Evaluation of the Cytotoxic Effect and Possible Mechanisms of Carnosol and Carnosic Acid in Human Endometrioid Ovarian Adenocarcinoma Cell Line MDAH-2774 Cells

Hazal Demir; Hatice Örs; Ebru Alimoğulları; Aslı Ceylan

Makale detayları

Evaluation of the Cytotoxic Effect and Possible Mechanisms of Carnosol and Carnosic Acid in Human Endometrioid Ovarian Adenocarcinoma Cell Line MDAH-2774 Cells

Yazarlar:

Hazal Demir | Hatice Örs | Ebru Alimoğulları | Aslı Ceylan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 3, pp.322-328

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001334956

pISSN:

2148-9645

Anahtar kelimeler:

Özet:

 Objective: Ovarian cancer is one of the most lethal forms of gynecological malignancies in women and is often detected at an advanced stage. Cytotoxic treatments have been investigated to sensitize cancer cells. Naturally occurring products are becoming an attractive method for suppressing cancer growth because of their cytotoxic properties. Both carnosol and carnosic acid are diterpenoids with antioxidant, anti- inflammatory, anti-proliferative, and anti-cancer activities. The present study aims to investigate the effects of both carnosol and carnosic acid on the MDAH-2774 cell line. Material and Methods: Different concentrations and time intervals were used in the present study. We employed MTT to show cell survival in the presence and absence of carnosol andor carnosic acid. To understand the mechanisms behind the effects of the agents on cell survival, total antioxidant capacity was evaluated. Results: Our results showed that the agents, alone and more so in combination, successfully reduced the viability of MDAH- 2774 cells. Total antioxidant capacity analysis revealed that the compounds had high antioxidant capacities both when used alone and when combined. Conclusion: Our study determined the cytotoxic effects of carnosol and carnosic acid, both when used alone and in combination. These effects may be attributed to their antioxidant properties, with the combined form exhibiting a more pronounced impact.

 Objective: Ovarian cancer is one of the most lethal forms of gynecological malignancies in women and is often detected at an advanced stage. Cytotoxic treatments have been investigated to sensitize cancer cells. Naturally occurring products are becoming an attractive method for suppressing cancer growth because of their cytotoxic properties. Both carnosol and carnosic acid are diterpenoids with antioxidant, anti- inflammatory, anti-proliferative, and anti-cancer activities. The present study aims to investigate the effects of both carnosol and carnosic acid on the MDAH-2774 cell line. Material and Methods: Different concentrations and time intervals were used in the present study. We employed MTT to show cell survival in the presence and absence of carnosol andor carnosic acid. To understand the mechanisms behind the effects of the agents on cell survival, total antioxidant capacity was evaluated. Results: Our results showed that the agents, alone and more so in combination, successfully reduced the viability of MDAH- 2774 cells. Total antioxidant capacity analysis revealed that the compounds had high antioxidant capacities both when used alone and when combined. Conclusion: Our study determined the cytotoxic effects of carnosol and carnosic acid, both when used alone and in combination. These effects may be attributed to their antioxidant properties, with the combined form exhibiting a more pronounced impact.

 Objective: Ovarian cancer is one of the most lethal forms of gynecological malignancies in women and is often detected at an advanced stage. Cytotoxic treatments have been investigated to sensitize cancer cells. Naturally occurring products are becoming an attractive method for suppressing cancer growth because of their cytotoxic properties. Both carnosol and carnosic acid are diterpenoids with antioxidant, anti- inflammatory, anti-proliferative, and anti-cancer activities. The present study aims to investigate the effects of both carnosol and carnosic acid on the MDAH-2774 cell line. Material and Methods: Different concentrations and time intervals were used in the present study. We employed MTT to show cell survival in the presence and absence of carnosol andor carnosic acid. To understand the mechanisms behind the effects of the agents on cell survival, total antioxidant capacity was evaluated. Results: Our results showed that the agents, alone and more so in combination, successfully reduced the viability of MDAH- 2774 cells. Total antioxidant capacity analysis revealed that the compounds had high antioxidant capacities both when used alone and when combined. Conclusion: Our study determined the cytotoxic effects of carnosol and carnosic acid, both when used alone and in combination. These effects may be attributed to their antioxidant properties, with the combined form exhibiting a more pronounced impact.

 Amaç: Over kanseri, kadınlarda en yüksek ölüm oranına sahip olan ve genellikle ileri evrede teşhis edilebilen bir jinekolojik kanser türüdür. Kanser hücrelerini duyarlı hale getirmek için sitotoksik tedaviler araştırılmaktadır. Sitotoksik etkileri olduğu bilinen bitki kaynaklı ürünlerin kullanımı, kanser büyümesini baskılamada ilgi çekici bir yöntem haline gelmiştir. Hem karnosol hem de karnosik asit, antioksidan, anti-inflamatuvar, anti-proliferatif ve antikanser aktivitelere sahip olan bitkisel diterpenoidlerdir. Yaptığımız bu çalışmanın amacı hem karnosol hem de karnosik asidin MDAH-2774 over endometrioid kanseri hücre hattı üzerindeki etkilerini araştırmaktır. Gereç ve Yöntemler: Çalışmamızda farklı konsantrasyonlar ve zaman aralıkları kullanılmıştır. Karnosol veveya karnosik asidin varlığında ve yokluğunda hücre canlılığı üzerine olan etkiler, MTT analizi ile saptanmıştır. Ajanların hücre canlılığı üzerindeki etkilerinin ardındaki mekanizmayı anlamak için hücrelerin toplam antioksidan durumu değerlendirilmiştir. Bulgular: Sonuçlarımız, ajanların tek başlarına ve daha çok da kombine halde MDAH-2774 hücre canlılığını başarıyla azalttığını göstermiştir. Toplam antioksidan durum analizi bileşiklerin ayrı ayrı ve kombine halde antioksidan kapasitelerinin yüksekliğini göstermiştir. Sonuç: Çalışmamız, karnosol ve karnosik asidin tek başına ve kombine halde gösterdikleri sitotoksik etkinin onların antioksidan etkileriyle ilişkili olabileceğini ve bu etkinin kombine halde daha güçlü olduğunu saptamıştır.

Makale detayları

Evaluation of the Cytotoxic Effect and Possible Mechanisms of Carnosol and Carnosic Acid in Human Endometrioid Ovarian Adenocarcinoma Cell Line MDAH-2774 Cells

Yazarlar:

Hazal Demir | Hatice Örs | Ebru Alimoğulları | Aslı Ceylan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 3, pp.322-328

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001334956

pISSN:

2148-9645

Carnosol

carnosic acid

MDAH-2774

ovarian cancer

cytotoxic effect

total antioxidant statusKarnosol

karnosik asit

MDAH-2774

over kanseri

sitotoksik etki

total antioksidan durum

Sayı

Makale detayları

Yazarlar: Hazal Demir | Hatice Örs | Ebru Alimoğulları | Aslı Ceylan

Yayıncı: Kirikkale University

Ana Başlıklar: Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 3, pp.322-328

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001334956

pISSN: 2148-9645

Sayı

Yazarlar:

Hazal Demir | Hatice Örs | Ebru Alimoğulları | Aslı Ceylan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi Vol.26 Issue 3, pp.322-328

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001334956

pISSN:

2148-9645