Resveratrol treatment reduces apoptosis and morphological alterations in cisplatin induced testis damage

Nagehan Özyilmaz Yay; Göksel Şener; Feriha Ercan

Makale detayları

Resveratrol treatment reduces apoptosis and morphological alterations in cisplatin induced testis damage

Yazarlar:

Nagehan Özyilmaz Yay | Göksel Şener | Feriha Ercan

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 4, pp.621-631

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347680

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Cisplatin commonly used as a chemotheropotic agent however, it is associated with numerous side effects such as reproductive cytotoxicity. It causes spermatogenic cell death and DNA damage in spermatozoa via the formation of reactive oxygene species. Resveratrol 3,5,4-trans-trihydroxystilbene, a natural phytoalexin, is a potent antioxidant agent, present in a wide variety of dietary sources including grapes, plums and peanuts. The aim of present study to evaluate the beneficial effects of resveratrol on cisplatin induced testis damage. Male Sprague Dawley rats were used in the study and four experimental groups were formed as: 1- saline applied control, 2- resveratrol applied control, 3- cisplatin and 4- cisplatinresveratrol groups. Following a single dose of cisplatin 7 mgkg i.p., either saline or resveratrol 10 mgkg, orally was administered for 5 days. Testis samples were prepared for histopathological and ultrastructural evaluations, cell proliferation and apoptosis. Tissue malondialdehyde MDA, glutathione GSH levels and myeloperoxidase MPO activity were determined biochemically. Degenerated and atrophic tubules of tissue, apoptotic cells, MDA level and MPO activity were increased although proliferation index and GSH level were decreased in cisplatin group. Degenerated tight junctions between the Sertoli cells and vacuole formation in germinal epithelial cells were also revealed at this group. However, resveratrol treatment reduced degenerated and atrophic tubules, apoptotic cells, vacuole formation in germinal epithelial cells, MDA level and MPO activity and increased proliferation index and GSH level in testis. These results showed that resveratrol ameliorates cisplatin induced testis injury by the impairment of oxidative stress and apoptosis.

 Cisplatin commonly used as a chemotheropotic agent however, it is associated with numerous side effects such as reproductive cytotoxicity. It causes spermatogenic cell death and DNA damage in spermatozoa via the formation of reactive oxygene species. Resveratrol 3,5,4-trans-trihydroxystilbene, a natural phytoalexin, is a potent antioxidant agent, present in a wide variety of dietary sources including grapes, plums and peanuts. The aim of present study to evaluate the beneficial effects of resveratrol on cisplatin induced testis damage. Male Sprague Dawley rats were used in the study and four experimental groups were formed as: 1- saline applied control, 2- resveratrol applied control, 3- cisplatin and 4- cisplatinresveratrol groups. Following a single dose of cisplatin 7 mgkg i.p., either saline or resveratrol 10 mgkg, orally was administered for 5 days. Testis samples were prepared for histopathological and ultrastructural evaluations, cell proliferation and apoptosis. Tissue malondialdehyde MDA, glutathione GSH levels and myeloperoxidase MPO activity were determined biochemically. Degenerated and atrophic tubules of tissue, apoptotic cells, MDA level and MPO activity were increased although proliferation index and GSH level were decreased in cisplatin group. Degenerated tight junctions between the Sertoli cells and vacuole formation in germinal epithelial cells were also revealed at this group. However, resveratrol treatment reduced degenerated and atrophic tubules, apoptotic cells, vacuole formation in germinal epithelial cells, MDA level and MPO activity and increased proliferation index and GSH level in testis. These results showed that resveratrol ameliorates cisplatin induced testis injury by the impairment of oxidative stress and apoptosis.

