Determination of Antimicrobial, Antibiofilm, Antioxidant Activity and Phenolic Profile of Alyssum filiforme Plant

İlayda Orçan; Ali Savaş Bülbül

Makale detayları

Determination of Antimicrobial, Antibiofilm, Antioxidant Activity and Phenolic Profile of Alyssum filiforme Plant

Yazarlar:

İlayda Orçan | Ali Savaş Bülbül

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Structural Biology

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.9 Issue 1, pp.48-55

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284616

pISSN:

2757-5195

Anahtar kelimeler:

Özet:

 Bacteria are gaining resistance to antibiotics and their derivatives day by day, and this is becoming a global problem. It is thought that this global problem can be prevented due to the antioxidant and antimicrobial activities of the secondary metabolites phenolic compounds, etc. found in plants. In this context, various studies have been carried out. Resistance to antibiotics and their derivatives can be overcome by identifying components that act as antioxidants, antimicrobials, and anti-biofilms from plant sources. Alyssum filiforme is a member of the Brassicacea family, which is rich in B vitamins and magnesium. Alyssum filiforme is an endemic species with the potential to be evaluated in this context. In this study, the antimicrobial, anti-biofilm, antioxidant, and phenolic profile of the endemic Alyssum filiforme species was determined by RP-HPLC-PDA. Minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC methods were applied for the antimicrobial activity of the obtained extract, and 2,2-diphenyl-1-picrylhydrazil DPPH radical scavenging activity and iron reducing antioxidant power FRAP were used to determine the antioxidant activity. To observe the effectiveness in preventing biofilm formation, the crystal violet bonding method was used, and positive results were obtained in all bacteria. FRAP and DPPH results were determined as 13.070 μmol FeSO4.7H2Og and SC50 5.190 mgml, respectively. As a result of the obtained phenolic analysis, it was found that the plant is rich in caffeic acid and chrysin. In the light of the results obtained, Alyssum filiforme is thought to be a valuable species in terms of health.

 Bacteria are gaining resistance to antibiotics and their derivatives day by day, and this is becoming a global problem. It is thought that this global problem can be prevented due to the antioxidant and antimicrobial activities of the secondary metabolites phenolic compounds, etc. found in plants. In this context, various studies have been carried out. Resistance to antibiotics and their derivatives can be overcome by identifying components that act as antioxidants, antimicrobials, and anti-biofilms from plant sources. Alyssum filiforme is a member of the Brassicacea family, which is rich in B vitamins and magnesium. Alyssum filiforme is an endemic species with the potential to be evaluated in this context. In this study, the antimicrobial, anti-biofilm, antioxidant, and phenolic profile of the endemic Alyssum filiforme species was determined by RP-HPLC-PDA. Minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC methods were applied for the antimicrobial activity of the obtained extract, and 2,2-diphenyl-1-picrylhydrazil DPPH radical scavenging activity and iron reducing antioxidant power FRAP were used to determine the antioxidant activity. To observe the effectiveness in preventing biofilm formation, the crystal violet bonding method was used, and positive results were obtained in all bacteria. FRAP and DPPH results were determined as 13.070 μmol FeSO4.7H2Og and SC50 5.190 mgml, respectively. As a result of the obtained phenolic analysis, it was found that the plant is rich in caffeic acid and chrysin. In the light of the results obtained, Alyssum filiforme is thought to be a valuable species in terms of health.

