Investigation of Cytotoxic Effects of Boron Compounds Containing Chiral Group as Bioactive Agents in A549 Lung Cancer Cells

Nilgün Okşak; Ahmet Kılıç

Makale detayları

Investigation of Cytotoxic Effects of Boron Compounds Containing Chiral Group as Bioactive Agents in A549 Lung Cancer Cells

Yazarlar:

Nilgün Okşak | Ahmet Kılıç

Yayıncı:

Harran University

Ana Başlıklar:

Tıbbi Farmakoloji | Eczacılık ve İlaç Bilimleri (Diğer) | Kemoterapi

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.22 Issue 3, pp.594-600

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352404

pISSN:

1304-9623

Anahtar kelimeler:

Özet:

 Background: In lung cancer, which progresses insidiously and is diagnosed at a late stage, recurrence of the disease, development of drug resistance or limited efficacy of conventional treatment remains a problem. These treatment chal-lenging factors lead researchers to new researchs; recently, boron-containing compounds have attracted interest due to their cytotoxic or genotoxic effects in cancer types. Therefore, the aim of this study was to investigate for the first time the cytotoxic effect of chiral group boron-based compounds in human alveolar cell adenocarcinoma A549 cell line. Materials and Methods: Compounds T1B1 and T1B2 were re-synthesized in the Dean-Stark system under suitable reaction conditions by modifying some stoichiometric ratios based on the previously described methods. The cytotoxic effects of T1B1 and T1B2, three coordinated boron compounds containing chiral groups as bioactive agents in A549 adenocarcinoma cells, were determined calorimetrically by the MTT method. For this purpose, the synthesized compounds were used at concen-trations of 10-60 µgml and incubated with A549 cells for 48 and 72 hours. Then, the IC50 value of each compound was calculated using the optical density OD data obtained. Results: According to the results obtained, cytotoxic effects of three coordinated T1B1 and T1B2 boron compounds containing chiral groups as bioactive agents studied in the concentration range of 10-60 µgml were determined on A549 cell line. It was determined that significant cytotoxic effect occurred at all concentrations of both compounds compared to the control group p0.001. The IC50 values for compound T1B1 were calculated as 15.84 µgml at 48 h and 12.22 µgml at 72 h, while for compound T1B2, the IC50 values were 19.62 µgml at 48 h and 13.59 µgml at 72 h. This indicated that the cytotoxic activity of T1B1 was stronger than that of boron compounds. Conclusions: It was determined that T1B1 and T1B2 boron compounds applied to A549 adenocarcinoma cells decreased proliferation and produced a cytotoxic effect, depending on increasing concentration and time. Further elucidation of the biochemical and molecular mechanisms of newly synthesized boron compounds will become important in terms of deter-mining their role in cancer prevention and treatment.

 Background: In lung cancer, which progresses insidiously and is diagnosed at a late stage, recurrence of the disease, development of drug resistance or limited efficacy of conventional treatment remains a problem. These treatment chal-lenging factors lead researchers to new researchs; recently, boron-containing compounds have attracted interest due to their cytotoxic or genotoxic effects in cancer types. Therefore, the aim of this study was to investigate for the first time the cytotoxic effect of chiral group boron-based compounds in human alveolar cell adenocarcinoma A549 cell line. Materials and Methods: Compounds T1B1 and T1B2 were re-synthesized in the Dean-Stark system under suitable reaction conditions by modifying some stoichiometric ratios based on the previously described methods. The cytotoxic effects of T1B1 and T1B2, three coordinated boron compounds containing chiral groups as bioactive agents in A549 adenocarcinoma cells, were determined calorimetrically by the MTT method. For this purpose, the synthesized compounds were used at concen-trations of 10-60 µgml and incubated with A549 cells for 48 and 72 hours. Then, the IC50 value of each compound was calculated using the optical density OD data obtained. Results: According to the results obtained, cytotoxic effects of three coordinated T1B1 and T1B2 boron compounds containing chiral groups as bioactive agents studied in the concentration range of 10-60 µgml were determined on A549 cell line. It was determined that significant cytotoxic effect occurred at all concentrations of both compounds compared to the control group p0.001. The IC50 values for compound T1B1 were calculated as 15.84 µgml at 48 h and 12.22 µgml at 72 h, while for compound T1B2, the IC50 values were 19.62 µgml at 48 h and 13.59 µgml at 72 h. This indicated that the cytotoxic activity of T1B1 was stronger than that of boron compounds. Conclusions: It was determined that T1B1 and T1B2 boron compounds applied to A549 adenocarcinoma cells decreased proliferation and produced a cytotoxic effect, depending on increasing concentration and time. Further elucidation of the biochemical and molecular mechanisms of newly synthesized boron compounds will become important in terms of deter-mining their role in cancer prevention and treatment.

