Phenolic Profile and Bioactivity of Hazelnut Shell: Antioxidant, Anti-inflammatory and Enzyme Inhibition Potential

Adem Demir

Makale detayları

Phenolic Profile and Bioactivity of Hazelnut Shell: Antioxidant, Anti-inflammatory and Enzyme Inhibition Potential

Yazarlar:

Adem Demir

Yayıncı:

Recep Tayyip Erdoğan University

Ana Başlıklar:

Biyokataliz ve Enzim Teknolojisi | Enstrümantal Yöntemler | Doğal Ürünler ve Biyoaktif Bileşikler

Dergi:

Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 1, pp.298-316

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001351020

pISSN:

2687-2315

Anahtar kelimeler:

Özet:

 The conversion of agricultural bio-waste into high value-added products in the food and pharmaceutical industries is important in terms of environmental sustainability. Hazelnut shells are one of the main by-products of hazelnut processing, causing both economic and environmental problems. The phenolic composition of the ethanolic extract of hazelnut shells was analysed by High performance liquid chromatography- diode array dedector HPLC-DAD and the antioxidant, anti-inflammatory, urease and α-glycosidase enzyme inhibition capacities were investigated. The ethanol extract showed strong radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl DPPH and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid ABTS radicals with SC50 values of 20.2 µgmL and 32.8 µgmL, respectively, due to its high total polyphenol content 150.91 mg Gallic Acid Equivalent GAEg. In HPLC-DAD analysis, gallic acid was found to be the most abundant phenolic component with a concentration of 586.8 µgg, and the presence of protocatechuic acid, catechin, vanillic acid, caffeic acid, p-coumaric acid and quercetin was determined in the range of 124.5-421.5 µgg. It showed a significant anti-inflammatory effect by inhibiting protein denaturation with an IC₅₀ value of 346.4 µgmL. Inhibition activity was observed on urease and α-glucosidase enzymes with IC50 values of 24.7 µgmL and 194.4 µgmL, respectively. The results of the present study indicate that hazelnut shells represent a valuable resource in the food and pharmaceutical industries due to their abundant phenolic content and pronounced biological activities.

 The conversion of agricultural bio-waste into high value-added products in the food and pharmaceutical industries is important in terms of environmental sustainability. Hazelnut shells are one of the main by-products of hazelnut processing, causing both economic and environmental problems. The phenolic composition of the ethanolic extract of hazelnut shells was analysed by High performance liquid chromatography- diode array dedector HPLC-DAD and the antioxidant, anti-inflammatory, urease and α-glycosidase enzyme inhibition capacities were investigated. The ethanol extract showed strong radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl DPPH and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid ABTS radicals with SC50 values of 20.2 µgmL and 32.8 µgmL, respectively, due to its high total polyphenol content 150.91 mg Gallic Acid Equivalent GAEg. In HPLC-DAD analysis, gallic acid was found to be the most abundant phenolic component with a concentration of 586.8 µgg, and the presence of protocatechuic acid, catechin, vanillic acid, caffeic acid, p-coumaric acid and quercetin was determined in the range of 124.5-421.5 µgg. It showed a significant anti-inflammatory effect by inhibiting protein denaturation with an IC₅₀ value of 346.4 µgmL. Inhibition activity was observed on urease and α-glucosidase enzymes with IC50 values of 24.7 µgmL and 194.4 µgmL, respectively. The results of the present study indicate that hazelnut shells represent a valuable resource in the food and pharmaceutical industries due to their abundant phenolic content and pronounced biological activities.

 The conversion of agricultural bio-waste into high value-added products in the food and pharmaceutical industries is important in terms of environmental sustainability. Hazelnut shells are one of the main by-products of hazelnut processing, causing both economic and environmental problems. The phenolic composition of the ethanolic extract of hazelnut shells was analysed by High performance liquid chromatography- diode array dedector HPLC-DAD and the antioxidant, anti-inflammatory, urease and α-glycosidase enzyme inhibition capacities were investigated. The ethanol extract showed strong radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl DPPH and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid ABTS radicals with SC50 values of 20.2 µgmL and 32.8 µgmL, respectively, due to its high total polyphenol content 150.91 mg Gallic Acid Equivalent GAEg. In HPLC-DAD analysis, gallic acid was found to be the most abundant phenolic component with a concentration of 586.8 µgg, and the presence of protocatechuic acid, catechin, vanillic acid, caffeic acid, p-coumaric acid and quercetin was determined in the range of 124.5-421.5 µgg. It showed a significant anti-inflammatory effect by inhibiting protein denaturation with an IC₅₀ value of 346.4 µgmL. Inhibition activity was observed on urease and α-glucosidase enzymes with IC50 values of 24.7 µgmL and 194.4 µgmL, respectively. The results of the present study indicate that hazelnut shells represent a valuable resource in the food and pharmaceutical industries due to their abundant phenolic content and pronounced biological activities.

