Beta Glucosidase Recognition By Imprinted Polyacrylamide Hydrogels

Mervecan SEVİLDİK; Ayşe DİNÇER; Tülin AYDEMİR

Makale detayları

Beta Glucosidase Recognition By Imprinted Polyacrylamide Hydrogels

Yazarlar:

Mervecan SEVİLDİK | Ayşe DİNÇER | Tülin AYDEMİR

Yayıncı:

Muhammet DOĞAN

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Eurasian Journal of Biological and Chemical Sciences 2019 Vol.2 Issue 1, pp.17-23

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000421961

eISSN:

2651-5237

Özet:

 

In the present work, selective adsorption of
β-glucosidase using imprinted polyacrylamide hydrogels were studied. For this
purpose imprinted hydrogels were prepared using β-glucosidase as a template
molecule, acrylamide (AAm) as a monomer, N,N’- methylenebisacrylamide (MBAA) as
a crosslinker, ammonium persulphate (APS) and N,N,N’,N’-tetramethylethylene-diamine
(TEMED) as initiators. β-Glucosidase imprinted hydrogel was washed with a
solution of sodium dodecyl sulfate (SDS) and acetic acid to remove the template
molecule. Non-imprinted hydrogel was also prepared without using β-glucosidase
template. The adsorption and recognition performance of hydrogels towards
β-glucosidase was
discussed through adsorption isotherms and adsorption kinetics. In batch
template rebinding experiments, imprinted hydrogels displayed quite high
template binding capacity than non-imprinted hydrogels. The theoretical maximum
adsorption capacity (Qmax) was determined by the Langmuir model, which turned
out to be 7.2 mg/g  and 4.6 mg/g  for imprinted and non-imprinted
hydrogels respectively. A pseudo-second-order model was suitable to interpret
kinetic data.  .

Makale detayları

Beta Glucosidase Recognition By Imprinted Polyacrylamide Hydrogels

Yazarlar: Mervecan SEVİLDİK | Ayşe DİNÇER | Tülin AYDEMİR

Yayıncı: Muhammet DOĞAN

Ana Başlıklar: Kimya mühendisliği

Dergi: Eurasian Journal of Biological and Chemical Sciences 2019 Vol.2 Issue 1, pp.17-23

Belge Tipi: Makale

Yayın Tarihi: 7/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000421961

eISSN: 2651-5237

Sayı

Yazarlar:

Mervecan SEVİLDİK | Ayşe DİNÇER | Tülin AYDEMİR

Yayıncı:

Muhammet DOĞAN

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Eurasian Journal of Biological and Chemical Sciences 2019 Vol.2 Issue 1, pp.17-23

Belge Tipi:

Makale

Yayın Tarihi:

7/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000421961

eISSN:

2651-5237