In Vitro Cytotoxicity and Cell Migration Activities of Lipid Extracted from Chlorella Sorokiniana Shihira & R.W.Krauss Exposed to Nitrogen Stress

Betül Yılmaz Öztürk; Bükay Yenice Gürsu; Halima Al-thawr; İlknur Dağ

Makale detayları

In Vitro Cytotoxicity and Cell Migration Activities of Lipid Extracted from Chlorella Sorokiniana Shihira & R.W.Krauss Exposed to Nitrogen Stress

Yazarlar:

Betül Yılmaz Öztürk | Bükay Yenice Gürsu | Halima Al-thawr | İlknur Dağ

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Mikrobiyoloji (Diğer)

Dergi:

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 2, pp.304-311

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356817

pISSN:

1308-6529

Anahtar kelimeler:

Özet:

 Microalgae are used as raw materials in many areas thanks to their valuable biochemical contents. Different environmental conditions significantly affect the biochemical composition of microalgae, and in case of stress, changes are observed in events such as photosynthetic activity in cells, biomass productivity, or pigment formation. Nitrogen is one of the most important nutrient sources for microalgae growth, and in case of nitrogen starvation, the lipid accumulation of microalgae such as Chlorella increases significantly. Although there have been many different studies on the potential use of biochemical components of microalgae, information about the effects and mechanisms of algal oils on wound healing is very limited. Wounds are an important public health problem and carry the risk of infection. New and effective natural alternatives are needed due to reasons such as the long time required for the repair of damaged skin, side effects of existing drugs, or causing allergies or resistance. In this study, the oil produced by C. sorokiniana microalgae in the case of nitrogen starvation and the oils produced in the environment of the control group without nitrogen deprivation were extracted. The in vitro cytotoxic effects of both oils at eight different concentrations 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90 µgmL on the viability of the L929 fibroblast cell line were investigated. No toxic effects were found at any dose studied, and the most effective and lowest dose that increased vitality was determined to be 15.62 µgml. In vitro cell stratching test results revealed that the wound gap closed gradually in the control group, and the oil extract extracted in the case of nitrogen starvation significantly accelerated wound closure over time. It is important to elucidate the therapeutic potential of these oils with further studies and to explain the changes in the oil composition of microalgae in case of stress with advanced analyses.

 Microalgae are used as raw materials in many areas thanks to their valuable biochemical contents. Different environmental conditions significantly affect the biochemical composition of microalgae, and in case of stress, changes are observed in events such as photosynthetic activity in cells, biomass productivity, or pigment formation. Nitrogen is one of the most important nutrient sources for microalgae growth, and in case of nitrogen starvation, the lipid accumulation of microalgae such as Chlorella increases significantly. Although there have been many different studies on the potential use of biochemical components of microalgae, information about the effects and mechanisms of algal oils on wound healing is very limited. Wounds are an important public health problem and carry the risk of infection. New and effective natural alternatives are needed due to reasons such as the long time required for the repair of damaged skin, side effects of existing drugs, or causing allergies or resistance. In this study, the oil produced by C. sorokiniana microalgae in the case of nitrogen starvation and the oils produced in the environment of the control group without nitrogen deprivation were extracted. The in vitro cytotoxic effects of both oils at eight different concentrations 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90 µgmL on the viability of the L929 fibroblast cell line were investigated. No toxic effects were found at any dose studied, and the most effective and lowest dose that increased vitality was determined to be 15.62 µgml. In vitro cell stratching test results revealed that the wound gap closed gradually in the control group, and the oil extract extracted in the case of nitrogen starvation significantly accelerated wound closure over time. It is important to elucidate the therapeutic potential of these oils with further studies and to explain the changes in the oil composition of microalgae in case of stress with advanced analyses.

 Microalgae are used as raw materials in many areas thanks to their valuable biochemical contents. Different environmental conditions significantly affect the biochemical composition of microalgae, and in case of stress, changes are observed in events such as photosynthetic activity in cells, biomass productivity, or pigment formation. Nitrogen is one of the most important nutrient sources for microalgae growth, and in case of nitrogen starvation, the lipid accumulation of microalgae such as Chlorella increases significantly. Although there have been many different studies on the potential use of biochemical components of microalgae, information about the effects and mechanisms of algal oils on wound healing is very limited. Wounds are an important public health problem and carry the risk of infection. New and effective natural alternatives are needed due to reasons such as the long time required for the repair of damaged skin, side effects of existing drugs, or causing allergies or resistance. In this study, the oil produced by C. sorokiniana microalgae in the case of nitrogen starvation and the oils produced in the environment of the control group without nitrogen deprivation were extracted. The in vitro cytotoxic effects of both oils at eight different concentrations 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90 µgmL on the viability of the L929 fibroblast cell line were investigated. No toxic effects were found at any dose studied, and the most effective and lowest dose that increased vitality was determined to be 15.62 µgml. In vitro cell stratching test results revealed that the wound gap closed gradually in the control group, and the oil extract extracted in the case of nitrogen starvation significantly accelerated wound closure over time. It is important to elucidate the therapeutic potential of these oils with further studies and to explain the changes in the oil composition of microalgae in case of stress with advanced analyses.

