Antimycobacterial activity of the secondary metabolite fraction derived from endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum

Jepri Agung Priyanto

Makale detayları

Antimycobacterial activity of the secondary metabolite fraction derived from endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum

Yazarlar:

Jepri Agung Priyanto

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 5, pp.2055-2063

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001342429

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The discovery and development of new antituberculosis medications effective against multidrug-resistant Mycobacterium strains are necessary to reduce the mortality in tuberculosis TB cases. To accelerate the discovery of anti- TB drugs, Mycobacterium smegmatis was commonly used as a surrogate bacterium in the preliminary screening of antituberculosis compounds. This study aimed to fractionate the secondary metabolites of the endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum, to evaluate its antimycobacterial activity against M. smegmatis, and to identify the secondary metabolite profile of its active fraction. Fifty-six fractions were successfully obtained through thin-layer chromatography TLC and column chromatography techniques. Among all fractions, ten fractions showed remarkable antimycobacterial activity against M. smegmatis with minimum inhibitory concentration MIC values ranging from 851,325 μgml. Fraction F53 exhibited the strongest antimycobacterial activity with the lowest MIC of 85 μgml. This fraction also inhibited the biofilm formation of M. smegmatis at 1 MIC and ½ MIC by 62,3, and 52,1, respectively. Observation using a scanning electron microscope SEM showed that the biofilm matrix of M. smegmatis treated with fraction F53 was thinner, less compact, and separated into small pieces compared to the negative control. Furthermore, liquid chromatography-tandem mass spectrometry LC-MSMS analysis revealed that this corresponding fraction contained five proposed compounds, including 2-p-anisyl-5-methyl-1-hexene, nigakilactone H, tetratriacontanamine, and two other unidentified compounds.

 The discovery and development of new antituberculosis medications effective against multidrug-resistant Mycobacterium strains are necessary to reduce the mortality in tuberculosis TB cases. To accelerate the discovery of anti- TB drugs, Mycobacterium smegmatis was commonly used as a surrogate bacterium in the preliminary screening of antituberculosis compounds. This study aimed to fractionate the secondary metabolites of the endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum, to evaluate its antimycobacterial activity against M. smegmatis, and to identify the secondary metabolite profile of its active fraction. Fifty-six fractions were successfully obtained through thin-layer chromatography TLC and column chromatography techniques. Among all fractions, ten fractions showed remarkable antimycobacterial activity against M. smegmatis with minimum inhibitory concentration MIC values ranging from 851,325 μgml. Fraction F53 exhibited the strongest antimycobacterial activity with the lowest MIC of 85 μgml. This fraction also inhibited the biofilm formation of M. smegmatis at 1 MIC and ½ MIC by 62,3, and 52,1, respectively. Observation using a scanning electron microscope SEM showed that the biofilm matrix of M. smegmatis treated with fraction F53 was thinner, less compact, and separated into small pieces compared to the negative control. Furthermore, liquid chromatography-tandem mass spectrometry LC-MSMS analysis revealed that this corresponding fraction contained five proposed compounds, including 2-p-anisyl-5-methyl-1-hexene, nigakilactone H, tetratriacontanamine, and two other unidentified compounds.

 The discovery and development of new antituberculosis medications effective against multidrug-resistant Mycobacterium strains are necessary to reduce the mortality in tuberculosis TB cases. To accelerate the discovery of anti- TB drugs, Mycobacterium smegmatis was commonly used as a surrogate bacterium in the preliminary screening of antituberculosis compounds. This study aimed to fractionate the secondary metabolites of the endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum, to evaluate its antimycobacterial activity against M. smegmatis, and to identify the secondary metabolite profile of its active fraction. Fifty-six fractions were successfully obtained through thin-layer chromatography TLC and column chromatography techniques. Among all fractions, ten fractions showed remarkable antimycobacterial activity against M. smegmatis with minimum inhibitory concentration MIC values ranging from 851,325 μgml. Fraction F53 exhibited the strongest antimycobacterial activity with the lowest MIC of 85 μgml. This fraction also inhibited the biofilm formation of M. smegmatis at 1 MIC and ½ MIC by 62,3, and 52,1, respectively. Observation using a scanning electron microscope SEM showed that the biofilm matrix of M. smegmatis treated with fraction F53 was thinner, less compact, and separated into small pieces compared to the negative control. Furthermore, liquid chromatography-tandem mass spectrometry LC-MSMS analysis revealed that this corresponding fraction contained five proposed compounds, including 2-p-anisyl-5-methyl-1-hexene, nigakilactone H, tetratriacontanamine, and two other unidentified compounds.

 The discovery and development of new antituberculosis medications effective against multidrug-resistant Mycobacterium strains are necessary to reduce the mortality in tuberculosis TB cases. To accelerate the discovery of anti- TB drugs, Mycobacterium smegmatis was commonly used as a surrogate bacterium in the preliminary screening of antituberculosis compounds. This study aimed to fractionate the secondary metabolites of the endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum, to evaluate its antimycobacterial activity against M. smegmatis, and to identify the secondary metabolite profile of its active fraction. Fifty-six fractions were successfully obtained through thin-layer chromatography TLC and column chromatography techniques. Among all fractions, ten fractions showed remarkable antimycobacterial activity against M. smegmatis with minimum inhibitory concentration MIC values ranging from 851,325 μgml. Fraction F53 exhibited the strongest antimycobacterial activity with the lowest MIC of 85 μgml. This fraction also inhibited the biofilm formation of M. smegmatis at 1 MIC and ½ MIC by 62,3, and 52,1, respectively. Observation using a scanning electron microscope SEM showed that the biofilm matrix of M. smegmatis treated with fraction F53 was thinner, less compact, and separated into small pieces compared to the negative control. Furthermore, liquid chromatography-tandem mass spectrometry LC-MSMS analysis revealed that this corresponding fraction contained five proposed compounds, including 2-p-anisyl-5-methyl-1-hexene, nigakilactone H, tetratriacontanamine, and two other unidentified compounds.

Makale detayları

Antimycobacterial activity of the secondary metabolite fraction derived from endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum

Yazarlar:

Jepri Agung Priyanto

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 5, pp.2055-2063

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001342429

pISSN:

2630-6344

Antimycobacterial

Archidendron pauciflorum

fractionation

MIC

Mycobacterium smegmatis

Sayı

Makale detayları

Antimycobacterial activity of the secondary metabolite fraction derived from endophytic bacterium Bacillus velezensis strain DJ4 isolated from Archidendron pauciflorum

Yazarlar: Jepri Agung Priyanto

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.29 Issue 5, pp.2055-2063

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001342429

pISSN: 2630-6344

Antimycobacterial

Archidendron pauciflorum

fractionation

MIC

Mycobacterium smegmatis

Sayı

Yazarlar:

Jepri Agung Priyanto

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 5, pp.2055-2063

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001342429

pISSN:

2630-6344