Efficiency of using RAPD and ISSR markers in evaluation of genetic diversity in sugar beet

Vusala IZZATULLAYEVA; Zeynal AKPAROV; Sevda BABAYEVA; Javid OJAGHI; Mehraj ABBASOV

Makale detayları

Efficiency of using RAPD and ISSR markers in evaluation of genetic diversity in sugar beet

Yazarlar:

Vusala IZZATULLAYEVA | Zeynal AKPAROV | Sevda BABAYEVA | Javid OJAGHI | Mehraj ABBASOV

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.429-438

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812149

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers were used for comparative analysis of genetic variation in 42 sugar beet accessions. A total of 24 polymorphic primers (12 RAPD and 12 ISSR) were used. The RAPD primers generated 204 amplification products and the ISSR primers produced 178 fragments, 190 and 173 of which were polymorphic, respectively. The average polymorphism level with the ISSR markers (97.2%) was higher than that with the RAPD primers (93%). High genetic diversity indices for both marker types (0.86 for RAPD and 0.91 for ISSR) suggested that these methods were equally effective in determining genetic variation in sugar beet accessions. Cluster analysis of the RAPD, ISSR, and combined datasets revealed similar grouping patterns. However, the dendrogram created from analysis of the combined RAPD+ISSR data was more similar to the RAPD-only dendrogram than the ISSR-only analysis, indicating that RAPD could determine genetic diversity with higher resolution than ISSR in the cultivars tested. High correlation between the RAPD and ISSR marker systems was shown using a Mantel test (r = 0.92). Screening a higher number of anonymous loci in sugar beet using these molecular markers will enable the selection of the best parent cultivars for the development of novel varieties.

 

Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers were used for comparative analysis of genetic variation in 42 sugar beet accessions. A total of 24 polymorphic primers (12 RAPD and 12 ISSR) were used. The RAPD primers generated 204 amplification products and the ISSR primers produced 178 fragments, 190 and 173 of which were polymorphic, respectively. The average polymorphism level with the ISSR markers (97.2%) was higher than that with the RAPD primers (93%). High genetic diversity indices for both marker types (0.86 for RAPD and 0.91 for ISSR) suggested that these methods were equally effective in determining genetic variation in sugar beet accessions. Cluster analysis of the RAPD, ISSR, and combined datasets revealed similar grouping patterns. However, the dendrogram created from analysis of the combined RAPD+ISSR data was more similar to the RAPD-only dendrogram than the ISSR-only analysis, indicating that RAPD could determine genetic diversity with higher resolution than ISSR in the cultivars tested. High correlation between the RAPD and ISSR marker systems was shown using a Mantel test (r = 0.92). Screening a higher number of anonymous loci in sugar beet using these molecular markers will enable the selection of the best parent cultivars for the development of novel varieties.

Makale detayları

Efficiency of using RAPD and ISSR markers in evaluation of genetic diversity in sugar beet

Yazarlar:

Vusala IZZATULLAYEVA | Zeynal AKPAROV | Sevda BABAYEVA | Javid OJAGHI | Mehraj ABBASOV

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.429-438

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812149

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Sugar beet

genetic diversity

molecular markers

RAPD

ISSR

Sayı

Makale detayları

Efficiency of using RAPD and ISSR markers in evaluation of genetic diversity in sugar beet

Yazarlar: Vusala IZZATULLAYEVA | Zeynal AKPAROV | Sevda BABAYEVA | Javid OJAGHI | Mehraj ABBASOV

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.429-438

Belge Tipi: Makale

Yayın Tarihi: 3/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000812149

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sugar beet

genetic diversity

molecular markers

RAPD

ISSR

Sayı

Yazarlar:

Vusala IZZATULLAYEVA | Zeynal AKPAROV | Sevda BABAYEVA | Javid OJAGHI | Mehraj ABBASOV

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.429-438

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812149

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152