INVESTIGATION OF HLA-B*51 SUB-ALLELES IN HLA-B*51 POSITIVE BEHCET PATIENTS

Sinem Topcu Ayas; Aslı Ozkızılcık Kocyıgıt; Tulay Kılıcaslan Ayna; Kiymet Handan Kelekci; Mustafa Soyoz; Ibrahim Pırım

Makale detayları

INVESTIGATION OF HLA-B51 SUB-ALLELES IN HLA-B51 POSITIVE BEHCET PATIENTS

Yazarlar:

Sinem Topcu Ayas | Aslı Ozkızılcık Kocyıgıt | Tulay Kılıcaslan Ayna | Kiymet Handan Kelekci | Mustafa Soyoz | Ibrahim Pırım

Yayıncı:

Izmir Katip Celebi University

Ana Başlıklar:

Epidemiyoloji (Diğer)

Dergi:

Güncel Tıbbi Araştırmaları Dergisi Vol.3 Issue 3, pp.6-16

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001308444

pISSN:

2791-7061

Anahtar kelimeler:

Özet:

 Aim: The initial identification of Behçets disease BD occurred in 1937, credited to the Turkish dermatologist Prof. Dr. Hulusi Behçet. Symptoms included repeated oral aphthae, genital ulcers, and uveitis. Then, it was revealed that the illness involves the joints, blood vessels, intestines, lungs, and brain system. Age, gender, psychological factors, infectious agents, immune responses, and genetic predispositions affect illness progression. The most common genetic risk factor is HLA-B51. This study aimed to examine the relationship between HLA-B51 sub-alleles and illness etiology. Materials and Method: We compared the prevalence of two HLA-B51 sub-alleles HLA-B51:01 and HLA-B51:08 in patients with BD versus healthy individuals. The samples of 24 HLA-B51 positive individuals were typed by Polymerase Chain Reaction with Sequence-Specific Primers PCR-SSP. In the control group, 73 healthy bone marrow donors were HLA-B51 subtyped by DNA sequencing. Results: The prevalence of HLA-B51:01 and 51:08 sub-alleles in patients was 80 and 20, respectively. The frequencies of HLA-B51:01, 02, 05, 07, and 08 in the control group were 90.4, 4.1, 2.7, 1.4, and 1.4, respectively. There was no significant difference in HLA-B51:01 allele frequency between patient and control groups p0.05; p0.457 RR1. Statistically significant differences were seen for the HLA-B51:08 allele p0.05; p0.003 RR18.8. No statistically significant correlation was found between HLA-B51:01 and HLA-B51:08 sub-alleles and clinical symptoms p-value  0.05. Conclusion: HLA-B51:08 sub-allele may be an important risk factor for BD development. Future investigations can further highlight the significance of its role in the pathophysiology of the disease.

 Aim: The initial identification of Behçets disease BD occurred in 1937, credited to the Turkish dermatologist Prof. Dr. Hulusi Behçet. Symptoms included repeated oral aphthae, genital ulcers, and uveitis. Then, it was revealed that the illness involves the joints, blood vessels, intestines, lungs, and brain system. Age, gender, psychological factors, infectious agents, immune responses, and genetic predispositions affect illness progression. The most common genetic risk factor is HLA-B51. This study aimed to examine the relationship between HLA-B51 sub-alleles and illness etiology. Materials and Method: We compared the prevalence of two HLA-B51 sub-alleles HLA-B51:01 and HLA-B51:08 in patients with BD versus healthy individuals. The samples of 24 HLA-B51 positive individuals were typed by Polymerase Chain Reaction with Sequence-Specific Primers PCR-SSP. In the control group, 73 healthy bone marrow donors were HLA-B51 subtyped by DNA sequencing. Results: The prevalence of HLA-B51:01 and 51:08 sub-alleles in patients was 80 and 20, respectively. The frequencies of HLA-B51:01, 02, 05, 07, and 08 in the control group were 90.4, 4.1, 2.7, 1.4, and 1.4, respectively. There was no significant difference in HLA-B51:01 allele frequency between patient and control groups p0.05; p0.457 RR1. Statistically significant differences were seen for the HLA-B51:08 allele p0.05; p0.003 RR18.8. No statistically significant correlation was found between HLA-B51:01 and HLA-B51:08 sub-alleles and clinical symptoms p-value  0.05. Conclusion: HLA-B51:08 sub-allele may be an important risk factor for BD development. Future investigations can further highlight the significance of its role in the pathophysiology of the disease.

