The Role of Steroid Receptors in the Proliferation and Migration of Endometrial Adenocarcinoma HEC1A Cell Line

R. Nalan Tiftik; İsmail Ün; Kansu Büyükafşar

Makale detayları

The Role of Steroid Receptors in the Proliferation and Migration of Endometrial Adenocarcinoma HEC1A Cell Line

Yazarlar:

R. Nalan Tiftik | İsmail Ün | Kansu Büyükafşar

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi

Dergi:

Dicle Tıp Dergisi , pp.327-334

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298564

pISSN:

1300-2945

Anahtar kelimeler:

Özet:

 Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and oestrogen-independent type II. Although the expression of some steroid receptors has been documented in type II endometrial carcinoma, their roles in tumor progression have not been fully elucidated yet. Thus in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells. Methods: We tested the effect of mifepristone the glucocorticoid and progesterone receptor blocker, 10-8M, bicalutamide the androgen receptor blocker, 10-6M, G15 the G-protein-coupled estrogen receptor-1 blocker, 10-7M and PHTPP 2-Phenyl-3-4-hydroxyphenyl-5,7-bis trifluoromethyl-pyrazolo 1,5-apyrimidine, the estrogen receptor-β blocker, 10-7M, on proliferation. Proliferation was assessed by xCELLigence analysis system and migration was examined by using wound-healing model. Results: None of the drugs, at the used concentrations, have affected the proliferation of HEC1A cells. However, migration was significantly increased at the 24th and the 48th hour of mifepristone application p0.05, P0.01, respectively. Bicalutamide also increased migration at the 24th hour p0.05. G15 and PHTPP did not change the migration of cells. Conclusions: These results suggest that, unlike other steroid receptors, glucocorticoid andor progesterone receptors may play an important role in the reducing migration of endometrial carcinoma and might be used as targets to reduce the metastasis of this type of cancer.

 Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and oestrogen-independent type II. Although the expression of some steroid receptors has been documented in type II endometrial carcinoma, their roles in tumor progression have not been fully elucidated yet. Thus in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells. Methods: We tested the effect of mifepristone the glucocorticoid and progesterone receptor blocker, 10-8M, bicalutamide the androgen receptor blocker, 10-6M, G15 the G-protein-coupled estrogen receptor-1 blocker, 10-7M and PHTPP 2-Phenyl-3-4-hydroxyphenyl-5,7-bis trifluoromethyl-pyrazolo 1,5-apyrimidine, the estrogen receptor-β blocker, 10-7M, on proliferation. Proliferation was assessed by xCELLigence analysis system and migration was examined by using wound-healing model. Results: None of the drugs, at the used concentrations, have affected the proliferation of HEC1A cells. However, migration was significantly increased at the 24th and the 48th hour of mifepristone application p0.05, P0.01, respectively. Bicalutamide also increased migration at the 24th hour p0.05. G15 and PHTPP did not change the migration of cells. Conclusions: These results suggest that, unlike other steroid receptors, glucocorticoid andor progesterone receptors may play an important role in the reducing migration of endometrial carcinoma and might be used as targets to reduce the metastasis of this type of cancer.

 Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and oestrogen-independent type II. Although the expression of some steroid receptors has been documented in type II endometrial carcinoma, their roles in tumor progression have not been fully elucidated yet. Thus in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells. Methods: We tested the effect of mifepristone the glucocorticoid and progesterone receptor blocker, 10-8M, bicalutamide the androgen receptor blocker, 10-6M, G15 the G-protein-coupled estrogen receptor-1 blocker, 10-7M and PHTPP 2-Phenyl-3-4-hydroxyphenyl-5,7-bis trifluoromethyl-pyrazolo 1,5-apyrimidine, the estrogen receptor-β blocker, 10-7M, on proliferation. Proliferation was assessed by xCELLigence analysis system and migration was examined by using wound-healing model. Results: None of the drugs, at the used concentrations, have affected the proliferation of HEC1A cells. However, migration was significantly increased at the 24th and the 48th hour of mifepristone application p0.05, P0.01, respectively. Bicalutamide also increased migration at the 24th hour p0.05. G15 and PHTPP did not change the migration of cells. Conclusions: These results suggest that, unlike other steroid receptors, glucocorticoid andor progesterone receptors may play an important role in the reducing migration of endometrial carcinoma and might be used as targets to reduce the metastasis of this type of cancer.

 Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and oestrogen-independent type II. Although the expression of some steroid receptors has been documented in type II endometrial carcinoma, their roles in tumor progression have not been fully elucidated yet. Thus in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells. Methods: We tested the effect of mifepristone the glucocorticoid and progesterone receptor blocker, 10-8M, bicalutamide the androgen receptor blocker, 10-6M, G15 the G-protein-coupled estrogen receptor-1 blocker, 10-7M and PHTPP 2-Phenyl-3-4-hydroxyphenyl-5,7-bis trifluoromethyl-pyrazolo 1,5-apyrimidine, the estrogen receptor-β blocker, 10-7M, on proliferation. Proliferation was assessed by xCELLigence analysis system and migration was examined by using wound-healing model. Results: None of the drugs, at the used concentrations, have affected the proliferation of HEC1A cells. However, migration was significantly increased at the 24th and the 48th hour of mifepristone application p0.05, P0.01, respectively. Bicalutamide also increased migration at the 24th hour p0.05. G15 and PHTPP did not change the migration of cells. Conclusions: These results suggest that, unlike other steroid receptors, glucocorticoid andor progesterone receptors may play an important role in the reducing migration of endometrial carcinoma and might be used as targets to reduce the metastasis of this type of cancer.

Makale detayları

The Role of Steroid Receptors in the Proliferation and Migration of Endometrial Adenocarcinoma HEC1A Cell Line

Yazarlar:

R. Nalan Tiftik | İsmail Ün | Kansu Büyükafşar

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi

Dergi:

Dicle Tıp Dergisi , pp.327-334

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298564

pISSN:

1300-2945

Endometrial adenocarcinoma

steroid receptors

proliferation; migration

Sayı

Makale detayları

The Role of Steroid Receptors in the Proliferation and Migration of Endometrial Adenocarcinoma HEC1A Cell Line

Yazarlar: R. Nalan Tiftik | İsmail Ün | Kansu Büyükafşar

Yayıncı: Dicle University

Ana Başlıklar: Tıp Eğitimi

Dergi: Dicle Tıp Dergisi , pp.327-334

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001298564

pISSN: 1300-2945

Endometrial adenocarcinoma

steroid receptors

proliferation; migration

Sayı

Yazarlar:

R. Nalan Tiftik | İsmail Ün | Kansu Büyükafşar

Yayıncı:

Dicle University

Ana Başlıklar:

Tıp Eğitimi

Dergi:

Dicle Tıp Dergisi , pp.327-334

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298564

pISSN:

1300-2945