with dilutions of vB_SiM_12 phage MOI ranging from 0.001 to 10 was measured during 24-h. The optic density values of the samples with added bacteriophage remained lower than the control sample during the 24-h incubation period. The results show that using phages may offer a promising alternative to combat biological control agents against Salmonella infection.

 Salmonella is an important agent that causes foodborne gastrointestinal infections. Salmonella enterica serovar Enteritidis, serovar Typhimurium, and serovar Infantis are the main pathogenic agents of several enteric infections worldwide. Due to the emergence of multidrug resistant Salmonella strains, there is a need for new administration strategies alternative to antibiotics, such as bacteriophages, for effective control of them. The main objectives of this study were to isolate and characterize Salmonella spp. bacteriophage and compare its activity to commercial phage product and assess the effect of bacteriophages in vitro during 24-h. Because of the study, it was found that the newly isolated and named vB_SiM_12 phage could lyse 100 of the strains tested. Bacterial growth with dilutions of vB_SiM_12 phage MOI ranging from 0.001 to 10 was measured during 24-h. The optic density values of the samples with added bacteriophage remained lower than the control sample during the 24-h incubation period. The results show that using phages may offer a promising alternative to combat biological control agents against Salmonella infection.

 Salmonella is an important agent that causes foodborne gastrointestinal infections. Salmonella enterica serovar Enteritidis, serovar Typhimurium, and serovar Infantis are the main pathogenic agents of several enteric infections worldwide. Due to the emergence of multidrug resistant Salmonella strains, there is a need for new administration strategies alternative to antibiotics, such as bacteriophages, for effective control of them. The main objectives of this study were to isolate and characterize Salmonella spp. bacteriophage and compare its activity to commercial phage product and assess the effect of bacteriophages in vitro during 24-h. Because of the study, it was found that the newly isolated and named vB_SiM_12 phage could lyse 100 of the strains tested. Bacterial growth with dilutions of vB_SiM_12 phage MOI ranging from 0.001 to 10 was measured during 24-h. The optic density values of the samples with added bacteriophage remained lower than the control sample during the 24-h incubation period. The results show that using phages may offer a promising alternative to combat biological control agents against Salmonella infection.

 Salmonella is an important agent that causes foodborne gastrointestinal infections. Salmonella enterica serovar Enteritidis, serovar Typhimurium, and serovar Infantis are the main pathogenic agents of several enteric infections worldwide. Due to the emergence of multidrug resistant Salmonella strains, there is a need for new administration strategies alternative to antibiotics, such as bacteriophages, for effective control of them. The main objectives of this study were to isolate and characterize Salmonella spp. bacteriophage and compare its activity to commercial phage product and assess the effect of bacteriophages in vitro during 24-h. Because of the study, it was found that the newly isolated and named vB_SiM_12 phage could lyse 100 of the strains tested. Bacterial growth with dilutions of vB_SiM_12 phage MOI ranging from 0.001 to 10 was measured during 24-h. The optic density values of the samples with added bacteriophage remained lower than the control sample during the 24-h incubation period. The results show that using phages may offer a promising alternative to combat biological control agents against Salmonella infection.

Makale detayları

In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

Yazarlar:

Hilal Basak Erol | Banu Kaşkatepe

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Microbiology

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.133-141

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345987

pISSN:

2630-6344

Bacteriophage

bacteriolytic activity

invitro turbidity test

Salmonella spp.

Sayı

Makale detayları

In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

Yazarlar: Hilal Basak Erol | Banu Kaşkatepe

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Microbiology

Dergi: Journal of Research in Pharmacy Vol.28 Issue 1, pp.133-141

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001345987

pISSN: 2630-6344

Bacteriophage

bacteriolytic activity

invitro turbidity test

Salmonella spp.

Sayı

Yazarlar:

Hilal Basak Erol | Banu Kaşkatepe

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Microbiology

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.133-141

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345987

pISSN:

2630-6344