Investigation of the Effects of Bilberry Extract on Methotrexate-Induced  Hepatotoxicity

Gökçen Kerimoğlu; Gamze Bilgin; Servet Kerimoğlu; Nihal Türkmen Alemdar; Selim Demir; Yüksel Aliyazıcıoğlu

Makale detayları

Investigation of the Effects of Bilberry Extract on Methotrexate-Induced Hepatotoxicity

Yazarlar:

Gökçen Kerimoğlu | Gamze Bilgin | Servet Kerimoğlu | Nihal Türkmen Alemdar | Selim Demir | Yüksel Aliyazıcıoğlu

Yayıncı:

Harran University

Ana Başlıklar:

Gastroenteroloji ve Hepatoloji

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.519-524

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001298515

pISSN:

1304-9623

Anahtar kelimeler:

Özet:

 Background: Methotrexate Mtx is widely used in the treatment of cancer and autoimmune diseases. But it can cause toxicity in the liver, which is responsible for the metabolism of many chemotherapeutics such as Mtx. Bilberry Bb fruit has antioxidant and anti-inflammatory effects thanks to the phenolic compounds it contains. This study aims to investigate the effects of oral Bb extract on Mtx-induced hepatotoxicity. Materials and Methods: For the study, 21 Spraque Dawley adult rats were divided into three groups equal in number. One group was given a single dose of 30 mgkg intraperitoneal ip. Mtx on the first day of the experi-ment MtxG. The other group was given a single dose of 30 mgkg ip. Mtx and 200 mgkg Bb extract by oral gavage once a day for five days MtxBbG. No procedure was applied to the control group CG. While some of the liver tissues taken at the end of the experiment were evaluated and scored histologically, the other part was evaluated biochemically. Results: Malondialdehyde MDA and caspase 3 levels were higher in liver tissues of MtxG than CG. Although it is not significant according to CG, there was an increase in 8-hydroxy-2-deoxyguanosine 8-OHdG and oxidative stress index OSI, but a decrease in superoxide dismutase SOD, catalase CAT and total antioxidant capacity TOS in MtxG. In MtxBbG, MDA levels decreased compared to MtxG. In this group, a nonsignificant statististical decrease was also observed in caspase 3, OSI and 8-OHdG levels compared to MtxG. In addition, while the SOD level in this group was lower than in CG; CAT and total antioxidant status TAS levels were higher than in MtxG, although not statistically significant. In MtxG, there was an increase in total damage score, vacuoliza-tiondegenerationpyknosis in hepatocytes, Kupffer cells and sinusoidal vasodilationcongestion compared to the control. In MtxBbG, there was a decrease in all these histopathological findings and the total damage score. Conclusions: Orally administered Bb extract may reduce Mtx-induced oxidative stress, apoptosis, and the hepato-toxicity caused by them in rat liver.

 Background: Methotrexate Mtx is widely used in the treatment of cancer and autoimmune diseases. But it can cause toxicity in the liver, which is responsible for the metabolism of many chemotherapeutics such as Mtx. Bilberry Bb fruit has antioxidant and anti-inflammatory effects thanks to the phenolic compounds it contains. This study aims to investigate the effects of oral Bb extract on Mtx-induced hepatotoxicity. Materials and Methods: For the study, 21 Spraque Dawley adult rats were divided into three groups equal in number. One group was given a single dose of 30 mgkg intraperitoneal ip. Mtx on the first day of the experi-ment MtxG. The other group was given a single dose of 30 mgkg ip. Mtx and 200 mgkg Bb extract by oral gavage once a day for five days MtxBbG. No procedure was applied to the control group CG. While some of the liver tissues taken at the end of the experiment were evaluated and scored histologically, the other part was evaluated biochemically. Results: Malondialdehyde MDA and caspase 3 levels were higher in liver tissues of MtxG than CG. Although it is not significant according to CG, there was an increase in 8-hydroxy-2-deoxyguanosine 8-OHdG and oxidative stress index OSI, but a decrease in superoxide dismutase SOD, catalase CAT and total antioxidant capacity TOS in MtxG. In MtxBbG, MDA levels decreased compared to MtxG. In this group, a nonsignificant statististical decrease was also observed in caspase 3, OSI and 8-OHdG levels compared to MtxG. In addition, while the SOD level in this group was lower than in CG; CAT and total antioxidant status TAS levels were higher than in MtxG, although not statistically significant. In MtxG, there was an increase in total damage score, vacuoliza-tiondegenerationpyknosis in hepatocytes, Kupffer cells and sinusoidal vasodilationcongestion compared to the control. In MtxBbG, there was a decrease in all these histopathological findings and the total damage score. Conclusions: Orally administered Bb extract may reduce Mtx-induced oxidative stress, apoptosis, and the hepato-toxicity caused by them in rat liver.

