Evaluation of anti-proliferative and cytotoxic effects of chlorogenic acid on breast cancer cell lines by real-time, label-free and high-throughput screening

Onur Bender; Arzu Atalay

Makale detayları

Evaluation of anti-proliferative and cytotoxic effects of chlorogenic acid on breast cancer cell lines by real-time, label-free and high-throughput screening

Yazarlar:

Onur Bender | Arzu Atalay

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.173-179

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346170

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Chlorogenic acid CGA is a major polyphenol in primary human diet, displaying a wide range of biological activities such as anti-oxidant, anti-inflammatory and anti-cancer effects. Several studies reported the chemopreventive effects of CGA on different types of cancers including breast cancer, which is the most common cancer among women worldwide. In this comprehensive study, we determined the anti-proliferative and cytotoxic effects of pure CGA on the phenotype of various breast cancer cell lines MCF-7, SKBR-3, MDA-MB-231, MDA-MB-468 and BT20 with well-defined molecular classification and characteristics, in a time and dose dependent manner by using iCELLigence real-time and label-free cell analysis technology. Cells were plated on iCELLigence system-specific E-plate L8 and treated with CGA for 72 hours at concentrations ranging from 250 μM to 8 mM. Obtained data were analyzed by RTCA data analysis software 1.0 and IC50 values of 95232.5 μM for MCF-7, 94021.2 μM for SKBR-3, 590.510.6 μM for MDA-MB-231, 882.512.0 μM for MDA-MB-468 and 1095121.6 μM for BT-20 cell lines were calculated at the end of 72-hour assay. Based on our findings, CGA did not have cytotoxic activity on breast cancer cell lines, and IC50 values and growth curves displayed similar anti-proliferative patterns with slight variation.

 Chlorogenic acid CGA is a major polyphenol in primary human diet, displaying a wide range of biological activities such as anti-oxidant, anti-inflammatory and anti-cancer effects. Several studies reported the chemopreventive effects of CGA on different types of cancers including breast cancer, which is the most common cancer among women worldwide. In this comprehensive study, we determined the anti-proliferative and cytotoxic effects of pure CGA on the phenotype of various breast cancer cell lines MCF-7, SKBR-3, MDA-MB-231, MDA-MB-468 and BT20 with well-defined molecular classification and characteristics, in a time and dose dependent manner by using iCELLigence real-time and label-free cell analysis technology. Cells were plated on iCELLigence system-specific E-plate L8 and treated with CGA for 72 hours at concentrations ranging from 250 μM to 8 mM. Obtained data were analyzed by RTCA data analysis software 1.0 and IC50 values of 95232.5 μM for MCF-7, 94021.2 μM for SKBR-3, 590.510.6 μM for MDA-MB-231, 882.512.0 μM for MDA-MB-468 and 1095121.6 μM for BT-20 cell lines were calculated at the end of 72-hour assay. Based on our findings, CGA did not have cytotoxic activity on breast cancer cell lines, and IC50 values and growth curves displayed similar anti-proliferative patterns with slight variation.

 Chlorogenic acid CGA is a major polyphenol in primary human diet, displaying a wide range of biological activities such as anti-oxidant, anti-inflammatory and anti-cancer effects. Several studies reported the chemopreventive effects of CGA on different types of cancers including breast cancer, which is the most common cancer among women worldwide. In this comprehensive study, we determined the anti-proliferative and cytotoxic effects of pure CGA on the phenotype of various breast cancer cell lines MCF-7, SKBR-3, MDA-MB-231, MDA-MB-468 and BT20 with well-defined molecular classification and characteristics, in a time and dose dependent manner by using iCELLigence real-time and label-free cell analysis technology. Cells were plated on iCELLigence system-specific E-plate L8 and treated with CGA for 72 hours at concentrations ranging from 250 μM to 8 mM. Obtained data were analyzed by RTCA data analysis software 1.0 and IC50 values of 95232.5 μM for MCF-7, 94021.2 μM for SKBR-3, 590.510.6 μM for MDA-MB-231, 882.512.0 μM for MDA-MB-468 and 1095121.6 μM for BT-20 cell lines were calculated at the end of 72-hour assay. Based on our findings, CGA did not have cytotoxic activity on breast cancer cell lines, and IC50 values and growth curves displayed similar anti-proliferative patterns with slight variation.

 Chlorogenic acid CGA is a major polyphenol in primary human diet, displaying a wide range of biological activities such as anti-oxidant, anti-inflammatory and anti-cancer effects. Several studies reported the chemopreventive effects of CGA on different types of cancers including breast cancer, which is the most common cancer among women worldwide. In this comprehensive study, we determined the anti-proliferative and cytotoxic effects of pure CGA on the phenotype of various breast cancer cell lines MCF-7, SKBR-3, MDA-MB-231, MDA-MB-468 and BT20 with well-defined molecular classification and characteristics, in a time and dose dependent manner by using iCELLigence real-time and label-free cell analysis technology. Cells were plated on iCELLigence system-specific E-plate L8 and treated with CGA for 72 hours at concentrations ranging from 250 μM to 8 mM. Obtained data were analyzed by RTCA data analysis software 1.0 and IC50 values of 95232.5 μM for MCF-7, 94021.2 μM for SKBR-3, 590.510.6 μM for MDA-MB-231, 882.512.0 μM for MDA-MB-468 and 1095121.6 μM for BT-20 cell lines were calculated at the end of 72-hour assay. Based on our findings, CGA did not have cytotoxic activity on breast cancer cell lines, and IC50 values and growth curves displayed similar anti-proliferative patterns with slight variation.

Makale detayları

Evaluation of anti-proliferative and cytotoxic effects of chlorogenic acid on breast cancer cell lines by real-time, label-free and high-throughput screening

Yazarlar:

Onur Bender | Arzu Atalay

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.173-179

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346170

pISSN:

2630-6344

chlorogenic acid

phenolic compound

natural extract

breast cancer

proliferation

cytotoxicity

xCELLigence

RTCA

Sayı

Makale detayları

Yazarlar: Onur Bender | Arzu Atalay

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.22 Issue 2, pp.173-179

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346170

pISSN: 2630-6344

Sayı

Yazarlar:

Onur Bender | Arzu Atalay

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.173-179

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346170

pISSN:

2630-6344