Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Aytül KÜRÜM; Siyami KARAHAN; Hakan KOCAMIŞ; Miyase ÇINAR; Emel ERGÜN

Makale detayları

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Yazarlar:

Aytül KÜRÜM | Siyami KARAHAN | Hakan KOCAMIŞ | Miyase ÇINAR | Emel ERGÜN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2019 Vol.43 Issue 4, pp.448-455

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000764204

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages in both sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases with a significant difference for the estral phase (P < 0.05), and it decreased over the passages with no significant differences. In conclusion, the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higher in the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitro environment.

Makale detayları

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Yazarlar: Aytül KÜRÜM | Siyami KARAHAN | Hakan KOCAMIŞ | Miyase ÇINAR | Emel ERGÜN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2019 Vol.43 Issue 4, pp.448-455

Belge Tipi: Makale

Yayın Tarihi: 7/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000764204

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Aytül KÜRÜM | Siyami KARAHAN | Hakan KOCAMIŞ | Miyase ÇINAR | Emel ERGÜN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2019 Vol.43 Issue 4, pp.448-455

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000764204

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128