DNA Barcoding of Docotettix cornutus Ribaut, 1948 (Hemiptera: Cicadellidae)

Eyyüp Mennan Yıldırım

Makale detayları

DNA Barcoding of Docotettix cornutus Ribaut, 1948 (Hemiptera: Cicadellidae)

Yazarlar:

Eyyüp Mennan Yıldırım

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.21 Issue 2, pp.173-176

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001338177

pISSN:

1304-7787

Anahtar kelimeler:

Özet:

 Docotettix cornutus Ribaut 1948 Hemiptera: Cicadellidae is a species that can cause economic damage to crops such as tomato, olive, pomegranate, and maize and is distributed worldwide. Since morphological identification is very difficult due to the small size of this pest there is a lack of experts and no record of molecular identification, DNA barcoding was aimed. In this study, D. cornutus was collected from pomegranate orchards in Aydın, İzmir and Manisa provinces Türkiye using Japanese umbrellas and suction tubes. After morphological identification of the species, HCO LCO universal primers were used to amplify the COI gene region of the specimen, and healthy reads were made between 499 bp SE05 and 675 bp SE07. Sequence analysis of the specimen was performed by Triogen Biotechnology Istanbul-Türkiye. D. cornutus was registered in Genebank for the first time and a GeneBank accession number was obtained. When the specimens were compared with other registered specimens in the BLAST module at NCBI, it was seen that the closest species to D. cornutus was Synophropsis lauri.

 Docotettix cornutus Ribaut 1948 Hemiptera: Cicadellidae is a species that can cause economic damage to crops such as tomato, olive, pomegranate, and maize and is distributed worldwide. Since morphological identification is very difficult due to the small size of this pest there is a lack of experts and no record of molecular identification, DNA barcoding was aimed. In this study, D. cornutus was collected from pomegranate orchards in Aydın, İzmir and Manisa provinces Türkiye using Japanese umbrellas and suction tubes. After morphological identification of the species, HCO LCO universal primers were used to amplify the COI gene region of the specimen, and healthy reads were made between 499 bp SE05 and 675 bp SE07. Sequence analysis of the specimen was performed by Triogen Biotechnology Istanbul-Türkiye. D. cornutus was registered in Genebank for the first time and a GeneBank accession number was obtained. When the specimens were compared with other registered specimens in the BLAST module at NCBI, it was seen that the closest species to D. cornutus was Synophropsis lauri.

 Docotettix cornutus Ribaut 1948 Hemiptera: Cicadellidae is a species that can cause economic damage to crops such as tomato, olive, pomegranate, and maize and is distributed worldwide. Since morphological identification is very difficult due to the small size of this pest there is a lack of experts and no record of molecular identification, DNA barcoding was aimed. In this study, D. cornutus was collected from pomegranate orchards in Aydın, İzmir and Manisa provinces Türkiye using Japanese umbrellas and suction tubes. After morphological identification of the species, HCO LCO universal primers were used to amplify the COI gene region of the specimen, and healthy reads were made between 499 bp SE05 and 675 bp SE07. Sequence analysis of the specimen was performed by Triogen Biotechnology Istanbul-Türkiye. D. cornutus was registered in Genebank for the first time and a GeneBank accession number was obtained. When the specimens were compared with other registered specimens in the BLAST module at NCBI, it was seen that the closest species to D. cornutus was Synophropsis lauri.

 Docotettix cornutus Ribaut 1948 Hemiptera: Cicadellidae domates, zeytin, nar ve mısır gibi bitkiler üzerinde ekonomik zarara neden olabilen ve tüm dünyada yayılış gösteren bir türdür. Morfolojik teşhisi zararlının küçük olması ve uzman eksikliği gibi nedenlerle oldukça zor yapılabilmekte olup, moleküler teşhisi ile ilgili herhangi bir kayıt da bulunmaması nedeniyle DNA barkodlamasının yapılması amaçlanmıştır. Çalışmada D. cornutus; Aydın, İzmir ve Manisa illerinde Türkiye nar bahçelerinden japon şemsiyesi ve emgi tüpü yardımıyla toplanmıştır. Morfolojik olarak tür teşhisi yapıldıktan sonra; örneklerin COI gen bölgesinin çoğaltılması için HCO LCO evrensel primerler kullanılmış ve 499bç SE05 ile 675bç SE07 arasında sağlıklı okuma yapılmıştır. Örneklerin sekans analizi Triogen Biyoteknoloji İstanbul-Türkiye tarafından yapılmıştır. D. cornutus ilk kez Genbanka kaydedilmiş ve Gen Bankası erişim numarası alınmıştır. Örnekler; NCBIda BLAST modülünde diğer kayıtlı örneklerle karşılaştırıldığında D. cornutus a en yakın türün Synophropsis lauri olduğu görülmüştür.

Makale detayları

DNA Barcoding of Docotettix cornutus Ribaut, 1948 (Hemiptera: Cicadellidae)

Yazarlar:

Eyyüp Mennan Yıldırım

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.21 Issue 2, pp.173-176

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001338177

pISSN:

1304-7787

COI

DNA barcoding

Docotettix cornutus

TurkiyeCOI

DNA barkodlama

Docotettix cornutus

Türkiye

Sayı

Makale detayları

Yazarlar: Eyyüp Mennan Yıldırım

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Ziraat Mühendisliği (Diğer)

Dergi: Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.21 Issue 2, pp.173-176

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001338177

pISSN: 1304-7787

Sayı

Yazarlar:

Eyyüp Mennan Yıldırım

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.21 Issue 2, pp.173-176

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001338177

pISSN:

1304-7787