The Effects of Angiotensin II, Olmesartan and PD123,319 on Protein Kinase C in STZ Induced Rat Vascular Smooth Muscle Cell Culture

Zehra Çiçek; Kübra Akıllıoğlu; Ayşe Doğan

Makale detayları

The Effects of Angiotensin II, Olmesartan and PD123,319 on Protein Kinase C in STZ Induced Rat Vascular Smooth Muscle Cell Culture

Yazarlar:

Zehra Çiçek | Kübra Akıllıoğlu | Ayşe Doğan

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Black Sea Journal of Health Science Vol.6 Issue 1, pp.127-133

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281467

pISSN:

2619-9041

Anahtar kelimeler:

Özet:

 Protein kinase C PKC is a promoter enzyme that plays a vital role in signal transduction of vascular smooth muscle cells VSMCs. It has numerous vascular functions, such as vascular cell growth, cytokine activation and angiogenesis. But, these mechanisms are deteriorating in diabetes mellitus. Angiotensin II Ang II effects on vascular structure binding Ang II type-1 and type-2 receptors ATR1 and ATR2 and stimulates PKC mostly through ATR1s. We aim to investigate the effects of Ang II, Olmesartan and PD123,319 on PKC levels of healthy and streptozotocin STZ induced rat VSMCs H-VSMCs and STZ-VSMCs in this study. The primary culture of VSMCs were isolated from thoracic aorta of healthy and STZ 45 mgkg, given via the tail vein induced Wistar rats. PKC levels were measured in H-VSMCs and STZ-VSMCs by enzyme-linked immunosorbent assay ELISA. In H-VSMCs, Ang II group compared to the control group, PKC levels decreased significantly P  0.000; whereas, Ang II group compared to control, PKC levels were higher, but not significantly in STZ-VSMCs P  0.088. PKC levels were increased in Ang IIOlmesartan P  0.000 and Ang IIPD123,319 P  0.000 groups compared to Ang II group in H-VSMCs, but in STZ-VSMCs, PKC levels in Ang IIOlmesartan P  0.001 and Ang IIPD123,319 P  0.000 groups compared to Ang II group were decreased significantly. Ang II, its receptors and PKC seem to modulate each other and may have a relationship in hyperglycemic conditions. Also, considering that, ATR1 blocker ATR1B Olmesartan and ATR2 blocker ATR2B PD123,319 may be protective against vascular injury by reducing PKC levels in STZ-VSMCs.

 Protein kinase C PKC is a promoter enzyme that plays a vital role in signal transduction of vascular smooth muscle cells VSMCs. It has numerous vascular functions, such as vascular cell growth, cytokine activation and angiogenesis. But, these mechanisms are deteriorating in diabetes mellitus. Angiotensin II Ang II effects on vascular structure binding Ang II type-1 and type-2 receptors ATR1 and ATR2 and stimulates PKC mostly through ATR1s. We aim to investigate the effects of Ang II, Olmesartan and PD123,319 on PKC levels of healthy and streptozotocin STZ induced rat VSMCs H-VSMCs and STZ-VSMCs in this study. The primary culture of VSMCs were isolated from thoracic aorta of healthy and STZ 45 mgkg, given via the tail vein induced Wistar rats. PKC levels were measured in H-VSMCs and STZ-VSMCs by enzyme-linked immunosorbent assay ELISA. In H-VSMCs, Ang II group compared to the control group, PKC levels decreased significantly P  0.000; whereas, Ang II group compared to control, PKC levels were higher, but not significantly in STZ-VSMCs P  0.088. PKC levels were increased in Ang IIOlmesartan P  0.000 and Ang IIPD123,319 P  0.000 groups compared to Ang II group in H-VSMCs, but in STZ-VSMCs, PKC levels in Ang IIOlmesartan P  0.001 and Ang IIPD123,319 P  0.000 groups compared to Ang II group were decreased significantly. Ang II, its receptors and PKC seem to modulate each other and may have a relationship in hyperglycemic conditions. Also, considering that, ATR1 blocker ATR1B Olmesartan and ATR2 blocker ATR2B PD123,319 may be protective against vascular injury by reducing PKC levels in STZ-VSMCs.

