Investigation of Metallo Beta Lactamases and Oxacilinases in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Inpatients

M. Duygu AKSOY; Şaban ÇAVUŞLU; H. Murat TUĞRUL

Makale detayları

Investigation of Metallo Beta Lactamases and Oxacilinases in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Inpatients

Yazarlar:

M. Duygu AKSOY | Şaban ÇAVUŞLU | H. Murat TUĞRUL

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2015 Vol.32 Issue 1, pp.79-83

Belge Tipi:

Makale

Yayın Tarihi:

12/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000759940

Sıklık:

İki Aylık

pISSN:

2146-3123

Özet:

 

Background: Resistance to beta-lactam antibiotics is
 widespread among Acinetobacter strains. Plasmid-mediated
 metallo beta lactamases (MBL) are responsible
 for carbapenem resistance, as are oxacillinases (OXA).
 In recent years, MBL producing carbapenem-resistant
 strains have been reported in the world and in Turkey
 in increasing rates. In our country, besides the OXA 51-
 like enzyme which is inherent in A. baumannii strains,
 OXA 58-like and OXA 23-like carbapenemases producing
 strains have also been widely detected. In addition,
 Verona Imipenemase (VIM) and (IMP)-type MBL
 have been reported in some centers.
 Aims: The aim of our study was to investigate the presence
 of carbapenemases in Acinetobacter strains isolated
 from hospitalized patients in Edirne.
 Study Design: Cross-sectional study.
 Methods: A total of 52 imipenem-resistant A. baumannii
 strains isolated between January and March 2013
 were investigated. The presence of MBL was described
 phenotypically by the combined disk diffusion test
 (CDDT), double disk synergy test (DDST), MBL Etest
 (only performed in 28 strains) and modified Hodge
 test. blaIMP, blaVIM, blaGIM, blaSIM, blaSPM genes and
 blaOXA-23, blaOXA-51, blaOXA-40, blaOXA-58 genes were investigated
 by multiplex polymerase chain reaction (PCR).
 The blaNDM-1 gene was determined by PCR.
 Results: By modified Hodge test, 50 strains (96%)
 were found to be MBL positive. Positivity of MBL was
 21% by both CDDT (0.1 M EDTA) and DDST. Twenty-four
 of 28 strains (85.7%) were positive by MBL
 E-test. OXA 23-like and OXA 51-like carbapenemases
 were detected in all strains, but OXA 58-like and OXA
 40-like carbapenemases-producing A. baumannii were
 not detected. Also, MBL genes were not detected by
 genotypic methods.
 Conclusion: Only OXA 23-like carbapenemase was
 responsible for carbapenem resistance in carbapenemresistant
 Acinetobacter strains in Edirne. The MBLproducing
 Acinetobacter strain is not yet a problem in
 our hospital. MBL resistance was found by phenotyping
 tests, which must be confirmed by genotypic methods;
 multiplex PCR tests can be easily used for screening
 MBL.

Makale detayları

Investigation of Metallo Beta Lactamases and Oxacilinases in Carbapenem Resistant Acinetobacter baumannii Strains Isolated from Inpatients

Yazarlar: M. Duygu AKSOY | Şaban ÇAVUŞLU | H. Murat TUĞRUL

Yayıncı: Trakya University

Dergi: Balkan Medical Journal 2015 Vol.32 Issue 1, pp.79-83

Belge Tipi: Makale

Yayın Tarihi: 12/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000759940

Sıklık: İki Aylık

pISSN: 2146-3123

Sayı

Yazarlar:

M. Duygu AKSOY | Şaban ÇAVUŞLU | H. Murat TUĞRUL

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2015 Vol.32 Issue 1, pp.79-83

Belge Tipi:

Makale

Yayın Tarihi:

12/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000759940

Sıklık:

İki Aylık

pISSN:

2146-3123