Sequential Green Extraction of Caffeine and Catechins from Green Tea

Gönül SERDAR; Ezgi DEMİR; Münevver SÖKMEN

doi:https://doi.org/10.21448/ijsm.629151

Makale detayları

Sequential Green Extraction of Caffeine and Catechins from Green Tea

Yazarlar:

Gönül SERDAR | Ezgi DEMİR | Münevver SÖKMEN

Yayıncı:

İzzet KARA

Ana Başlıklar:

Yaşam bilimleri

Dergi:

International Journal of Secondary Metabolite 2019 Vol.6 Issue 3, pp.283-291

Belge Tipi:

Makale

Yayın Tarihi:

10/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000471567

Sıklık:

Üç Aylık

eISSN:

2148-6905

DOI:

https://doi.org/10.21448/ijsm.629151

Özet:

 


Separation of caffeine and catechins from tea extracts usually requires conventional liquid-liquid extraction employing chloroform.  This work was planned to improve a green extraction technique to distunguish these important chemicals from green tea. Extraction of caffeine and catechins from Turkish green tea firstly employing MAE, and then to separate these compounds from each other using a SFE method were purpose of this study. Microwave assisted extraction was applied to extract tea components from green tea then i) conventional liquid-liquid extraction or ii) supercritical carbon dioxide fluid extraction (SFE) method was charged with the effective separation of caffeine and catechins. Initially, an ethanol: water mixture was used in a close microwave system under the particuler extraction situations of green tea samples (fresh, frozen or dried) picked up in three collection periods (first, second and third collection periods). MAE of tea samples was exerted under a controlled 600 W microwave power for 4 min irradiation time at 80 oC temperature. Then MAE crude aqueous extract was divided in to two portions. The first portion was fractionated first with chloroform to distunguish caffeine then ethyl acetate for catechins. Caffeine and catechins were successfully separated. Second portion was freeze-dried and obtained lyophilized solid was used for SFE. Caffeine (3.68% extract yield)  was successfully separated from catechins with SFE at 250 bar and 60°C for 180 min. Constituents of the extracts were determined (caffeine and four catechins namely EGC, EC, C, EGCG) by HPLC to evaluate the effectiveness of the separation.

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Makale detayları

Sequential Green Extraction of Caffeine and Catechins from Green Tea

Yazarlar: Gönül SERDAR | Ezgi DEMİR | Münevver SÖKMEN

Yayıncı: İzzet KARA

Ana Başlıklar: Yaşam bilimleri

Dergi: International Journal of Secondary Metabolite 2019 Vol.6 Issue 3, pp.283-291

Belge Tipi: Makale

Yayın Tarihi: 10/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000471567

Sıklık: Üç Aylık

eISSN: 2148-6905

DOI: https://doi.org/10.21448/ijsm.629151

Sayı

Yazarlar:

Gönül SERDAR | Ezgi DEMİR | Münevver SÖKMEN

Yayıncı:

İzzet KARA

Ana Başlıklar:

Yaşam bilimleri

Dergi:

International Journal of Secondary Metabolite 2019 Vol.6 Issue 3, pp.283-291

Belge Tipi:

Makale

Yayın Tarihi:

10/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000471567

Sıklık:

Üç Aylık

eISSN:

2148-6905

DOI:

https://doi.org/10.21448/ijsm.629151