Comparison of the Protective Effect of Dekspanthenol in a Model of Chemical Cystitis with Chondroitin Sulfate and Hyaluronic Acid Used in Clinical Practice

Caner Ölmez; Mehmet Kutlu Demirkol; Muhammed Seyithanoğlu; Sezen Koçarslan; Faruk Küçükdurmaz; Sefa Resim

Makale detayları

Comparison of the Protective Effect of Dekspanthenol in a Model of Chemical Cystitis with Chondroitin Sulfate and Hyaluronic Acid Used in Clinical Practice

Yazarlar:

Caner Ölmez | Mehmet Kutlu Demirkol | Muhammed Seyithanoğlu | Sezen Koçarslan | Faruk Küçükdurmaz | Sefa Resim

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 2, pp.22-28

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001295732

pISSN:

1303-6610

Anahtar kelimeler:

Özet:

 Objective: Interstitial cystitis IC, also known as bladder pain syndrome BPS, is a type of chronic suprapubic pain that symptoms include feeling the need to urinate right away and needing to urinate often. Dekspanthenol, a derivative of pantothenic acid vitamin B5 is decreased the oxidative stress and inflammation besides that has been indicated posivitive effect of healing wound. In our study, we aim to compare the effects of Dekspanthenol on inflammation and oxidative stress in the bladder to chondroitin sulfate and hyaluronic acid which used routinely intravesical agents treatment of IC. Materials and Methods: Forty Wistar Albino rats were used as a sample and divided five groups which were determined control, chemical cystitis CC, CCDekspanthenol, CCchondroitin sulfate and CChyaluronic acid groups. The chemical cystitis model was obtained with an intravesical instillation of hydrochloric acid 0.2 mL of 0.4 NHCl and in control group rats were applied the only 0.9 NaCl. The CCDekspanthenol group were treated with 2 mL 0.9 NaCl solution containing 500 mgkg Dekspanthenol. The CCchondroitin sulfate group were treated with 2 mL chondroitin sulfate 0.2 and the CChyaluronic acid group were treated with 2 ml hyaluronic acid 0.5. These agents were applied for that three treatment group twice a week for four weeks. Additionally, the 0.9 NaCl installation was performed for the same period to achieve similar stress in CC and control groups. All rats were performed to cystectomy with an abdominal incision and blood sample were taken from their hearts one day after the end of the treatments. Histopathological assessments were done on bladder tissue and malondialdehid MDA, Superoxide Dismutase SOD and prolidase values were defined in blood samples. Results: It was detected that the leukocyte count of the group treating with Dekspanthenol decreased compared to the cystitis group in the histopathological examination of the tissues p0.05. However, this improvement was less than the other treatment groups. There was a significant difference between the tissue MDA values of the control and cystitis groups, but there was no difference between serum MDA, serum prolidase, and serum and tissue SOD values. The serum and tissue MDA and serum prolidase values decreased and serum and tissue SOD values increased in all three treatment groups compared to the cystitis group p0.05 and with this improvement in MDA, SOD and prolidase values of the treatment groups, it was observed that they approached the levels in the control group without cystitis. Conclusions: The Dekspanthenol treatment reduces oxidative stress, increases anti-oxidative effect and suppresses inflammation at tissue level in the interstitial cystitis model. We believe that Dekspanthenol will be used alone or in combination therapy in interstitial cystitis in the future, because of that it effects were similar like the current agents used in the clinic and cost-effective.

