Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran

Elham AHMADI; Seyed Ali POURBAKHSH; Malahat AHMADI; Alireza TALEBI

Makale detayları

Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran

Yazarlar:

Elham AHMADI | Seyed Ali POURBAKHSH | Malahat AHMADI | Alireza TALEBI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 4, pp.383-387

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000765924

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Despite vaccination, outbreaks of Newcastle disease in commercial poultry flocks in northwest Iran have been regularly reported in recent years. This research was proposed in order to characterize, through a molecular approach, the genetic nature of strains of Newcastle disease virus isolated from commercial poultry flocks in northwest Iran. Ten Newcastle disease virus isolates were obtained from various districts of northwest Iran in 2010. The isolates were screened with F gene-targeting reverse transcription polymerase chain reaction (RT-PCR). The amplified products of virulent strains were sequenced. The deduced amino acid sequence of the fusion protein cleavage site indicated the presence of motif 112R-R-Q-R-R.F117, typical for velogenic strains of NDV. These results confirmed that virulent NDVs are circulating in the region, causing economic losses within the poultry industry.

 

Despite vaccination, outbreaks of Newcastle disease in commercial poultry flocks in northwest Iran have been regularly reported in recent years. This research was proposed in order to characterize, through a molecular approach, the genetic nature of strains of Newcastle disease virus isolated from commercial poultry flocks in northwest Iran. Ten Newcastle disease virus isolates were obtained from various districts of northwest Iran in 2010. The isolates were screened with F gene-targeting reverse transcription polymerase chain reaction (RT-PCR). The amplified products of virulent strains were sequenced. The deduced amino acid sequence of the fusion protein cleavage site indicated the presence of motif 112R-R-Q-R-R.F117, typical for velogenic strains of NDV. These results confirmed that virulent NDVs are circulating in the region, causing economic losses within the poultry industry.

Makale detayları

Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran

Yazarlar:

Elham AHMADI | Seyed Ali POURBAKHSH | Malahat AHMADI | Alireza TALEBI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 4, pp.383-387

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000765924

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Newcastle disease virus

northwest Iran

fusion protein

cleavage site

RT-PCR

sequencing

Sayı

Makale detayları

Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran

Yazarlar: Elham AHMADI | Seyed Ali POURBAKHSH | Malahat AHMADI | Alireza TALEBI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 4, pp.383-387

Belge Tipi: Makale

Yayın Tarihi: 3/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000765924

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Newcastle disease virus

northwest Iran

fusion protein

cleavage site

RT-PCR

sequencing

Sayı

Yazarlar:

Elham AHMADI | Seyed Ali POURBAKHSH | Malahat AHMADI | Alireza TALEBI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 4, pp.383-387

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000765924

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128