Effects of bufadıenolıdes from Bufo vırıdıs toad venom on blood biochemical compositions and thromboelastographic parameters

N.A.

Makale detayları

Effects of bufadıenolıdes from Bufo vırıdıs toad venom on blood biochemical compositions and thromboelastographic parameters

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.995-1003

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347892

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Bufadienolides, cyclopentanophenantrene derivatives that possess similar structures to cardiac glycosides, have been established to reveal cytotoxicity. Their higher concentrations as anticancer means are expected to affect the normal function of the heart, liver, and kidney. In this work, we established the mass ratio of bufadienolides isolated from the venom of Bufo viridis. Arenobufagin, gamabufotalin, telocinobufagin, and marinobufagin were found as the major compounds in the sum. Their effects on biological parameters, used as indicators of the normal functioning of organs, were determined in rabbits. The blood samples, taken on the 10th and 30th days following the drug administration, showed these paramaters reaching the control levels for 30 days in all studied doses: 0.15, 0.45, and 0.6 mgkg. The 0.15 and 0.6 mgkg doses were established not to cause significant changes in the activities of alanine aminotransferase and aspartate aminotransferase, and the quantities of cholesterol and fasting blood sugar in blood samples. Significant changes were found in quantities of glucose a reduction and urea an increase on the 10th day of the administration. But their levels reached the control level for the next 20 days. We observed significant differences in thromboelastographic parameters in mice on the 10th day after the drug administration in a 0.15 mgkg dose. In mice, LD50 levels of intravenous, subcutaneous, and oral administrations made 14.5, 110, and 215 mgkg, respectively. These results enable the suggestion of optimum concentration that does not cause severe damage to the functions of organs.

 Bufadienolides, cyclopentanophenantrene derivatives that possess similar structures to cardiac glycosides, have been established to reveal cytotoxicity. Their higher concentrations as anticancer means are expected to affect the normal function of the heart, liver, and kidney. In this work, we established the mass ratio of bufadienolides isolated from the venom of Bufo viridis. Arenobufagin, gamabufotalin, telocinobufagin, and marinobufagin were found as the major compounds in the sum. Their effects on biological parameters, used as indicators of the normal functioning of organs, were determined in rabbits. The blood samples, taken on the 10th and 30th days following the drug administration, showed these paramaters reaching the control levels for 30 days in all studied doses: 0.15, 0.45, and 0.6 mgkg. The 0.15 and 0.6 mgkg doses were established not to cause significant changes in the activities of alanine aminotransferase and aspartate aminotransferase, and the quantities of cholesterol and fasting blood sugar in blood samples. Significant changes were found in quantities of glucose a reduction and urea an increase on the 10th day of the administration. But their levels reached the control level for the next 20 days. We observed significant differences in thromboelastographic parameters in mice on the 10th day after the drug administration in a 0.15 mgkg dose. In mice, LD50 levels of intravenous, subcutaneous, and oral administrations made 14.5, 110, and 215 mgkg, respectively. These results enable the suggestion of optimum concentration that does not cause severe damage to the functions of organs.

 Bufadienolides, cyclopentanophenantrene derivatives that possess similar structures to cardiac glycosides, have been established to reveal cytotoxicity. Their higher concentrations as anticancer means are expected to affect the normal function of the heart, liver, and kidney. In this work, we established the mass ratio of bufadienolides isolated from the venom of Bufo viridis. Arenobufagin, gamabufotalin, telocinobufagin, and marinobufagin were found as the major compounds in the sum. Their effects on biological parameters, used as indicators of the normal functioning of organs, were determined in rabbits. The blood samples, taken on the 10th and 30th days following the drug administration, showed these paramaters reaching the control levels for 30 days in all studied doses: 0.15, 0.45, and 0.6 mgkg. The 0.15 and 0.6 mgkg doses were established not to cause significant changes in the activities of alanine aminotransferase and aspartate aminotransferase, and the quantities of cholesterol and fasting blood sugar in blood samples. Significant changes were found in quantities of glucose a reduction and urea an increase on the 10th day of the administration. But their levels reached the control level for the next 20 days. We observed significant differences in thromboelastographic parameters in mice on the 10th day after the drug administration in a 0.15 mgkg dose. In mice, LD50 levels of intravenous, subcutaneous, and oral administrations made 14.5, 110, and 215 mgkg, respectively. These results enable the suggestion of optimum concentration that does not cause severe damage to the functions of organs.

 Bufadienolides, cyclopentanophenantrene derivatives that possess similar structures to cardiac glycosides, have been established to reveal cytotoxicity. Their higher concentrations as anticancer means are expected to affect the normal function of the heart, liver, and kidney. In this work, we established the mass ratio of bufadienolides isolated from the venom of Bufo viridis. Arenobufagin, gamabufotalin, telocinobufagin, and marinobufagin were found as the major compounds in the sum. Their effects on biological parameters, used as indicators of the normal functioning of organs, were determined in rabbits. The blood samples, taken on the 10th and 30th days following the drug administration, showed these paramaters reaching the control levels for 30 days in all studied doses: 0.15, 0.45, and 0.6 mgkg. The 0.15 and 0.6 mgkg doses were established not to cause significant changes in the activities of alanine aminotransferase and aspartate aminotransferase, and the quantities of cholesterol and fasting blood sugar in blood samples. Significant changes were found in quantities of glucose a reduction and urea an increase on the 10th day of the administration. But their levels reached the control level for the next 20 days. We observed significant differences in thromboelastographic parameters in mice on the 10th day after the drug administration in a 0.15 mgkg dose. In mice, LD50 levels of intravenous, subcutaneous, and oral administrations made 14.5, 110, and 215 mgkg, respectively. These results enable the suggestion of optimum concentration that does not cause severe damage to the functions of organs.

Makale detayları

Effects of bufadıenolıdes from Bufo vırıdıs toad venom on blood biochemical compositions and thromboelastographic parameters

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.995-1003

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347892

pISSN:

2630-6344

Toad venom

bufadienolides

acute toxicity

chronic toxicity

glucose

urea.

Sayı

Makale detayları

Effects of bufadıenolıdes from Bufo vırıdıs toad venom on blood biochemical compositions and thromboelastographic parameters

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.27 Issue 3, pp.995-1003

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347892

pISSN: 2630-6344

Toad venom

bufadienolides

acute toxicity

chronic toxicity

glucose

urea.

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 3, pp.995-1003

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347892

pISSN:

2630-6344