 Protein kinase C PKC is a promoter enzyme that plays a vital role in signal transduction of vascular smooth muscle cells VSMCs. It has numerous vascular functions, such as vascular cell growth, cytokine activation and angiogenesis. But, these mechanisms are deteriorating in diabetes mellitus. Angiotensin II Ang II effects on vascular structure binding Ang II type-1 and type-2 receptors ATR1 and ATR2 and stimulates PKC mostly through ATR1s. We aim to investigate the effects of Ang II, Olmesartan and PD123,319 on PKC levels of healthy and streptozotocin STZ induced rat VSMCs H-VSMCs and STZ-VSMCs in this study. The primary culture of VSMCs were isolated from thoracic aorta of healthy and STZ 45 mgkg, given via the tail vein induced Wistar rats. PKC levels were measured in H-VSMCs and STZ-VSMCs by enzyme-linked immunosorbent assay ELISA. In H-VSMCs, Ang II group compared to the control group, PKC levels decreased significantly P  0.000; whereas, Ang II group compared to control, PKC levels were higher, but not significantly in STZ-VSMCs P  0.088. PKC levels were increased in Ang IIOlmesartan P  0.000 and Ang IIPD123,319 P  0.000 groups compared to Ang II group in H-VSMCs, but in STZ-VSMCs, PKC levels in Ang IIOlmesartan P  0.001 and Ang IIPD123,319 P  0.000 groups compared to Ang II group were decreased significantly. Ang II, its receptors and PKC seem to modulate each other and may have a relationship in hyperglycemic conditions. Also, considering that, ATR1 blocker ATR1B Olmesartan and ATR2 blocker ATR2B PD123,319 may be protective against vascular injury by reducing PKC levels in STZ-VSMCs.

 Protein kinase C PKC is a promoter enzyme that plays a vital role in signal transduction of vascular smooth muscle cells VSMCs. It has numerous vascular functions, such as vascular cell growth, cytokine activation and angiogenesis. But, these mechanisms are deteriorating in diabetes mellitus. Angiotensin II Ang II effects on vascular structure binding Ang II type-1 and type-2 receptors ATR1 and ATR2 and stimulates PKC mostly through ATR1s. We aim to investigate the effects of Ang II, Olmesartan and PD123,319 on PKC levels of healthy and streptozotocin STZ induced rat VSMCs H-VSMCs and STZ-VSMCs in this study. The primary culture of VSMCs were isolated from thoracic aorta of healthy and STZ 45 mgkg, given via the tail vein induced Wistar rats. PKC levels were measured in H-VSMCs and STZ-VSMCs by enzyme-linked immunosorbent assay ELISA. In H-VSMCs, Ang II group compared to the control group, PKC levels decreased significantly P  0.000; whereas, Ang II group compared to control, PKC levels were higher, but not significantly in STZ-VSMCs P  0.088. PKC levels were increased in Ang IIOlmesartan P  0.000 and Ang IIPD123,319 P  0.000 groups compared to Ang II group in H-VSMCs, but in STZ-VSMCs, PKC levels in Ang IIOlmesartan P  0.001 and Ang IIPD123,319 P  0.000 groups compared to Ang II group were decreased significantly. Ang II, its receptors and PKC seem to modulate each other and may have a relationship in hyperglycemic conditions. Also, considering that, ATR1 blocker ATR1B Olmesartan and ATR2 blocker ATR2B PD123,319 may be protective against vascular injury by reducing PKC levels in STZ-VSMCs.

Makale detayları

The Effects of Angiotensin II, Olmesartan and PD123,319 on Protein Kinase C in STZ Induced Rat Vascular Smooth Muscle Cell Culture

Yazarlar:

Zehra Çiçek | Kübra Akıllıoğlu | Ayşe Doğan

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Black Sea Journal of Health Science Vol.6 Issue 1, pp.127-133

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281467

pISSN:

2619-9041

Ang II

PKC

Vascular smooth muscle

Cell culture

Ang II

PKC

Vascular smooth muscle

Cell culture

Sayı

Makale detayları

Yazarlar: Zehra Çiçek | Kübra Akıllıoğlu | Ayşe Doğan

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Klinik Tıp Bilimleri

Dergi: Black Sea Journal of Health Science Vol.6 Issue 1, pp.127-133

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001281467

pISSN: 2619-9041

Sayı

Yazarlar:

Zehra Çiçek | Kübra Akıllıoğlu | Ayşe Doğan

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Black Sea Journal of Health Science Vol.6 Issue 1, pp.127-133

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281467

pISSN:

2619-9041