EFFECT OF CHRONIC STRESS ON SERUM AND TISSUE LEVELS OF KLOTHO AND TNF-α

Mehmet Deniz Yener; Tuncay Çolak; Esra Acar; Fatma Ceyla Eraldemir

Makale detayları

EFFECT OF CHRONIC STRESS ON SERUM AND TISSUE LEVELS OF KLOTHO AND TNF-α

Yazarlar:

Mehmet Deniz Yener | Tuncay Çolak | Esra Acar | Fatma Ceyla Eraldemir

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Journal of Advanced Research in Health Sciences Vol.7 Issue 2, pp.117-123

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330843

pISSN:

2651-4060

Anahtar kelimeler:

Özet:

 Objective: Chronic stress is recognized as a factor that affects almost all organs and tissues and disrupts homeostasis. Stress also directly impacts the inflammatory process. In addition, klotho is a protein associated with lifespan. This study investigates the relationship that chronic stress has with klotho and tumor necrosis factor alpha TNF-α, which is a key protein in the proinflammatory process. Materials and Methods: The study involves 16 Wistar albino rats divided into control and stress groups. The study applies the protocol for the chronic unpredictable mild stress CUMS model and uses the enzymelinked immunosorbent assay ELISA method to measure the TNF-α and Klotho levels in the serum, heart, aorta, and liver tissues. Results: The serum samples obtained from the stress group were found to have a significant decrease in klotho levels p0.013 and high TNF-α levels p0.042. However, no significant difference was observed in the TNF-α or klotho levels in the heart, aorta, and liver tissues between groups. Conclusion: Under chronic stress, a significant increase is observed in TNFα, one of the main cytokines of inflammation, as well as a decrease in klotho hormone. This research endeavors to contribute to the existing body of knowledge concerning the interplay among stress, inflammation, and longevity.

 Objective: Chronic stress is recognized as a factor that affects almost all organs and tissues and disrupts homeostasis. Stress also directly impacts the inflammatory process. In addition, klotho is a protein associated with lifespan. This study investigates the relationship that chronic stress has with klotho and tumor necrosis factor alpha TNF-α, which is a key protein in the proinflammatory process. Materials and Methods: The study involves 16 Wistar albino rats divided into control and stress groups. The study applies the protocol for the chronic unpredictable mild stress CUMS model and uses the enzymelinked immunosorbent assay ELISA method to measure the TNF-α and Klotho levels in the serum, heart, aorta, and liver tissues. Results: The serum samples obtained from the stress group were found to have a significant decrease in klotho levels p0.013 and high TNF-α levels p0.042. However, no significant difference was observed in the TNF-α or klotho levels in the heart, aorta, and liver tissues between groups. Conclusion: Under chronic stress, a significant increase is observed in TNFα, one of the main cytokines of inflammation, as well as a decrease in klotho hormone. This research endeavors to contribute to the existing body of knowledge concerning the interplay among stress, inflammation, and longevity.

 Objective: Chronic stress is recognized as a factor that affects almost all organs and tissues and disrupts homeostasis. Stress also directly impacts the inflammatory process. In addition, klotho is a protein associated with lifespan. This study investigates the relationship that chronic stress has with klotho and tumor necrosis factor alpha TNF-α, which is a key protein in the proinflammatory process. Materials and Methods: The study involves 16 Wistar albino rats divided into control and stress groups. The study applies the protocol for the chronic unpredictable mild stress CUMS model and uses the enzymelinked immunosorbent assay ELISA method to measure the TNF-α and Klotho levels in the serum, heart, aorta, and liver tissues. Results: The serum samples obtained from the stress group were found to have a significant decrease in klotho levels p0.013 and high TNF-α levels p0.042. However, no significant difference was observed in the TNF-α or klotho levels in the heart, aorta, and liver tissues between groups. Conclusion: Under chronic stress, a significant increase is observed in TNFα, one of the main cytokines of inflammation, as well as a decrease in klotho hormone. This research endeavors to contribute to the existing body of knowledge concerning the interplay among stress, inflammation, and longevity.

 Amaç: Kronik stres hemen hemen tüm organlarıdokuları etkileyen ve homeostazı bozan bir faktör olarak kabul edilmektedir. Stres, enflamatuar süreç üzerinde doğrudan bir etkiye sahiptir. Bununla birlikte, Klotho yaşam süresi ile ilişkili bir proteindir. Çalışmamızda kronik stres ile proinflamatuar süreçte anahtar bir protein olan Tümör nekroz faktör alfa TNF-α ve Klotho arasındaki ilişkiyi araştırdık. Gereç ve Yöntemler: Çalışmamız kontrol ve stres gruplarına ayrılan 16 Wistar albinodan oluşmuştur. Kronik öngörülemeyen hafif stres model protokolü uygulandı. Serum, kalp, aort ve karaciğer dokusundaki TNF-α ve Klotho düzeyleri ELISA yöntemi kullanılarak ölçüldü. Bulgular: Stres grubundan alınan serum örneklerinin Klotho düzeylerinde anlamlı azalma p0,013, TNF-α düzeylerinde ise anlamlı yükseliş p0,042 olduğu belirlendi. Ancak; gruplar arasında kalp, aort ve karaciğer dokularındaki TNF-α ve Klotho düzeylerinde anlamlı bir fark gözlenmedi. Sonuç: Kronik stres altında inflamasyonun ana sitokinlerinden biri olan TNF-α da belirgin bir artış, Klotho hormonunda ise azalma gözlendi. Araştırmamız; stres, iltihaplanma ve uzun ömür arasındaki etkileşime ilişkin mevcut bilgi birikimine katkıda bulunmaya çalışmaktadır.

Makale detayları

EFFECT OF CHRONIC STRESS ON SERUM AND TISSUE LEVELS OF KLOTHO AND TNF-α

Yazarlar:

Mehmet Deniz Yener | Tuncay Çolak | Esra Acar | Fatma Ceyla Eraldemir

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Journal of Advanced Research in Health Sciences Vol.7 Issue 2, pp.117-123

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330843

pISSN:

2651-4060

Chronic stress

inflammation

klotho

TNF-αKronik stres

inflamasyon

Klotho

TNF-α

Sayı

Makale detayları

Yazarlar: Mehmet Deniz Yener | Tuncay Çolak | Esra Acar | Fatma Ceyla Eraldemir

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences (Other)

Dergi: Journal of Advanced Research in Health Sciences Vol.7 Issue 2, pp.117-123

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001330843

pISSN: 2651-4060

Sayı

Yazarlar:

Mehmet Deniz Yener | Tuncay Çolak | Esra Acar | Fatma Ceyla Eraldemir

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Journal of Advanced Research in Health Sciences Vol.7 Issue 2, pp.117-123

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330843

pISSN:

2651-4060