Immunohistochemical Expression of IL-2 and IL-4 in Thymoquinone Treated Rat Stomach and Anti-inflammatory Effect

Şerife Tütüncü; Tuğrul Ertuğrul; Nurcan Delice; Bengül Özdemir

Makale detayları

Immunohistochemical Expression of IL-2 and IL-4 in Thymoquinone Treated Rat Stomach and Anti-inflammatory Effect

Yazarlar:

Şerife Tütüncü | Tuğrul Ertuğrul | Nurcan Delice | Bengül Özdemir

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.15-21

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001326431

pISSN:

2667-6745

Anahtar kelimeler:

Özet:

 N. Sativa, a botanical specimen with an extensive medical history, has been historically employed by ancient Egyptian and Greek medical doctors to alleviate diverse afflictions and maladies. The plant contains a key bioactive component referred to as thymoquinone. Our study aims to assess the potential effects of thymoquinone, which impacts various diseases, on the stomach following oral gavage administration at different dosages. This assessment will be conducted in vivo and employ histochemical and immunohistochemical methods. A total of 21 rats of the Sprague-Dawley strain were used as the study material. The rats were divided into three groups: Experimental Group 1 10 mgkg gavage, Experimental Group 2 20 mgkg gavage, and the control group, each consisting of seven rats. Thymoquinone was administered to the experimental groups via oral gavage at 10 mgkg and 20 mgkg daily for forty-two days. As a result of this study demonstrated the expression of anti-inflammatory cytokines such as IL-2 and IL-4, which are effective in the immune system in the stomach in vivo. Distinct levels of immune reactions have been observed in the gastric mucosae of all groups of rats. Thus, it has been concluded that different doses of thymoquinone do not render cytokines inactive; however, variations are contingent upon the type and dosage of cytokine.

 N. Sativa, a botanical specimen with an extensive medical history, has been historically employed by ancient Egyptian and Greek medical doctors to alleviate diverse afflictions and maladies. The plant contains a key bioactive component referred to as thymoquinone. Our study aims to assess the potential effects of thymoquinone, which impacts various diseases, on the stomach following oral gavage administration at different dosages. This assessment will be conducted in vivo and employ histochemical and immunohistochemical methods. A total of 21 rats of the Sprague-Dawley strain were used as the study material. The rats were divided into three groups: Experimental Group 1 10 mgkg gavage, Experimental Group 2 20 mgkg gavage, and the control group, each consisting of seven rats. Thymoquinone was administered to the experimental groups via oral gavage at 10 mgkg and 20 mgkg daily for forty-two days. As a result of this study demonstrated the expression of anti-inflammatory cytokines such as IL-2 and IL-4, which are effective in the immune system in the stomach in vivo. Distinct levels of immune reactions have been observed in the gastric mucosae of all groups of rats. Thus, it has been concluded that different doses of thymoquinone do not render cytokines inactive; however, variations are contingent upon the type and dosage of cytokine.

 N. Sativa, a botanical specimen with an extensive medical history, has been historically employed by ancient Egyptian and Greek medical doctors to alleviate diverse afflictions and maladies. The plant contains a key bioactive component referred to as thymoquinone. Our study aims to assess the potential effects of thymoquinone, which impacts various diseases, on the stomach following oral gavage administration at different dosages. This assessment will be conducted in vivo and employ histochemical and immunohistochemical methods. A total of 21 rats of the Sprague-Dawley strain were used as the study material. The rats were divided into three groups: Experimental Group 1 10 mgkg gavage, Experimental Group 2 20 mgkg gavage, and the control group, each consisting of seven rats. Thymoquinone was administered to the experimental groups via oral gavage at 10 mgkg and 20 mgkg daily for forty-two days. As a result of this study demonstrated the expression of anti-inflammatory cytokines such as IL-2 and IL-4, which are effective in the immune system in the stomach in vivo. Distinct levels of immune reactions have been observed in the gastric mucosae of all groups of rats. Thus, it has been concluded that different doses of thymoquinone do not render cytokines inactive; however, variations are contingent upon the type and dosage of cytokine.

 Nigella sativa N. Sativa, eski Mısır ve Yunan hekimleri tarafından çeşitli ağrılar ve hastalıklarda kullanılan, uzun tıbbi geçmişe sahip olan bir bitkidir. Bitkinin asıl etken maddesi ise thmoquinone adı verilen bir maddedir. Çalışmamız, farklı hastalıklar üzerine etkileri olan timokinonun, oral gavaj yoluyla farklı dozlarda uygulanmasının ardından mideye olası etkilerini in vivo olarak değerlendirmeyi amaçlamaktadır. Bu değerlendirme histokimyasal ve immunohistokimyasal yöntemlerle gerçekleştirilecektir. Çalışma materyali olarak Sprague Dawley soyundan 21 adet sıçan kullanıldı. Sıçanlar, deney grubu 1 10 mgkg gavaj, deney grubu 2 20 mgkg gavaj ve kontrol grubu olmak üzere yedi sıçandan oluşan üçer gruba ayrıldı. Deney gruplarına kırk iki gün boyunca her gün 10 mgkg ve 20 mgkg dozlarda timokinon oral gavaj ile uygulanmıştır. Mevcut çalışma sonucunda immun sistemde etkili olan antienflamatuar sitokinlerden IL-2 ve IL-4ün midedeki ekspresyonları in vivo olarak gösterilmiştir. Tüm gruplardaki sıçanların mide mukozalarında farklı şiddetlerde immun reaksiyonlar gözlenmiştir. Böylelikle timokinonun farklı dozlarının sitokinleri inaktive etmediği ancak sitokin türüne ve doza bağlı olarak farklılıklar olduğu sonucuna varılmıştır.

Makale detayları

Immunohistochemical Expression of IL-2 and IL-4 in Thymoquinone Treated Rat Stomach and Anti-inflammatory Effect

Yazarlar:

Şerife Tütüncü | Tuğrul Ertuğrul | Nurcan Delice | Bengül Özdemir

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.15-21

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001326431

pISSN:

2667-6745

Cytokine

Immunohistochemistry

Inflammation

Rat

Stomachİmmunohistokimya

İnflamasyon

Mide

Sıçan

Sitokin

Timokinon

Sayı

Makale detayları

Yazarlar: Şerife Tütüncü | Tuğrul Ertuğrul | Nurcan Delice | Bengül Özdemir

Yayıncı: Bursa Uludağ University

Ana Başlıklar: Veterinary Sciences (Other)

Dergi: Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.15-21

Belge Tipi: Makale

Yayın Tarihi: 2/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001326431

pISSN: 2667-6745

Sayı

Yazarlar:

Şerife Tütüncü | Tuğrul Ertuğrul | Nurcan Delice | Bengül Özdemir

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Veterinary Sciences (Other)

Dergi:

Journal of Research in Veterinary Medicine Vol.43 Issue 1, pp.15-21

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001326431

pISSN:

2667-6745