Investigation of Biofilm Forming Abilities of Slaughterhouse and Dairy Originated Pathogenic Bacteria by Congo Red Agar and Microplate Methods

Dursun Alp Gündoğ; Güven Güngör; Candan Güngör; Nurhan Ertaş Onmaz; Zafer Gönülalan

Makale detayları

Investigation of Biofilm Forming Abilities of Slaughterhouse and Dairy Originated Pathogenic Bacteria by Congo Red Agar and Microplate Methods

Yazarlar:

Dursun Alp Gündoğ | Güven Güngör | Candan Güngör | Nurhan Ertaş Onmaz | Zafer Gönülalan

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Veteriner Bilimleri

Dergi:

Bozok Veterinary Sciences Vol.4 Issue 1, pp.18-26

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001294871

pISSN:

2757-5179

Anahtar kelimeler:

Özet:

 In this study, a comparison of the efficacy and sensitivity of Congo Red Agar CCA and Microplate MP methods in determining the biofilm-forming abilities of Staphylococcus aureus, Listeria spp, Escherichia coli, and Salmonella spp isolates isolated from slaughterhouses and dairy farms and posing a risk to public health was intended. It was found that 51.1 of 135 isolates were identified as biofilm producers in CCA and 53 in MP. Among the isolates analysed, the CCA methods sensitivity was highest in S. aureus 97, followed by Listeria spp. 59, Salmonella spp 17. and E. coli 13. The specificity rates were 89, 59 and 39 for E. coli, Salmonella spp. and Listeria spp., respectively. However, since all S. aureus isolates gave positive biofilm results in the MP test, the specificity of CCA could not be determined for this isolate. As a result, the sensitivity of the CCA method was higher only for S. aureus among the analysed isolates, while the selectivity of this method was good for other isolates. It is important to accurately determine the biofilm abilities of pathogen strains that may pose a risk to public health in food and food processing environments. Therefore, it was concluded that the complementary application of CCA with the MP method is important for the reliability of the results and the detection of biofilms.

 In this study, a comparison of the efficacy and sensitivity of Congo Red Agar CCA and Microplate MP methods in determining the biofilm-forming abilities of Staphylococcus aureus, Listeria spp, Escherichia coli, and Salmonella spp isolates isolated from slaughterhouses and dairy farms and posing a risk to public health was intended. It was found that 51.1 of 135 isolates were identified as biofilm producers in CCA and 53 in MP. Among the isolates analysed, the CCA methods sensitivity was highest in S. aureus 97, followed by Listeria spp. 59, Salmonella spp 17. and E. coli 13. The specificity rates were 89, 59 and 39 for E. coli, Salmonella spp. and Listeria spp., respectively. However, since all S. aureus isolates gave positive biofilm results in the MP test, the specificity of CCA could not be determined for this isolate. As a result, the sensitivity of the CCA method was higher only for S. aureus among the analysed isolates, while the selectivity of this method was good for other isolates. It is important to accurately determine the biofilm abilities of pathogen strains that may pose a risk to public health in food and food processing environments. Therefore, it was concluded that the complementary application of CCA with the MP method is important for the reliability of the results and the detection of biofilms.

 In this study, a comparison of the efficacy and sensitivity of Congo Red Agar CCA and Microplate MP methods in determining the biofilm-forming abilities of Staphylococcus aureus, Listeria spp, Escherichia coli, and Salmonella spp isolates isolated from slaughterhouses and dairy farms and posing a risk to public health was intended. It was found that 51.1 of 135 isolates were identified as biofilm producers in CCA and 53 in MP. Among the isolates analysed, the CCA methods sensitivity was highest in S. aureus 97, followed by Listeria spp. 59, Salmonella spp 17. and E. coli 13. The specificity rates were 89, 59 and 39 for E. coli, Salmonella spp. and Listeria spp., respectively. However, since all S. aureus isolates gave positive biofilm results in the MP test, the specificity of CCA could not be determined for this isolate. As a result, the sensitivity of the CCA method was higher only for S. aureus among the analysed isolates, while the selectivity of this method was good for other isolates. It is important to accurately determine the biofilm abilities of pathogen strains that may pose a risk to public health in food and food processing environments. Therefore, it was concluded that the complementary application of CCA with the MP method is important for the reliability of the results and the detection of biofilms.

 Bu çalışmada, daha önceki çalışmalarla mezbaha ve mandıralardan izole edilen ve halk sağlığı açısından risk oluşturan önemli patojenlerden Staphylococcus aureus, Listeria spp., Escherichia coli ve Salmonella spp. izolatlarının biyofilm oluşturma yeteneklerinin belirlenmesinde Kongo Kırmızısı Agar KKA ve Mikroplak MP yöntemlerinin etkinliğinin ve duyarlılıklarının karşılaştırılması amaçlandı. Çalışma sonucunda 135 izolatın 51.1i KKAda, 53ü ise MPde biyofilm üreticisi olarak belirlendi. Analiz edilen izolatlar arasında KKA yönteminin duyarlılığının en yüksek olduğu izolat S. aureus idi 97 bunu Listeria spp. 59, Salmonella spp 17. ve E. coli 13 izledi. Listeria spp., Salmonella spp .ve E. coli seçicilik oranları ise sırasıyla 39, 59 ve 89 idi. Ancak S. aureus izolatlarının tamamı MP testinde biyofilm pozitif sonuç verdiği için KKAnın seçiciliği bu izolat için belirlenemedi. Sonuç olarak, KKA yönteminin duyarlılığı analiz edilen izolatlardan sadece S. aureus için yüksekti, diğer izolatlarda ise bu yöntemin seçiciliğinin iyi olduğu görüldü. Gıda ve gıda işleme ortamlarında halk sağlığı için risk oluşturabilen patojen suşlarına ait biyofilm yeteneklerinin doğru tespit edilmesi önem arz etmektedir. Bu nedenle, KKAnın MP yöntemi ile tamamlayıcı bir şekilde uygulanması sonuçların güvenirliği ve biyofilmlerin tespiti için önemli olduğu sonucuna varıldı.

Makale detayları

Investigation of Biofilm Forming Abilities of Slaughterhouse and Dairy Originated Pathogenic Bacteria by Congo Red Agar and Microplate Methods

Yazarlar:

Dursun Alp Gündoğ | Güven Güngör | Candan Güngör | Nurhan Ertaş Onmaz | Zafer Gönülalan

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Veteriner Bilimleri

Dergi:

Bozok Veterinary Sciences Vol.4 Issue 1, pp.18-26

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001294871

pISSN:

2757-5179

Biofilm

Congo Red Agar

Food pathogens

MicroplateBiyofilm

Gıda patojenleri

Kongo Kırmızısı Agar

Mikroplak

Sayı

Makale detayları

Yazarlar: Dursun Alp Gündoğ | Güven Güngör | Candan Güngör | Nurhan Ertaş Onmaz | Zafer Gönülalan

Yayıncı: Yozgat Bozok University

Ana Başlıklar: Veteriner Bilimleri

Dergi: Bozok Veterinary Sciences Vol.4 Issue 1, pp.18-26

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001294871

pISSN: 2757-5179

Sayı

Yazarlar:

Dursun Alp Gündoğ | Güven Güngör | Candan Güngör | Nurhan Ertaş Onmaz | Zafer Gönülalan

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Veteriner Bilimleri

Dergi:

Bozok Veterinary Sciences Vol.4 Issue 1, pp.18-26

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001294871

pISSN:

2757-5179