COMPARISON OF THE IMMUNOMODULATORY PROPERTIES OF THE CORD BLOOD AND WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS

Ayşe Erol; Figen Abatay Sel; Mediha Süleymanoğlu; Gökhan Demirayak; Dürdane Serap Kuruca; Fatma Savran Oğuz

Makale detayları

COMPARISON OF THE IMMUNOMODULATORY PROPERTIES OF THE CORD BLOOD AND WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS

Yazarlar:

Ayşe Erol | Figen Abatay Sel | Mediha Süleymanoğlu | Gökhan Demirayak | Dürdane Serap Kuruca | Fatma Savran Oğuz

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.136-142

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302266

pISSN:

2651-4060

Anahtar kelimeler:

Özet:

 Objective: Mesenchymal stem cells MSCs are promising as a treatment option for many immune-related diseases due to their immune regulatory properties. Whartons Jelly WJ-MSC and cord blood CB-MSC have recently received more attention than the other MSC sources. In this study, it was aimed to investigate the difference in the mechanisms of the immunological effects of WJ-MSCs and CB-MSCs. Material and Methods: The intracellular cytokine levels of peripheral blood mononuclear cells PBMC and CD4 T cells before and after MSC co-culture Interleukin-4 IL-4, Interferon-γ IFN-γ, and Interleukin-17 IL17 were determined by flow cytometry. At the same time, tumor growth factor TGF-β, IL-4, IL-17, IFN-γ supernatant cytokine levels were measured by ELISA. In the study, incubation times of 24 hours and 72 hours were applied with co-culture MSCPBMC ratios of 15 and 110. Results: Our data showed that WJ-MSCs and CB-MSCs have different morphological features, proliferation capacities, proliferation times and immunomodulating abilities. One of the cytokines of IFN-γ decreased significantly at both 15 and 110 ratios in the cell at 24 hours and increased significantly at 72 hours after CB-MSCPBMC co-culture compared to the level after WJ-MSCPBMC co-culture p0.05. Unlike for IL-17 cytokine, the intracellular level decreased significantly in the CB-MSC group only at 72 hours compared to the WJ-MSC group. For IL-4, the 110 ratio in the CB-MSC group decreased significantly at 24 hours, while the intracellular level was increased in all other groups. After CB-MSCPBMC co-culture, TGF-β supernatant level decreased by 15 in 24 hours and increased in 72 hours. On the contrary, TGF-β supernatant level increased in the CB-MSC group at 24 hours and decreased significantly at 72 hours in 110 ratios p0.05. Conclusion: CB-MSCs and WJ-MSCs show different immunomodulatory properties. Based on these findings, it can be said that the use of WJ-MSCs is more advantageous in terms of cell therapies due to the different isolation, proliferation capacity and immunomodulatory properties of CB-MSCs.

 Objective: Mesenchymal stem cells MSCs are promising as a treatment option for many immune-related diseases due to their immune regulatory properties. Whartons Jelly WJ-MSC and cord blood CB-MSC have recently received more attention than the other MSC sources. In this study, it was aimed to investigate the difference in the mechanisms of the immunological effects of WJ-MSCs and CB-MSCs. Material and Methods: The intracellular cytokine levels of peripheral blood mononuclear cells PBMC and CD4 T cells before and after MSC co-culture Interleukin-4 IL-4, Interferon-γ IFN-γ, and Interleukin-17 IL17 were determined by flow cytometry. At the same time, tumor growth factor TGF-β, IL-4, IL-17, IFN-γ supernatant cytokine levels were measured by ELISA. In the study, incubation times of 24 hours and 72 hours were applied with co-culture MSCPBMC ratios of 15 and 110. Results: Our data showed that WJ-MSCs and CB-MSCs have different morphological features, proliferation capacities, proliferation times and immunomodulating abilities. One of the cytokines of IFN-γ decreased significantly at both 15 and 110 ratios in the cell at 24 hours and increased significantly at 72 hours after CB-MSCPBMC co-culture compared to the level after WJ-MSCPBMC co-culture p0.05. Unlike for IL-17 cytokine, the intracellular level decreased significantly in the CB-MSC group only at 72 hours compared to the WJ-MSC group. For IL-4, the 110 ratio in the CB-MSC group decreased significantly at 24 hours, while the intracellular level was increased in all other groups. After CB-MSCPBMC co-culture, TGF-β supernatant level decreased by 15 in 24 hours and increased in 72 hours. On the contrary, TGF-β supernatant level increased in the CB-MSC group at 24 hours and decreased significantly at 72 hours in 110 ratios p0.05. Conclusion: CB-MSCs and WJ-MSCs show different immunomodulatory properties. Based on these findings, it can be said that the use of WJ-MSCs is more advantageous in terms of cell therapies due to the different isolation, proliferation capacity and immunomodulatory properties of CB-MSCs.