 Objective: Interstitial cystitis IC, also known as bladder pain syndrome BPS, is a type of chronic suprapubic pain that symptoms include feeling the need to urinate right away and needing to urinate often. Dekspanthenol, a derivative of pantothenic acid vitamin B5 is decreased the oxidative stress and inflammation besides that has been indicated posivitive effect of healing wound. In our study, we aim to compare the effects of Dekspanthenol on inflammation and oxidative stress in the bladder to chondroitin sulfate and hyaluronic acid which used routinely intravesical agents treatment of IC. Materials and Methods: Forty Wistar Albino rats were used as a sample and divided five groups which were determined control, chemical cystitis CC, CCDekspanthenol, CCchondroitin sulfate and CChyaluronic acid groups. The chemical cystitis model was obtained with an intravesical instillation of hydrochloric acid 0.2 mL of 0.4 NHCl and in control group rats were applied the only 0.9 NaCl. The CCDekspanthenol group were treated with 2 mL 0.9 NaCl solution containing 500 mgkg Dekspanthenol. The CCchondroitin sulfate group were treated with 2 mL chondroitin sulfate 0.2 and the CChyaluronic acid group were treated with 2 ml hyaluronic acid 0.5. These agents were applied for that three treatment group twice a week for four weeks. Additionally, the 0.9 NaCl installation was performed for the same period to achieve similar stress in CC and control groups. All rats were performed to cystectomy with an abdominal incision and blood sample were taken from their hearts one day after the end of the treatments. Histopathological assessments were done on bladder tissue and malondialdehid MDA, Superoxide Dismutase SOD and prolidase values were defined in blood samples. Results: It was detected that the leukocyte count of the group treating with Dekspanthenol decreased compared to the cystitis group in the histopathological examination of the tissues p0.05. However, this improvement was less than the other treatment groups. There was a significant difference between the tissue MDA values of the control and cystitis groups, but there was no difference between serum MDA, serum prolidase, and serum and tissue SOD values. The serum and tissue MDA and serum prolidase values decreased and serum and tissue SOD values increased in all three treatment groups compared to the cystitis group p0.05 and with this improvement in MDA, SOD and prolidase values of the treatment groups, it was observed that they approached the levels in the control group without cystitis. Conclusions: The Dekspanthenol treatment reduces oxidative stress, increases anti-oxidative effect and suppresses inflammation at tissue level in the interstitial cystitis model. We believe that Dekspanthenol will be used alone or in combination therapy in interstitial cystitis in the future, because of that it effects were similar like the current agents used in the clinic and cost-effective.

 Objective: Interstitial cystitis IC, also known as bladder pain syndrome BPS, is a type of chronic suprapubic pain that symptoms include feeling the need to urinate right away and needing to urinate often. Dekspanthenol, a derivative of pantothenic acid vitamin B5 is decreased the oxidative stress and inflammation besides that has been indicated posivitive effect of healing wound. In our study, we aim to compare the effects of Dekspanthenol on inflammation and oxidative stress in the bladder to chondroitin sulfate and hyaluronic acid which used routinely intravesical agents treatment of IC. Materials and Methods: Forty Wistar Albino rats were used as a sample and divided five groups which were determined control, chemical cystitis CC, CCDekspanthenol, CCchondroitin sulfate and CChyaluronic acid groups. The chemical cystitis model was obtained with an intravesical instillation of hydrochloric acid 0.2 mL of 0.4 NHCl and in control group rats were applied the only 0.9 NaCl. The CCDekspanthenol group were treated with 2 mL 0.9 NaCl solution containing 500 mgkg Dekspanthenol. The CCchondroitin sulfate group were treated with 2 mL chondroitin sulfate 0.2 and the CChyaluronic acid group were treated with 2 ml hyaluronic acid 0.5. These agents were applied for that three treatment group twice a week for four weeks. Additionally, the 0.9 NaCl installation was performed for the same period to achieve similar stress in CC and control groups. All rats were performed to cystectomy with an abdominal incision and blood sample were taken from their hearts one day after the end of the treatments. Histopathological assessments were done on bladder tissue and malondialdehid MDA, Superoxide Dismutase SOD and prolidase values were defined in blood samples. Results: It was detected that the leukocyte count of the group treating with Dekspanthenol decreased compared to the cystitis group in the histopathological examination of the tissues p0.05. However, this improvement was less than the other treatment groups. There was a significant difference between the tissue MDA values of the control and cystitis groups, but there was no difference between serum MDA, serum prolidase, and serum and tissue SOD values. The serum and tissue MDA and serum prolidase values decreased and serum and tissue SOD values increased in all three treatment groups compared to the cystitis group p0.05 and with this improvement in MDA, SOD and prolidase values of the treatment groups, it was observed that they approached the levels in the control group without cystitis. Conclusions: The Dekspanthenol treatment reduces oxidative stress, increases anti-oxidative effect and suppresses inflammation at tissue level in the interstitial cystitis model. We believe that Dekspanthenol will be used alone or in combination therapy in interstitial cystitis in the future, because of that it effects were similar like the current agents used in the clinic and cost-effective.

