The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines

Yasin Çelikok; Leyla Turker Sener; Burcu Bütün; Işıl Albeniz

Makale detayları

The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines

Yazarlar:

Yasin Çelikok | Leyla Turker Sener | Burcu Bütün | Işıl Albeniz

Yayıncı:

Istanbul University

Ana Başlıklar:

Health Care Administration

Dergi:

İstanbul Journal of Pharmacy Vol.53 Issue 2, pp.186-192

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300306

pISSN:

2587-2087

Anahtar kelimeler:

Özet:

 Background and Aims: Plant extracts are an important source of cytotoxic compounds and have consistently been an interesting field of research. The aim of this study is to investigate the cytotoxic and apoptotic effects of Thymus vulgaris T. vulgaris extracts on human breast cancer cell lines. Methods: This study was carried out using human breast cancer cell lines MCF-7 and MDA-MB-231 as experimental groups and the healthy human fibroblast cell line PCS-201-012 as the control group. Petroleum ether and ethanol extracts were obtained from T. vulgaris. The extracts were applied to MCF-7 and MDA-MB-231 human breast cancer cell lines and human breast cancer stem cells. Cytotoxicity studies were performed using the RTCA iCELLigence system Agilent Technologies, and apoptosis studies were performed using terminal deoxynucleotidyl transferase TdT dUTP nick-end labeling TUNEL and 4,6-diamidino-2 phenylindole DAPI methods. Results: The T. vulgaris extracts were found to have concentration-dependent cytotoxic effects on human breast cancer cells. The growth of breast cancer stem cells was also determined to be inhibited when an effective concentration 45 μg mL of the extracts was applied. Lastly, specific morphological changes related to apoptosis were detected in the cells that had been treated with the effective concentration. Conclusion: The T. vulgaris extracts were found to inhibit the proliferation of human breast cancer cells and human breast cancer stem cells selectively and concentration-dependently via an apoptosis-dependent pathway. The results suggest that the extracts may make promising sources for developing drugs for breast cancer therapy.

 Background and Aims: Plant extracts are an important source of cytotoxic compounds and have consistently been an interesting field of research. The aim of this study is to investigate the cytotoxic and apoptotic effects of Thymus vulgaris T. vulgaris extracts on human breast cancer cell lines. Methods: This study was carried out using human breast cancer cell lines MCF-7 and MDA-MB-231 as experimental groups and the healthy human fibroblast cell line PCS-201-012 as the control group. Petroleum ether and ethanol extracts were obtained from T. vulgaris. The extracts were applied to MCF-7 and MDA-MB-231 human breast cancer cell lines and human breast cancer stem cells. Cytotoxicity studies were performed using the RTCA iCELLigence system Agilent Technologies, and apoptosis studies were performed using terminal deoxynucleotidyl transferase TdT dUTP nick-end labeling TUNEL and 4,6-diamidino-2 phenylindole DAPI methods. Results: The T. vulgaris extracts were found to have concentration-dependent cytotoxic effects on human breast cancer cells. The growth of breast cancer stem cells was also determined to be inhibited when an effective concentration 45 μg mL of the extracts was applied. Lastly, specific morphological changes related to apoptosis were detected in the cells that had been treated with the effective concentration. Conclusion: The T. vulgaris extracts were found to inhibit the proliferation of human breast cancer cells and human breast cancer stem cells selectively and concentration-dependently via an apoptosis-dependent pathway. The results suggest that the extracts may make promising sources for developing drugs for breast cancer therapy.

 Background and Aims: Plant extracts are an important source of cytotoxic compounds and have consistently been an interesting field of research. The aim of this study is to investigate the cytotoxic and apoptotic effects of Thymus vulgaris T. vulgaris extracts on human breast cancer cell lines. Methods: This study was carried out using human breast cancer cell lines MCF-7 and MDA-MB-231 as experimental groups and the healthy human fibroblast cell line PCS-201-012 as the control group. Petroleum ether and ethanol extracts were obtained from T. vulgaris. The extracts were applied to MCF-7 and MDA-MB-231 human breast cancer cell lines and human breast cancer stem cells. Cytotoxicity studies were performed using the RTCA iCELLigence system Agilent Technologies, and apoptosis studies were performed using terminal deoxynucleotidyl transferase TdT dUTP nick-end labeling TUNEL and 4,6-diamidino-2 phenylindole DAPI methods. Results: The T. vulgaris extracts were found to have concentration-dependent cytotoxic effects on human breast cancer cells. The growth of breast cancer stem cells was also determined to be inhibited when an effective concentration 45 μg mL of the extracts was applied. Lastly, specific morphological changes related to apoptosis were detected in the cells that had been treated with the effective concentration. Conclusion: The T. vulgaris extracts were found to inhibit the proliferation of human breast cancer cells and human breast cancer stem cells selectively and concentration-dependently via an apoptosis-dependent pathway. The results suggest that the extracts may make promising sources for developing drugs for breast cancer therapy.

 Background and Aims: Plant extracts are an important source of cytotoxic compounds and have consistently been an interesting field of research. The aim of this study is to investigate the cytotoxic and apoptotic effects of Thymus vulgaris T. vulgaris extracts on human breast cancer cell lines. Methods: This study was carried out using human breast cancer cell lines MCF-7 and MDA-MB-231 as experimental groups and the healthy human fibroblast cell line PCS-201-012 as the control group. Petroleum ether and ethanol extracts were obtained from T. vulgaris. The extracts were applied to MCF-7 and MDA-MB-231 human breast cancer cell lines and human breast cancer stem cells. Cytotoxicity studies were performed using the RTCA iCELLigence system Agilent Technologies, and apoptosis studies were performed using terminal deoxynucleotidyl transferase TdT dUTP nick-end labeling TUNEL and 4,6-diamidino-2 phenylindole DAPI methods. Results: The T. vulgaris extracts were found to have concentration-dependent cytotoxic effects on human breast cancer cells. The growth of breast cancer stem cells was also determined to be inhibited when an effective concentration 45 μg mL of the extracts was applied. Lastly, specific morphological changes related to apoptosis were detected in the cells that had been treated with the effective concentration. Conclusion: The T. vulgaris extracts were found to inhibit the proliferation of human breast cancer cells and human breast cancer stem cells selectively and concentration-dependently via an apoptosis-dependent pathway. The results suggest that the extracts may make promising sources for developing drugs for breast cancer therapy.

Makale detayları

The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines

Yazarlar: Yasin Çelikok | Leyla Turker Sener | Burcu Bütün | Işıl Albeniz

Yayıncı: Istanbul University

Ana Başlıklar: Health Care Administration

Dergi: İstanbul Journal of Pharmacy Vol.53 Issue 2, pp.186-192

Belge Tipi: Makale

Yayın Tarihi: 8/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001300306

pISSN: 2587-2087

DAPI

iCELLigence

MCF-7

MDA-MB-231

Thymus vulgaris

TUNEL

Sayı

Yazarlar:

Yasin Çelikok | Leyla Turker Sener | Burcu Bütün | Işıl Albeniz

Yayıncı:

Istanbul University

Ana Başlıklar:

Health Care Administration

Dergi:

İstanbul Journal of Pharmacy Vol.53 Issue 2, pp.186-192

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300306

pISSN:

2587-2087