The Effects of Sodium Selenite on the Antioxidative Defence Mechanism of Human Hepatoma G2 Cells

Kayahan FIŞKIN

Makale detayları

The Effects of Sodium Selenite on the Antioxidative Defence Mechanism of Human Hepatoma G2 Cells

Yazarlar:

Kayahan FIŞKIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.203-208

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757598

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Human cells that live in biological  harmony can become cancerous. As they do  so, they engage in anaerobic respiration,  undergo rapid cell growth and division  according to nonmitotic rules, and important  changes take place in their metabolism. We  conducted several investigations in an effort  to block or change the metabolic  characteristics of cancer cells using a typical  cancerous cell line. Specifically,we  investigated the effects of sodium selenite  (Na2 SeO 3 ) on antioxidant enzyme activities  and reduced glutathione levels in Hepatoma  G 2 cells, cells that show rapid glutamine  uptake and metabolism.  A Hepatoma G 2 cell line was cultured in RPMI  1640 Dutch medium. Sodium selenite  solution was added to the culture medium to  produce a final concentration of 1µM/ml on  the second day of incubation and several  measurements of metabolic activity were  taken.The selenium treatment increased  selenium-dependent glutathione peroxidase  and Cu,Zn super oxide dismutase activities by  approximately 100% and 21% respectively,  yet reduced catalase activity by 20%. There  was no Mn-super oxide dismutase activity in  the Hepatoma G2 cells, but the existence of  an inactive form of Mn-super oxide dismutase  was observed. Glutathione S-transferase  activity was not affected by selenium.  Selenium caused a 56% decrease in reduced  glutathione level in a Hepatoma G2 cell line.  We argue that the observed changes are due  to selenium dependent protein as a Se-GSH-Px.  The unchanging activity levels of  glutathione-S transferase is important  because it plays a key role in cellular  detoxification mechanisms. We conclude that  glutathione metabolism in cancer cells slows  with selenium treatment.

 

Human cells that live in biological  harmony can become cancerous. As they do  so, they engage in anaerobic respiration,  undergo rapid cell growth and division  according to nonmitotic rules, and important  changes take place in their metabolism. We  conducted several investigations in an effort  to block or change the metabolic  characteristics of cancer cells using a typical  cancerous cell line. Specifically,we  investigated the effects of sodium selenite  (Na2 SeO 3 ) on antioxidant enzyme activities  and reduced glutathione levels in Hepatoma  G 2 cells, cells that show rapid glutamine  uptake and metabolism.  A Hepatoma G 2 cell line was cultured in RPMI  1640 Dutch medium. Sodium selenite  solution was added to the culture medium to  produce a final concentration of 1µM/ml on  the second day of incubation and several  measurements of metabolic activity were  taken.The selenium treatment increased  selenium-dependent glutathione peroxidase  and Cu,Zn super oxide dismutase activities by  approximately 100% and 21% respectively,  yet reduced catalase activity by 20%. There  was no Mn-super oxide dismutase activity in  the Hepatoma G2 cells, but the existence of  an inactive form of Mn-super oxide dismutase  was observed. Glutathione S-transferase  activity was not affected by selenium.  Selenium caused a 56% decrease in reduced  glutathione level in a Hepatoma G2 cell line.  We argue that the observed changes are due  to selenium dependent protein as a Se-GSH-Px.  The unchanging activity levels of  glutathione-S transferase is important  because it plays a key role in cellular  detoxification mechanisms. We conclude that  glutathione metabolism in cancer cells slows  with selenium treatment.

Makale detayları

The Effects of Sodium Selenite on the Antioxidative Defence Mechanism of Human Hepatoma G2 Cells

Yazarlar:

Kayahan FIŞKIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.203-208

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757598

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Hepatoma G2

Sodium selenite

Glutathione peroxidase

Catalase

Superoxide dismutase.

Sayı

Makale detayları

The Effects of Sodium Selenite on the Antioxidative Defence Mechanism of Human Hepatoma G2 Cells

Yazarlar: Kayahan FIŞKIN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.203-208

Belge Tipi: Makale

Yayın Tarihi: 2/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000757598

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Hepatoma G2

Sodium selenite

Glutathione peroxidase

Catalase

Superoxide dismutase.

Sayı

Yazarlar:

Kayahan FIŞKIN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 3, pp.203-208

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757598

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144