 Background: In lung cancer, which progresses insidiously and is diagnosed at a late stage, recurrence of the disease, development of drug resistance or limited efficacy of conventional treatment remains a problem. These treatment chal-lenging factors lead researchers to new researchs; recently, boron-containing compounds have attracted interest due to their cytotoxic or genotoxic effects in cancer types. Therefore, the aim of this study was to investigate for the first time the cytotoxic effect of chiral group boron-based compounds in human alveolar cell adenocarcinoma A549 cell line. Materials and Methods: Compounds T1B1 and T1B2 were re-synthesized in the Dean-Stark system under suitable reaction conditions by modifying some stoichiometric ratios based on the previously described methods. The cytotoxic effects of T1B1 and T1B2, three coordinated boron compounds containing chiral groups as bioactive agents in A549 adenocarcinoma cells, were determined calorimetrically by the MTT method. For this purpose, the synthesized compounds were used at concen-trations of 10-60 µgml and incubated with A549 cells for 48 and 72 hours. Then, the IC50 value of each compound was calculated using the optical density OD data obtained. Results: According to the results obtained, cytotoxic effects of three coordinated T1B1 and T1B2 boron compounds containing chiral groups as bioactive agents studied in the concentration range of 10-60 µgml were determined on A549 cell line. It was determined that significant cytotoxic effect occurred at all concentrations of both compounds compared to the control group p0.001. The IC50 values for compound T1B1 were calculated as 15.84 µgml at 48 h and 12.22 µgml at 72 h, while for compound T1B2, the IC50 values were 19.62 µgml at 48 h and 13.59 µgml at 72 h. This indicated that the cytotoxic activity of T1B1 was stronger than that of boron compounds. Conclusions: It was determined that T1B1 and T1B2 boron compounds applied to A549 adenocarcinoma cells decreased proliferation and produced a cytotoxic effect, depending on increasing concentration and time. Further elucidation of the biochemical and molecular mechanisms of newly synthesized boron compounds will become important in terms of deter-mining their role in cancer prevention and treatment.

 Amaç: Sinsi şekilde ilerleyerek geç evrede teşhis edilen akciğer kanserinde hastalığın nüksetmesi, ilaç direncinin gelişmesi veya klasik tedavi etkinliğinin sınırlı olması hala bir sorun olmaya devam etmektedir. Tedaviyi zorlayan bu faktörler araştırmacıları yeni arayışlara yönlendirmektedir ki; son zamanlarda bor içeren bileşikler, kanser türlerinde sitotoksik veya genotoksik etkilerinden dolayı ilgi çekmektedir. Bu nedenle çalışmada ilk kez kiral grubu bor temelli bileşiklerin insan alveolar hücre adenokarsinomu olan A549 hücre hattında sitotoksik etkisinin incelenmesi amaçlandı. Materyal ve metod: T1B1 ve T1B2 bileşikleri daha önce açıklanan yöntemler üzerinde bazı stokiyometrik oranlarda değişik-likler yapılarak Dean-Stark sisteminde uygun reaksiyon koşullarda yeniden sentezlendi. Sentezlenen T1B1 ve T1B2 bileşikle-ri, 10-60 µgml konsantrasyonlarda 6 dozda A549 hücrelere 48 ve 72 saat ayrı ayrı uygulandı. A549 adenokarsinoma hücrelerinde kiral grup içeren üç koordineli bor bileşikleri olan T1B1 ve T1B2nin sitotoksik etkileri kolorimetrik olarak MTT yöntemiyle belirlendi. Ardından elde edilen optik dansite OD verilerinden yararlanılarak her bileşiğe ait IC50 değeri hesap-landı. Bulgular: Elde edilen sonuçlara göre 10-60 µgml konsantrasyon aralığında çalışılan biyoaktif ajan olarak kiral grup içeren üç koordineli T1B1 ve T1B2 bor bileşikleri A549 hücre hattı üzerinde sitotoksik etkileri tespit edildi. Kontrol grubuna göre her iki bileşiğin tüm konsantrasyonlarında önemli düzeyde sitotoksik etki oluştuğu belirlendi p0.001. IC50 değerlerine baktı-ğımızda, T1B1 bileşiği için 48 saatte 15.84 µgml ve 72 saatte 12.22 µgml olarak, T1B2 bileşiği için 48 saatte 19.62 µgml ve 72 saatte 13.59 µgml olarak hesaplandı. Bu bor bileşiklerden T1B1in sitotoksik aktivitesinin daha güçlü olduğu belirlendi. Sonuç: A549 hücrelere uygulanan T1B1 ve T1B2 bor bileşiklerin artan zamana ve konsantrasyonlara bağlı olarak proliferas-yonu azalttığı ve sitotoksik etki oluşturduğunu belirledik. Gelecekte yeni sentezlenecek bor bileşiklerin kanser hücrelerin-deki biyokimyasal ve moleküler mekanizmalar üzerinde etki mekanizmalarının daha detaylı açıklanması kanserle mücade-lede ve tedavisindeki rolünün belirlenmesi açısından önemli olacaktır.

Makale detayları

Investigation of Cytotoxic Effects of Boron Compounds Containing Chiral Group as Bioactive Agents in A549 Lung Cancer Cells

Yazarlar:

Nilgün Okşak | Ahmet Kılıç

Yayıncı:

Harran University

Ana Başlıklar:

Tıbbi Farmakoloji | Eczacılık ve İlaç Bilimleri (Diğer) | Kemoterapi

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.22 Issue 3, pp.594-600

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352404

pISSN:

1304-9623

A549 cell

anticancer

boron compoundsA549 hücre

antikanser

bor bileşikleri

Sayı

Makale detayları

Investigation of Cytotoxic Effects of Boron Compounds Containing Chiral Group as Bioactive Agents in A549 Lung Cancer Cells

Yazarlar: Nilgün Okşak | Ahmet Kılıç

Yayıncı: Harran University

Ana Başlıklar: Tıbbi Farmakoloji | Eczacılık ve İlaç Bilimleri (Diğer) | Kemoterapi

Dergi: Harran Üniversitesi Tıp Fakültesi Dergisi Vol.22 Issue 3, pp.594-600

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001352404

pISSN: 1304-9623

A549 cell

anticancer

boron compoundsA549 hücre

antikanser

bor bileşikleri

Sayı

Yazarlar:

Nilgün Okşak | Ahmet Kılıç

Yayıncı:

Harran University

Ana Başlıklar:

Tıbbi Farmakoloji | Eczacılık ve İlaç Bilimleri (Diğer) | Kemoterapi

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.22 Issue 3, pp.594-600

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352404

pISSN:

1304-9623