LOW-DOSE LUTEOLIN FAILS TO MITIGATE DOXORUBICIN-INDUCED CARDIOVASCULAR AND PULMONARY INJURY IN RATS: HISTOPATHOLOGICAL STUDY

Kübra Şevgin; Yağmur Çelik; Nur Elagül Tombul; Ayşe Köse Vuruşkan

Makale detayları

LOW-DOSE LUTEOLIN FAILS TO MITIGATE DOXORUBICIN-INDUCED CARDIOVASCULAR AND PULMONARY INJURY IN RATS: HISTOPATHOLOGICAL STUDY

Yazarlar:

Kübra Şevgin | Yağmur Çelik | Nur Elagül Tombul | Ayşe Köse Vuruşkan

Yayıncı:

Inonu University

Ana Başlıklar:

Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 3, pp.778-790

Belge Tipi:

Makale

Yayın Tarihi:

10/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359995

pISSN:

2147-7892

Anahtar kelimeler:

Özet:

 This study aimed to evaluate the effect of luteolin LUT on lung, heart, and aorta histology in rats subjected to doxorubicin DOX-induced injury. Thirty-four 8-week-old male rats were divided to four groups: control saline, LUT 20 μgkgday, DOX 5 mgkg on days 1, 7, 14, 21, 28, and DOXLUT combined treatment. On day 29, rats were sacrificed for histological assessment of cardiovascular and pulmonary damage. Heart, lung, and aorta tissues were stained with Hematoxylin and Eosin HE, Toluidine Blue TB, Safranin and Hemalum SH, or Picrosirius Red PSR. Semi-quantitative analyses of collagen and elastic fibers was performed using ImageJ. Group comparisons were made using one-way ANOVA with Bonferroni correction p  0.0083. DOX induced pronounced myocardial injury evidenced by loss of cross-striations, cytoplasmic vacuolization, coagulative necrosis, and an increase in collagen deposition with reduction in aortic elastic fibers and increase in aortic-collagen, as well as alveolar septal thickening, hemorrhage, and an increase in pulmonary mast cell density and interstitial collagen all p  0.0083. LUT treatment alone produced no histological alterations, and its co-administration at 20 µgkg failed to ameliorate any of the DOX-induced changes. In conclusion, LUT 20 µgkg did not alleviate DOX-induced cardiopulmonary damage, underscoring the need for dose optimization in future studies.

 This study aimed to evaluate the effect of luteolin LUT on lung, heart, and aorta histology in rats subjected to doxorubicin DOX-induced injury. Thirty-four 8-week-old male rats were divided to four groups: control saline, LUT 20 μgkgday, DOX 5 mgkg on days 1, 7, 14, 21, 28, and DOXLUT combined treatment. On day 29, rats were sacrificed for histological assessment of cardiovascular and pulmonary damage. Heart, lung, and aorta tissues were stained with Hematoxylin and Eosin HE, Toluidine Blue TB, Safranin and Hemalum SH, or Picrosirius Red PSR. Semi-quantitative analyses of collagen and elastic fibers was performed using ImageJ. Group comparisons were made using one-way ANOVA with Bonferroni correction p  0.0083. DOX induced pronounced myocardial injury evidenced by loss of cross-striations, cytoplasmic vacuolization, coagulative necrosis, and an increase in collagen deposition with reduction in aortic elastic fibers and increase in aortic-collagen, as well as alveolar septal thickening, hemorrhage, and an increase in pulmonary mast cell density and interstitial collagen all p  0.0083. LUT treatment alone produced no histological alterations, and its co-administration at 20 µgkg failed to ameliorate any of the DOX-induced changes. In conclusion, LUT 20 µgkg did not alleviate DOX-induced cardiopulmonary damage, underscoring the need for dose optimization in future studies.