 Aim: The initial identification of Behçets disease BD occurred in 1937, credited to the Turkish dermatologist Prof. Dr. Hulusi Behçet. Symptoms included repeated oral aphthae, genital ulcers, and uveitis. Then, it was revealed that the illness involves the joints, blood vessels, intestines, lungs, and brain system. Age, gender, psychological factors, infectious agents, immune responses, and genetic predispositions affect illness progression. The most common genetic risk factor is HLA-B51. This study aimed to examine the relationship between HLA-B51 sub-alleles and illness etiology. Materials and Method: We compared the prevalence of two HLA-B51 sub-alleles HLA-B51:01 and HLA-B51:08 in patients with BD versus healthy individuals. The samples of 24 HLA-B51 positive individuals were typed by Polymerase Chain Reaction with Sequence-Specific Primers PCR-SSP. In the control group, 73 healthy bone marrow donors were HLA-B51 subtyped by DNA sequencing. Results: The prevalence of HLA-B51:01 and 51:08 sub-alleles in patients was 80 and 20, respectively. The frequencies of HLA-B51:01, 02, 05, 07, and 08 in the control group were 90.4, 4.1, 2.7, 1.4, and 1.4, respectively. There was no significant difference in HLA-B51:01 allele frequency between patient and control groups p0.05; p0.457 RR1. Statistically significant differences were seen for the HLA-B51:08 allele p0.05; p0.003 RR18.8. No statistically significant correlation was found between HLA-B51:01 and HLA-B51:08 sub-alleles and clinical symptoms p-value  0.05. Conclusion: HLA-B51:08 sub-allele may be an important risk factor for BD development. Future investigations can further highlight the significance of its role in the pathophysiology of the disease.

 Amaç: 1937 yılında Türk Dermatolog Prof. Dr. Hulusi Behçet tarafından keşfedilen Behçet Hastalığının BH semptomları tekrar eden oral aft, genital ülserler ve üveyitti. Daha sonra bu hastalığın eklemleri, kan damarlarını, bağırsakları, akciğerleri ve beyin sistemini de kapsadığı bildirilmiştir. Hastalığın seyrini yaş, cinsiyet, fizyolojik faktörler, enfeksiyöz ajanlar, immün yanıt ve genetik geçişler etkiler. En yaygın görülen genetik risk faktörü İnsan Lökosit Antijeni HLA-B51dir. Bu çalışmada HLA-B51 alt alleleri ile hastalık etyolojisi arasındaki ilişki irdelenmesi amaçlanmıştır. Gereç ve Yöntem: BH hastaları ile kontrol grubu arasında HLA-B51 alt allellerinin prevalanslarını karşılaştırdık. Sekans spesifik primer polimeraz zincir reaksiyonu SSP-PZR yöntemiyle HLA-B51 pozitif olan 24 kişinin doku tiplemesi yapılmıştır. Kontrol grubunda ise kemik iliği donörü olan 73 sağlıklı bireyin HLA-B51 alt allel tiplemesi DNA dizi analizi yöntemiyle belirlenmiştir. Bulgular: Hasta grubunda HLA-B51:01 ve 51:08 alt allellerinin prevalansı sırasıyla 80 ve 20 bulunmuştur. HLA-B51:01, 02, 05, 07 ve 08 alt allellerinin kontrol grubundaki sıklıkları sırasıyla 90,4, 4,1, 2,7 1,4 ve 1,4 olarak bulunmuştur. Hasta ve control grubu arasında HLA-B51:01 alt allelinin sıklığı bakımından istatistiksel olarak anlamlı bir farklılık bulunmamıştır p0.05; p0.457 RR1. İstatistiksel olarak anlamlı sonuçlar HLA-B51:08 alt alleli için bulunmuştur p0.05; p0.003 RR18.8. HLA-B51:01 ve HLA-B51:08 alt allelleri ile klinik semptomlar arasında anlamlı bir ilişki bulunmamıştır. Sonuç: HLA-B51:08 alt allelinin BH gelişiminde önemli bir faktör olabilir. Yapılacak çalışmalarla hastalığın patofizyolojisi açısından önemi gösterilebilir.

Makale detayları

INVESTIGATION OF HLA-B51 SUB-ALLELES IN HLA-B51 POSITIVE BEHCET PATIENTS

Yazarlar:

Sinem Topcu Ayas | Aslı Ozkızılcık Kocyıgıt | Tulay Kılıcaslan Ayna | Kiymet Handan Kelekci | Mustafa Soyoz | Ibrahim Pırım

Yayıncı:

Izmir Katip Celebi University

Ana Başlıklar:

Epidemiyoloji (Diğer)

Dergi:

Güncel Tıbbi Araştırmaları Dergisi Vol.3 Issue 3, pp.6-16

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001308444

pISSN:

2791-7061

Behçet’s disease

HLA-B*51 sub-alleles

organ involvement

PCR

geneBehçet hastalığı

HLA-B*51 alt alleli

organ tutulumu

PZR

gen

Sayı

Makale detayları

Yazarlar: Sinem Topcu Ayas | Aslı Ozkızılcık Kocyıgıt | Tulay Kılıcaslan Ayna | Kiymet Handan Kelekci | Mustafa Soyoz | Ibrahim Pırım

Yayıncı: Izmir Katip Celebi University

Ana Başlıklar: Epidemiyoloji (Diğer)

Dergi: Güncel Tıbbi Araştırmaları Dergisi Vol.3 Issue 3, pp.6-16

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001308444

pISSN: 2791-7061

Sayı

Yazarlar:

Sinem Topcu Ayas | Aslı Ozkızılcık Kocyıgıt | Tulay Kılıcaslan Ayna | Kiymet Handan Kelekci | Mustafa Soyoz | Ibrahim Pırım

Yayıncı:

Izmir Katip Celebi University

Ana Başlıklar:

Epidemiyoloji (Diğer)

Dergi:

Güncel Tıbbi Araştırmaları Dergisi Vol.3 Issue 3, pp.6-16

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001308444

pISSN:

2791-7061