 Bacteria are gaining resistance to antibiotics and their derivatives day by day, and this is becoming a global problem. It is thought that this global problem can be prevented due to the antioxidant and antimicrobial activities of the secondary metabolites phenolic compounds, etc. found in plants. In this context, various studies have been carried out. Resistance to antibiotics and their derivatives can be overcome by identifying components that act as antioxidants, antimicrobials, and anti-biofilms from plant sources. Alyssum filiforme is a member of the Brassicacea family, which is rich in B vitamins and magnesium. Alyssum filiforme is an endemic species with the potential to be evaluated in this context. In this study, the antimicrobial, anti-biofilm, antioxidant, and phenolic profile of the endemic Alyssum filiforme species was determined by RP-HPLC-PDA. Minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC methods were applied for the antimicrobial activity of the obtained extract, and 2,2-diphenyl-1-picrylhydrazil DPPH radical scavenging activity and iron reducing antioxidant power FRAP were used to determine the antioxidant activity. To observe the effectiveness in preventing biofilm formation, the crystal violet bonding method was used, and positive results were obtained in all bacteria. FRAP and DPPH results were determined as 13.070 μmol FeSO4.7H2Og and SC50 5.190 mgml, respectively. As a result of the obtained phenolic analysis, it was found that the plant is rich in caffeic acid and chrysin. In the light of the results obtained, Alyssum filiforme is thought to be a valuable species in terms of health.

 Bakteriler antibiyotiklere ve türevlerine karşı günden güne direnç kazanmaktadır ve bu küresel bir sorun haline gelmektedir. Bitkilerde bulunan sekonder metabolitlerin fenolik bileşikler, flavonoidler, alkoloidler gibi antioksidan ve antimikrobiyal gibi çeşitli aktiviteleri sebebiyle bu küresel sorunun önüne geçilebileceği düşünülmektedir. Bu kapsamda çeşitli çalışmalar yapılmış ve halen de yapılmaktadır. Bitkisel kaynaklardan antioksidan, antimikrobiyal ve anti-biyofilm gibi etki gösteren bileşenlerin belirlenmesi ile antibiyotik ve türevlerine karşı oluşan direnç aşılabilir. B vitamini ve magnezyum açısından zengin olan Brassicacea ailesinin bir üyesi de Alyssum filiforme türüdür. Alyssum filiforme bu kapsamda değerlendirilebilecek potansiyele sahip bir endemik türdür. Bu çalışmada; endemik olarak yetişen türün antimikrobiyal, anti-biyofilm, antioksidan ve RP-HPLC-PDA ile fenolik profili belirlendi. Elde edilen ekstre antimikrobiyal aktivite için Minimum İnhibisyon Konsantrasyonu MIC ve Minimum Bakterisidal Konsantrasyonu MBC yöntemleri uygulandı, antioksidan aktiviteyi belirlemek için ise 2,2-difenil-1-pikrilhidrazil DPPH radikalini temizleme aktivitesi ve demir indirgeme antioksidan güç FRAP metodları kullanıldı. Biyofilm oluşumunu önlemedeki etkinliği gözlemleyebilmek için kristal viyole bağlama yöntemi kullanıldı. Tüm bakterilerde olumlu sonuçlar elde edildi. FRAP ve DPPH sonuçları sırasıyla 13.070 μmol FeSO4.7H2Og ve SC50 5.190 mgml olarak belirlendi. Elde edilen fenolik analiz sonucunda bitkinin kafeik asit ve krisin yönünden zengin olduğu bulundu. Elde edilen sonuçlar ışığında Alyssum filiforme türünün sağlık açısından değerli bir tür olduğu düşünülmektedir.

Makale detayları

Determination of Antimicrobial, Antibiofilm, Antioxidant Activity and Phenolic Profile of Alyssum filiforme Plant

Yazarlar:

İlayda Orçan | Ali Savaş Bülbül

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Structural Biology

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.9 Issue 1, pp.48-55

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284616

pISSN:

2757-5195

antioxidant

phenolic compound

Alyssum filiforme

antibiyofilm

antibacterial

Alyssum filiforme

antibiyofilm

antioksidan

fenolik bileşikler

antibakteriyel

Sayı

Makale detayları

Yazarlar: İlayda Orçan | Ali Savaş Bülbül

Yayıncı: Çanakkale Onsekiz Mart University

Ana Başlıklar: Structural Biology

Dergi: Journal of Advanced Research in Natural and Applied Sciences Vol.9 Issue 1, pp.48-55

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001284616

pISSN: 2757-5195

Sayı

Yazarlar:

İlayda Orçan | Ali Savaş Bülbül

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Structural Biology

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.9 Issue 1, pp.48-55

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284616

pISSN:

2757-5195