 This study aimed to evaluate the effect of luteolin LUT on lung, heart, and aorta histology in rats subjected to doxorubicin DOX-induced injury. Thirty-four 8-week-old male rats were divided to four groups: control saline, LUT 20 μgkgday, DOX 5 mgkg on days 1, 7, 14, 21, 28, and DOXLUT combined treatment. On day 29, rats were sacrificed for histological assessment of cardiovascular and pulmonary damage. Heart, lung, and aorta tissues were stained with Hematoxylin and Eosin HE, Toluidine Blue TB, Safranin and Hemalum SH, or Picrosirius Red PSR. Semi-quantitative analyses of collagen and elastic fibers was performed using ImageJ. Group comparisons were made using one-way ANOVA with Bonferroni correction p  0.0083. DOX induced pronounced myocardial injury evidenced by loss of cross-striations, cytoplasmic vacuolization, coagulative necrosis, and an increase in collagen deposition with reduction in aortic elastic fibers and increase in aortic-collagen, as well as alveolar septal thickening, hemorrhage, and an increase in pulmonary mast cell density and interstitial collagen all p  0.0083. LUT treatment alone produced no histological alterations, and its co-administration at 20 µgkg failed to ameliorate any of the DOX-induced changes. In conclusion, LUT 20 µgkg did not alleviate DOX-induced cardiopulmonary damage, underscoring the need for dose optimization in future studies.

 Bu çalışmanın amacı doksorubisin DOX ile indüklenen hasara maruz kalan sıçanlarda luteolinin LUT akciğer, kalp ve aort histolojisi üzerindeki etkisini değerlendirmektir. Otuz dört-adet 8-haftalık erkek sıçan dört gruba ayrılmıştır: kontrol salin, LUT 20 μgkggün, DOX 1, 7, 14, 21, 28. günlerde 5 mgkg ve DOXLUT kombine tedavi. 29. günde sıçanlar kardiyovasküler ve pulmoner hasarın histolojik değerlendirmesi için sakrifiye edilmiştir. Kalp, akciğer ve aort dokuları Hematoksilen  Eozin HE, Toluidin Mavisi TB, Safranin  Hemalum SH veya Picrosirius Kırmızısı PSR ile boyandı. Kolajen ve elastik liflerin yarı kantitatif analizi ImageJ kullanılarak gerçekleştirilmiştir. Grup karşılaştırmaları Bonferroni düzeltmeli tek yönlü ANOVA kullanılarak yapılmıştır p  0.0083. DOX, kas çizgilenmesinin kaybı, sitoplazmik vakuolizasyon, koagülatif nekroz ve aortik elastik liflerde azalma ve aortik kolajende artış ile kolajen birikiminde artışın yanı sıra alveolar septal kalınlaşma, hemoraji ve pulmoner mast hücre yoğunluğunda ve interstisyel kolajende artış ile kanıtlanan belirgin miyokardiyal hasara neden olmuştur tümü p  0.0083. Tek başına LUT tedavisi hiçbir histolojik değişikliğe yol açmamış ve 20 µgkg dozunda birlikte uygulanması DOX ile indüklenen değişikliklerin hiçbirini anlamlı derecede iyileştirmemiştir. Sonuç olarak, LUT 20 µgkg DOX ile indüklenen kardiyopulmoner hasarı hafifletmemiştir ve gelecekteki çalışmalarda doz optimizasyonu ihtiyacının altını çizmektedir.

Makale detayları

LOW-DOSE LUTEOLIN FAILS TO MITIGATE DOXORUBICIN-INDUCED CARDIOVASCULAR AND PULMONARY INJURY IN RATS: HISTOPATHOLOGICAL STUDY

Yazarlar:

Kübra Şevgin | Yağmur Çelik | Nur Elagül Tombul | Ayşe Köse Vuruşkan

Yayıncı:

Inonu University

Ana Başlıklar:

Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 3, pp.778-790

Belge Tipi:

Makale

Yayın Tarihi:

10/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359995

pISSN:

2147-7892

Aorta

Doxorubicin

Heart

Lung

LuteolinAort

Doksorubisin

Kalp

Akciğer

Luteolin

Sayı

Makale detayları

Yazarlar: Kübra Şevgin | Yağmur Çelik | Nur Elagül Tombul | Ayşe Köse Vuruşkan

Yayıncı: Inonu University

Ana Başlıklar: Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi: İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 3, pp.778-790

Belge Tipi: Makale

Yayın Tarihi: 10/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359995

pISSN: 2147-7892

Sayı

Yazarlar:

Kübra Şevgin | Yağmur Çelik | Nur Elagül Tombul | Ayşe Köse Vuruşkan

Yayıncı:

Inonu University

Ana Başlıklar:

Geleneksel | Tamamlayıcı ve Bütünleştirici Tıp (Diğer)

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 3, pp.778-790

Belge Tipi:

Makale

Yayın Tarihi:

10/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359995

pISSN:

2147-7892