 Amaç: Bir kimyasal sistit hayvan modelinde intravezikal dekspantenol kullanımının mesane histolojisi ve inflamatuvar parametreler üzerindeki etkilerini klinik pratikte kullanılan kondroitin sülfat ve hyaluronik asit ile karşılaştırmak. Gereç ve Yöntemler: Her grupta 8 adet rat olacak şekilde 40 adet Wistar Albino rat 5 gruba ayrıldı. Bu gruplar; kontrol, kimyasal sistit CC, CCdekspantenol, CCkondroitin sülfat ve CChyaluronik asit grupları olarak belirlendi. Deneysel sistit modeli intravezikal hidroklorik asit 0.2 mL of 0.4 NHCl instilasyonu ile sağlandı. Kontrol grubundaki sıçanlara sadece 0.9 NaCl instilasyonu yapıldı. Tedavi gruplarından CCdekspantenol grubuna 500 mgkg Dekspantenol içeren 2 mL 0.9 NaCl solüsyonu, CCkondroitin sülfat grubuna 2 mL kondroitin sülfat 0.2 ve CChyaluronik asit grubuna 2 ml hyaluronik asit0.5 instile edildi. Tedavi gruplarına verilen bu ajanlar dört hafta boyunca haftada iki kez uygulandı. CC ve kontrol gruplarında da benzer stres oluşturmak için aynı süre boyunca 0.9 NaCl instilasyonu yapıldı. Tedavilerin bitiminden bir gün sonra abdominal insizyon ile sistektomi yapıldı. Kalplerinden kan alındıktan sonra ratlar sakrifiye edildi. Mesane dokusu histopatolojik değişiklikler açısından incelendi. Serumda ve dokuda malondialdehit MDA, Süperoksit Dismutaz SOD ve prolidaz değerlerine bakıldı. Bulgular: Dokuların histopatolojik incelenmesinde dekspantenol tedavisi alan grubun lökosit sayısının sistit grubuna göre azaldığı görüldü p0.05. Ancak bu düzelme diğer tedavi gruplarına kıyasla daha düşük idi. Kontrol ve sistit gruplarının doku MDA değerleri arasında anlamlı fark mevcut iken, serum MDA, serum prolidaz ve serum ve doku SOD değerleri arasında farklılık yoktu. Sistit grubuna göre her üç tedavi grubunun serum ve doku MDA ve serum prolidaz değerlerinin azaldığı, serum ve doku SOD değerlerinin arttığı görüldü p0.05. Tedavi gruplarının MDA, SOD ve prolidaz değerlerindeki bu olumlu düzelme ile sistit oluşturulmayan kontrol grubundaki seviyelere yaklaştığı görüldü. Sonuç: İntertisyel sistit modelinde dekspantenolün klinikte kullanılan mevcut ajanlar gibi oksidatif stresi azaltması, anti-oksidatif etkiyi arttırması, doku düzeyindeki enflamasyonu baskılaması ve maliyetinin düşük olması nedeniyle gelecekte intertisyel sistitte tek başına yada kombine tedavide yer bulacağı kanaatindeyiz.

Makale detayları

Comparison of the Protective Effect of Dekspanthenol in a Model of Chemical Cystitis with Chondroitin Sulfate and Hyaluronic Acid Used in Clinical Practice

Yazarlar:

Caner Ölmez | Mehmet Kutlu Demirkol | Muhammed Seyithanoğlu | Sezen Koçarslan | Faruk Küçükdurmaz | Sefa Resim

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 2, pp.22-28

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001295732

pISSN:

1303-6610

chemical cystitis

chondroitin sulfate

dexpanthenol

hyaluronic acid

intravesical treatmentkimyasal sistit

kondroitin sülfat

dekspantenol

hyaluronik asit

intravezikal tedavi

Sayı

Makale detayları

Yazarlar: Caner Ölmez | Mehmet Kutlu Demirkol | Muhammed Seyithanoğlu | Sezen Koçarslan | Faruk Küçükdurmaz | Sefa Resim

Yayıncı: Kahramanmaras Sutcu Imam University

Ana Başlıklar: Sağlık Kurumları Yönetimi

Dergi: Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 2, pp.22-28

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001295732

pISSN: 1303-6610

Sayı

Yazarlar:

Caner Ölmez | Mehmet Kutlu Demirkol | Muhammed Seyithanoğlu | Sezen Koçarslan | Faruk Küçükdurmaz | Sefa Resim

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 2, pp.22-28

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001295732

pISSN:

1303-6610