 Özet: Mikroalgler sahip oldukları değerli biyokimyasal içerikleri sayesinde birçok alanda hammadde olarak kullanılmaktadır. Farklı çevre koşulları mikroalglerin biyokimyasal kompozisyonunu önemli ölçüde etkilemektedir ve stres durumunda hücrelerdeki fotosentetik aktivite, biyokütle verimliliği, ya da pigment oluşumu gibi olaylarda değişiklikler gözlenir. Nitrojen, mikroalg gelişimi için en önemli besin kaynaklarından biridir ve nitrojen açlığı durumunda Chlorella gibi mikroalglerin lipid birikimini önemli ölçüde artar. Şimdiye kadar mikroalglerin sahip olduğu biyokimyasal bileşenlerin kullanım potansiyeli üzerine çok farklı çalışmalar bulunmakla beraber, yara iyileşmesi üzerine algal yağların etki ve mekanizmaları hakkındaki bilgiler çok sınırlıdır. Yara önemli bir halk sağlığı sorunu olup, enfeksiyon riski taşımaktadır. Hasarlı cildin onarımı için gereken sürenin uzun olması, mevcut ilaçların yan etkileri, alerji ya da dirence yol açması gibi sebeplerle yeni ve etkili doğal alternatiflere ihtiyaç bulunmaktadır. Bu çalışmada Chlorella sorokiniana mikroalginin nitrojen açlığı durumunda ürettiği yağ ile nitrojen yoksunluğu olmayan kontrol grubuna ait ortamda ürettikleri yağlar ekstrakte edilmiştir. Her iki yağın sekiz farklı konsantrasyonda 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90 µgmL L929 fibroblast hücre hattı canlılığı üzerine in vitro sitotoksik etkileri araştırılmıştır. Çalışılan hiçbir dozda toksik etki bulunmamış, canlılığı artıran en etkili ve en düşük doz 15.62 µgml olarak tespit edilmiştir. İn vitro cell stratching testi sonuçları kontrol grubunda yara boşluğunun kademeli olarak kapandığını, nitrojen açlığı durumunda ekstrakte edilen yağ ekstraktının ise zamana bağlı olarak yara kapanmasını önemli ölçüde hızlandırdığını ortaya koymuştur. Bu yağların terapötik potansiyellerinin daha ileri çalışmalarla aydınlatılması ve stres durumunda mikroalglerin yağ kompozisyonlarındaki değişimlerin ileri analizlerle açıklanması önem taşımaktadır.

Makale detayları

In Vitro Cytotoxicity and Cell Migration Activities of Lipid Extracted from Chlorella Sorokiniana Shihira & R.W.Krauss Exposed to Nitrogen Stress

Yazarlar:

Betül Yılmaz Öztürk | Bükay Yenice Gürsu | Halima Al-thawr | İlknur Dağ

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Mikrobiyoloji (Diğer)

Dergi:

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 2, pp.304-311

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356817

pISSN:

1308-6529

Chlorella sorokiniana

nitrogen starvation

lipid accumulation

wound healingChlorella sorokiniana

nitrojen açlığı

lipid birikimi

yara iyileşmesi

Sayı

Makale detayları

Yazarlar: Betül Yılmaz Öztürk | Bükay Yenice Gürsu | Halima Al-thawr | İlknur Dağ

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Hücre Gelişimi | Proliferasyon ve Ölümü | Mikrobiyoloji (Diğer)

Dergi: Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 2, pp.304-311

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356817

pISSN: 1308-6529

Sayı

Yazarlar:

Betül Yılmaz Öztürk | Bükay Yenice Gürsu | Halima Al-thawr | İlknur Dağ

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Mikrobiyoloji (Diğer)

Dergi:

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.29 Issue 2, pp.304-311

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356817

pISSN:

1308-6529