 Background: Methotrexate Mtx is widely used in the treatment of cancer and autoimmune diseases. But it can cause toxicity in the liver, which is responsible for the metabolism of many chemotherapeutics such as Mtx. Bilberry Bb fruit has antioxidant and anti-inflammatory effects thanks to the phenolic compounds it contains. This study aims to investigate the effects of oral Bb extract on Mtx-induced hepatotoxicity. Materials and Methods: For the study, 21 Spraque Dawley adult rats were divided into three groups equal in number. One group was given a single dose of 30 mgkg intraperitoneal ip. Mtx on the first day of the experi-ment MtxG. The other group was given a single dose of 30 mgkg ip. Mtx and 200 mgkg Bb extract by oral gavage once a day for five days MtxBbG. No procedure was applied to the control group CG. While some of the liver tissues taken at the end of the experiment were evaluated and scored histologically, the other part was evaluated biochemically. Results: Malondialdehyde MDA and caspase 3 levels were higher in liver tissues of MtxG than CG. Although it is not significant according to CG, there was an increase in 8-hydroxy-2-deoxyguanosine 8-OHdG and oxidative stress index OSI, but a decrease in superoxide dismutase SOD, catalase CAT and total antioxidant capacity TOS in MtxG. In MtxBbG, MDA levels decreased compared to MtxG. In this group, a nonsignificant statististical decrease was also observed in caspase 3, OSI and 8-OHdG levels compared to MtxG. In addition, while the SOD level in this group was lower than in CG; CAT and total antioxidant status TAS levels were higher than in MtxG, although not statistically significant. In MtxG, there was an increase in total damage score, vacuoliza-tiondegenerationpyknosis in hepatocytes, Kupffer cells and sinusoidal vasodilationcongestion compared to the control. In MtxBbG, there was a decrease in all these histopathological findings and the total damage score. Conclusions: Orally administered Bb extract may reduce Mtx-induced oxidative stress, apoptosis, and the hepato-toxicity caused by them in rat liver.

 Amaç: Metotreksat Mtx, kanser ve otoimmün hastalıkların tedavisinde yaygın olarak kullanılmaktadır. Ancak Mtx, kendisi gibi birçok kemoterapötiğin metabolizmasından sorumlu olan karaciğerde toksisiteye neden olabilir. Yaban mersini Ym meyvesi, yapısında yoğun olarak bulunan fenolik bileşikler sayesinde antioksidan ve antienflamatuvar etkiye sahiptir. Bu çalışma, Ym ekstresinin, Mtx kaynaklı hepatotoksisiteye olan etkilerini araştırmayı amaçlamakta-dır. Materyal ve metod: Çalışma için 21 adet Spraque Dawley ırkı erişkin sıçan, sayıca eşit üç gruba bölündü. Bir gruba, deneyin ilk günü tek doz 30 mgkg intraperitoneal ip. Mtx verildi MtxG. Diğer gruba tek doz 30 mgkg ip. Mtx ve beş gün boyunca günde bir kez oral gavajla 200 mgkg Ym ekstresi verildi MtxYmG. Kontrol grubuna ise herhangi bir işlem yapılmadı KG. Deney sonunda alınan karaciğer dokularının bir kısmı histolojik olarak değer-lendirilerek skorlanırken diğer kısmı da biyokimyasal olarak değerlendirildi. Bulgular: MtxGnin karaciğer dokularında malondialdehid MDA ve kaspaz 3 düzeyleri KGye göre yüksekti. KGye göre anlamlı olmamakla birlikte MtxGde, 8-hidroksi-2-deoksiguanozin 8-OHdG ve oksidatif stres indeksi OSİnde artış; süperoksit dismutaz SOD, katalaz CAT ve total antioksidan kapasite TOSde ise azalma mevcut-tu. MtxYmGde MDA düzeyleri MtxGye göre azaldı. Bu grupta, kaspaz 3, OSI ve 8-OHdG düzeylerinde de MtxGye göre istatistiksel olarak anlamlı olmayan belirgin bir azalma görüldü. Ayrıca bu gruptaki SOD seviyesi KGye göre düşükken CAT ve total antioksidan kapasite TAS seviyeleri ise MtxGye göre istatistiksel anlamlı olmasa da yüksekti. MtxGde; total hasar skorunda, hepatositlerdeki vakuolizasyondejenerasyonpiknoziste, Kupffer hücrelerinde ve vazodilatasyonkonjesyonda kontrole göre artış mevcuttu. MtxYmGde ise tüm bu histopatolojik bulgularda ve total hasar skorunda azalma mevcuttu. Sonuç: Oral yolla uygulanan Ym ekstresi, sıçan karaciğerindeki Mtx kaynaklı oksidatif stresi, apoptozu ve bunların neden olduğu hepatotoksisiteyi azaltabilir.

Makale detayları

Investigation of the Effects of Bilberry Extract on Methotrexate-Induced Hepatotoxicity

Yazarlar:

Gökçen Kerimoğlu | Gamze Bilgin | Servet Kerimoğlu | Nihal Türkmen Alemdar | Selim Demir | Yüksel Aliyazıcıoğlu

Yayıncı:

Harran University

Ana Başlıklar:

Gastroenteroloji ve Hepatoloji

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.519-524

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001298515

pISSN:

1304-9623

Liver

methotrexate

oxidative stress

bilberryKaraciğer

metotreksat

oksidatif stress

yaban mersini

Sayı

Makale detayları

Yazarlar: Gökçen Kerimoğlu | Gamze Bilgin | Servet Kerimoğlu | Nihal Türkmen Alemdar | Selim Demir | Yüksel Aliyazıcıoğlu

Yayıncı: Harran University

Ana Başlıklar: Gastroenteroloji ve Hepatoloji

Dergi: Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.519-524

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001298515

pISSN: 1304-9623

Sayı

Yazarlar:

Gökçen Kerimoğlu | Gamze Bilgin | Servet Kerimoğlu | Nihal Türkmen Alemdar | Selim Demir | Yüksel Aliyazıcıoğlu

Yayıncı:

Harran University

Ana Başlıklar:

Gastroenteroloji ve Hepatoloji

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.20 Issue 3, pp.519-524

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001298515

pISSN:

1304-9623