 Objective: Mesenchymal stem cells MSCs are promising as a treatment option for many immune-related diseases due to their immune regulatory properties. Whartons Jelly WJ-MSC and cord blood CB-MSC have recently received more attention than the other MSC sources. In this study, it was aimed to investigate the difference in the mechanisms of the immunological effects of WJ-MSCs and CB-MSCs. Material and Methods: The intracellular cytokine levels of peripheral blood mononuclear cells PBMC and CD4 T cells before and after MSC co-culture Interleukin-4 IL-4, Interferon-γ IFN-γ, and Interleukin-17 IL17 were determined by flow cytometry. At the same time, tumor growth factor TGF-β, IL-4, IL-17, IFN-γ supernatant cytokine levels were measured by ELISA. In the study, incubation times of 24 hours and 72 hours were applied with co-culture MSCPBMC ratios of 15 and 110. Results: Our data showed that WJ-MSCs and CB-MSCs have different morphological features, proliferation capacities, proliferation times and immunomodulating abilities. One of the cytokines of IFN-γ decreased significantly at both 15 and 110 ratios in the cell at 24 hours and increased significantly at 72 hours after CB-MSCPBMC co-culture compared to the level after WJ-MSCPBMC co-culture p0.05. Unlike for IL-17 cytokine, the intracellular level decreased significantly in the CB-MSC group only at 72 hours compared to the WJ-MSC group. For IL-4, the 110 ratio in the CB-MSC group decreased significantly at 24 hours, while the intracellular level was increased in all other groups. After CB-MSCPBMC co-culture, TGF-β supernatant level decreased by 15 in 24 hours and increased in 72 hours. On the contrary, TGF-β supernatant level increased in the CB-MSC group at 24 hours and decreased significantly at 72 hours in 110 ratios p0.05. Conclusion: CB-MSCs and WJ-MSCs show different immunomodulatory properties. Based on these findings, it can be said that the use of WJ-MSCs is more advantageous in terms of cell therapies due to the different isolation, proliferation capacity and immunomodulatory properties of CB-MSCs.

 Amaç: Mezenkimal kök hücreler MKHler, immün düzenleyici özellikleri nedeniyle bir çok immün sistem ilişkili hastalık için tedavi seçeneği olarak umut vaad etmektedir. Wharton Jeli WJ-MKH ve kordon kanı KK-MKH, diğer MKH kaynaklarına göre son dönemlerde daha çok ilgi görmektedir. Çalışmada, WJ-MKHler ve KK-MKHlerin immünolojik etkilerinin mekanizmalarındaki farkı araştırmak amaçlanmıştır. Gereç ve Yöntem: Periferik kan mononükler hücreler PKMH ve MKH ko-kültür öncesi ve sonrası CD4 T hücrelerin hücre içi sitokin seviyeleri Interlökin-4 IL-4, Interferon-γ IFN-γ, and Interlökin-17 IL-17 flow sitometri ile tespit edildi. Aynı zamanda Tümör büyüme faktörü TGF-β, IL-4, IL-17, IFN-γ süpernatant sitokin seviyeleri ELISA ile ölçüldü. Çalışmada, ko-kültür MKHPKMH 15 ve 110 oranlarında, 24 saat ve 72 saat inkübasyon süreleri uygulandı. Bulgular: Verilerimiz WJ-MKHlerin ve KK-MKHlerin farklı morfolojik özellikleri, çoğalma kapasiteleri, çoğalma süreleri ve immünomodülasyon yetenekleri olduğunu gösterdi. IFN-γ, KK-MKHPKMH ko-kültür sonrası WJ-MKHPKMH ko-kültürü sonrasına göre hücre içerisinde hem 15 hem 110 oranlarında 24. saatlerde anlamlı derecede azalırken, 72. saatlerde anlamlı derecede arttı p0,05. IL-17 sitokinin için farklı olarak sadece 72. saatte KK-MKH grubunda WJ-MKH grubuna göre anlamlı derecede hücre içi seviyesi düştü. IL-4 için KK-MKH grubunda 110 oran 24 saatte hücre içi seviyesi anlamlı derecede düşerken diğer tüm gruplarda artış gösterdi. KK-MKHPKMH ko-kültür sonrası TGF-β supernatant seviyesi 15 oranında 24 saatte azalırken 72 saatte artış gösterdi. 110 oranlarında ise tam tersi 24. saatte KK-MKH grubunda artarken 72 saatlerde anlamlı derecede azaldı p0,05. Sonuç: KK-MKHler ve WJ-MKHler, farklı immünmodülatör özellikler göstermektedir. Bu bulgular, KK-MKHlerin izolasyonun, çoğalma kapasitesinin ve immünmodülatör özelliklerinin farklı olması sebebiyle hücre tedavileri açısından WJ-MKHlerin kullanılmasının daha avantajlı olduğu söylenebilir.

Makale detayları

COMPARISON OF THE IMMUNOMODULATORY PROPERTIES OF THE CORD BLOOD AND WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS

Yazarlar:

Ayşe Erol | Figen Abatay Sel | Mediha Süleymanoğlu | Gökhan Demirayak | Dürdane Serap Kuruca | Fatma Savran Oğuz

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.136-142

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302266

pISSN:

2651-4060

Cord blood

Cord tissue

MSCs

ImmunomodulationKordon kanı

Kordon dokusu

mezenkimal kök hücre

PKMH

ko-kültür

immunmodülasyon

Sayı

Makale detayları

Yazarlar: Ayşe Erol | Figen Abatay Sel | Mediha Süleymanoğlu | Gökhan Demirayak | Dürdane Serap Kuruca | Fatma Savran Oğuz

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences

Dergi: Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.136-142

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302266

pISSN: 2651-4060

Sayı

Yazarlar:

Ayşe Erol | Figen Abatay Sel | Mediha Süleymanoğlu | Gökhan Demirayak | Dürdane Serap Kuruca | Fatma Savran Oğuz

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.136-142

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302266

pISSN:

2651-4060