 Tarımsal biyoatıkların, gıda ve ilaç sanayisinde katma değeri yüksek ürünlere dönüştürülmesi, çevresel sürdürülebilirlik için büyük önem taşımaktadır. Fındık kabukları, fındığın işlenmesi sırasında ortaya çıkan ve hem ekonomik hem de çevresel sorunlara yol açan başlıca yan ürünlerden biridir. Fındık kabuğunun etanol ekstraktının yüksek performanslı sıvı kromatografisi-diyot dizi dedekör HPLC-DAD ile fenolik bileşimi incelenerek antioksidan, antiinflamatuvar, üreaz ve α-glukozidaz enzim inhibisyon kapasiteleri araştırılmıştır. Etanol ekstraktı, yüksek toplam polifenol içeriği 150,91 mg Gallik Asit Eşdeğer GAEg sayesinde 1,1-difenil-2-pikrilhidrazil DPPH ve 2,2-Azino-bis3-etilbenztiazolin-6-sülfonik asit ABTS radikallerine karşı sırasıyla 20,2 µgmL ve 32,8 µgmL SC50 değerleriyle güçlü radikal süpürme aktivitesi göstermiştir. HPLC-DAD analizinde, 586,8 µgg konsantrasyonla en bol bulunan fenolik bileşen gallik asit olarak tespit edilmiş; ayrıca 124,5-421,5 µgg aralığında protokatekuik asit, kateşin, vanilik asit, kafeik asit, p- kumarik asit ve kuersetin varlığı belirlenmiştir. Protein denatürasyonunu 346,4 µgmL IC₅₀ değeriyle inhibe ederek belirgin bir antiinflamatuvar etki göstermiştir. Üreaz ve α-glukozidaz enzimleri üzerinde sırasıyla 24,7 µgmL ve 194,4 µgmL IC50 değerleri ile inhibisyon aktivitesi gözlenmiştir. Bu sonuçlar, fındık kabuğunun zengin fenolik içeriği ve güçlü biyolojik aktiviteleri sayesinde gıda ve ilaç sanayisinde değerli bir kaynak olarak kullanılabileceğini ortaya koymaktadır.

Makale detayları

Phenolic Profile and Bioactivity of Hazelnut Shell: Antioxidant, Anti-inflammatory and Enzyme Inhibition Potential

Yazarlar:

Adem Demir

Yayıncı:

Recep Tayyip Erdoğan University

Ana Başlıklar:

Biyokataliz ve Enzim Teknolojisi | Enstrümantal Yöntemler | Doğal Ürünler ve Biyoaktif Bileşikler

Dergi:

Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 1, pp.298-316

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001351020

pISSN:

2687-2315

Hazelnut Shell

HPLC

BSA denaturation

urease

α-glycosidaseFındık kabuğu

HPLC

BSA Denatürasyonu

üreaz

α-glikozidaz

Sayı

Makale detayları

Yazarlar: Adem Demir

Yayıncı: Recep Tayyip Erdoğan University

Ana Başlıklar: Biyokataliz ve Enzim Teknolojisi | Enstrümantal Yöntemler | Doğal Ürünler ve Biyoaktif Bileşikler

Dergi: Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 1, pp.298-316

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001351020

pISSN: 2687-2315

Sayı

Yazarlar:

Adem Demir

Yayıncı:

Recep Tayyip Erdoğan University

Ana Başlıklar:

Biyokataliz ve Enzim Teknolojisi | Enstrümantal Yöntemler | Doğal Ürünler ve Biyoaktif Bileşikler

Dergi:

Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 1, pp.298-316

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001351020

pISSN:

2687-2315