 Cisplatin commonly used as a chemotheropotic agent however, it is associated with numerous side effects such as reproductive cytotoxicity. It causes spermatogenic cell death and DNA damage in spermatozoa via the formation of reactive oxygene species. Resveratrol 3,5,4-trans-trihydroxystilbene, a natural phytoalexin, is a potent antioxidant agent, present in a wide variety of dietary sources including grapes, plums and peanuts. The aim of present study to evaluate the beneficial effects of resveratrol on cisplatin induced testis damage. Male Sprague Dawley rats were used in the study and four experimental groups were formed as: 1- saline applied control, 2- resveratrol applied control, 3- cisplatin and 4- cisplatinresveratrol groups. Following a single dose of cisplatin 7 mgkg i.p., either saline or resveratrol 10 mgkg, orally was administered for 5 days. Testis samples were prepared for histopathological and ultrastructural evaluations, cell proliferation and apoptosis. Tissue malondialdehyde MDA, glutathione GSH levels and myeloperoxidase MPO activity were determined biochemically. Degenerated and atrophic tubules of tissue, apoptotic cells, MDA level and MPO activity were increased although proliferation index and GSH level were decreased in cisplatin group. Degenerated tight junctions between the Sertoli cells and vacuole formation in germinal epithelial cells were also revealed at this group. However, resveratrol treatment reduced degenerated and atrophic tubules, apoptotic cells, vacuole formation in germinal epithelial cells, MDA level and MPO activity and increased proliferation index and GSH level in testis. These results showed that resveratrol ameliorates cisplatin induced testis injury by the impairment of oxidative stress and apoptosis.

 Cisplatin commonly used as a chemotheropotic agent however, it is associated with numerous side effects such as reproductive cytotoxicity. It causes spermatogenic cell death and DNA damage in spermatozoa via the formation of reactive oxygene species. Resveratrol 3,5,4-trans-trihydroxystilbene, a natural phytoalexin, is a potent antioxidant agent, present in a wide variety of dietary sources including grapes, plums and peanuts. The aim of present study to evaluate the beneficial effects of resveratrol on cisplatin induced testis damage. Male Sprague Dawley rats were used in the study and four experimental groups were formed as: 1- saline applied control, 2- resveratrol applied control, 3- cisplatin and 4- cisplatinresveratrol groups. Following a single dose of cisplatin 7 mgkg i.p., either saline or resveratrol 10 mgkg, orally was administered for 5 days. Testis samples were prepared for histopathological and ultrastructural evaluations, cell proliferation and apoptosis. Tissue malondialdehyde MDA, glutathione GSH levels and myeloperoxidase MPO activity were determined biochemically. Degenerated and atrophic tubules of tissue, apoptotic cells, MDA level and MPO activity were increased although proliferation index and GSH level were decreased in cisplatin group. Degenerated tight junctions between the Sertoli cells and vacuole formation in germinal epithelial cells were also revealed at this group. However, resveratrol treatment reduced degenerated and atrophic tubules, apoptotic cells, vacuole formation in germinal epithelial cells, MDA level and MPO activity and increased proliferation index and GSH level in testis. These results showed that resveratrol ameliorates cisplatin induced testis injury by the impairment of oxidative stress and apoptosis.

Makale detayları

Resveratrol treatment reduces apoptosis and morphological alterations in cisplatin induced testis damage

Yazarlar:

Nagehan Özyilmaz Yay | Göksel Şener | Feriha Ercan

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 4, pp.621-631

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347680

pISSN:

2630-6344

Cisplatin

resveratrol

testis

apoptosis

cell proliferation

ultrastructure.

Sayı

Makale detayları

Resveratrol treatment reduces apoptosis and morphological alterations in cisplatin induced testis damage

Yazarlar: Nagehan Özyilmaz Yay | Göksel Şener | Feriha Ercan

Yayıncı: Marmara University

Ana Başlıklar: Basic Pharmacology

Dergi: Journal of Research in Pharmacy Vol.23 Issue 4, pp.621-631

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347680

pISSN: 2630-6344

Cisplatin

resveratrol

testis

apoptosis

cell proliferation

ultrastructure.

Sayı

Yazarlar:

Nagehan Özyilmaz Yay | Göksel Şener | Feriha Ercan

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 4, pp.621-631

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347680

pISSN:

2630-6344