Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases

Ayşe ALTUN; Yasemin ERSOY CANILLIOĞLU; Evrim UNSAL; Seda KARABULUT; Canan HURDAG

Makale detayları

Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases

Yazarlar:

Ayşe ALTUN | Yasemin ERSOY CANILLIOĞLU | Evrim UNSAL | Seda KARABULUT | Canan HURDAG

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Experimental and Clinical Medicine 2021 Vol.38 Issue 3, pp.272-276

Belge Tipi:

Makale

Yayın Tarihi:

4/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000507477

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483

Özet:

 

Spermatogenesis is a process that involves cellular and biochemical processes. The purpose of this study is to understand the role of tight junctions in spermatogenesis by evaluating Zonula occludens-1 (ZO-1) protein levels of cells from the testicular sperm extraction (TESE) biopsies in patients with azoospermia 
The study included 40 infertile men with azoospermia. They were divided into two groups, as spermatozoa positive (n=20) and spermatozoa negative (n=20). Testis tissues were examined morphologically, immunohistochemically and ultrastructurally by using transmission electron microscopy (TEM). The structure of seminiferous tubules were deteriorated, degenerated and hyalinized in spermatozoa negative group.  ZO-1 expression level was normal in blood-testis barrier of spermatozoa positive group in comparing to lateral surfaces of sertoli cells spermatozoa negative group. The ultrastructural results were also supported with light microscopy findings. These findings suggest that decrease of  the ZO-1 protein expression level in Sertoli cells might be one of the factors involved in the impairment of spermatogenesis in spermatozoa negative group. Therefore, we suggest that tight junctions may have crucial roles in spermatogenesis.

Makale detayları

Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases

Yazarlar: Ayşe ALTUN | Yasemin ERSOY CANILLIOĞLU | Evrim UNSAL | Seda KARABULUT | Canan HURDAG

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Journal of Experimental and Clinical Medicine 2021 Vol.38 Issue 3, pp.272-276

Belge Tipi: Makale

Yayın Tarihi: 4/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000507477

Sıklık: Üç Aylık

eISSN: 1309-5129

pISSN: 1309-4483

Sayı

Yazarlar:

Ayşe ALTUN | Yasemin ERSOY CANILLIOĞLU | Evrim UNSAL | Seda KARABULUT | Canan HURDAG

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Experimental and Clinical Medicine 2021 Vol.38 Issue 3, pp.272-276

Belge Tipi:

Makale

Yayın Tarihi:

4/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000507477

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483