Investigation of Toxicity Parameters of La-TiO₂ Nanoparticles in Allium cepa L. Root Tip Cells

Aytül Uzun Akgeyik; Emrah Akgeyik

Makale detayları

Investigation of Toxicity Parameters of La-TiO₂ Nanoparticles in Allium cepa L. Root Tip Cells

Yazarlar:

Aytül Uzun Akgeyik | Emrah Akgeyik

Yayıncı:

Erzincan Binali Yildirim University

Ana Başlıklar:

Bitki Hücresi ve Moleküler Biyoloji | Nanokimya

Dergi:

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 1, pp.291-311

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343704

pISSN:

2149-4584

Anahtar kelimeler:

Özet:

 In this study, the dose-dependent physiological, anatomical, and cytogenetic effects of lanthanum-doped titanium dioxide La-TiO₂ nanoparticles, which are widely used today and have applications in electronics, optics, energy storage, and photocatalysis, were investigated in Allium cepa L. root tip cells. La-TiO₂ NPs were synthesized using the microwave method, and characterization analyses such as SEM and XRD were performed to ensure the desired nanoparticle properties before proceeding to in vivo parameters. In the study, physiological parameters including germination percentage, root length, and weight increase; cytogenetic indicators including chromosomal abnormality CA, micronucleus MN frequency, and mitotic index MI; and anatomical effects on meristematic cell damage were examined through sectioning, and the obtained data were statistically evaluated. The onion bulbs used as test materials were divided into five groups: Control Group I and groups Group II 10 ppm, Group III 25 ppm, Group IV 50 ppm and Group V 100 ppm exposed to different doses of La-TiO₂ nanoparticles. After a 72-hour application, it was determined that physiological parameters decreased in groups exposed to La-TiO₂ nanoparticles as the applied dose increased. Additionally, an increase in chromosomal abnormality CA and micronucleus MN frequency was observed. In terms of anatomical damage, epidermal cell damage, thickened cortical cell walls, flattened cell nuclei, and indistinct vascular tissues were detected in groups treated with La-TiO₂. As a result, it was demonstrated that the La-TiO₂ nanoparticles, synthesized in the desired sizes and used in this study, could cause cytotoxic, genotoxic, and anatomical damage in A. cepa L. root tip cells.

 In this study, the dose-dependent physiological, anatomical, and cytogenetic effects of lanthanum-doped titanium dioxide La-TiO₂ nanoparticles, which are widely used today and have applications in electronics, optics, energy storage, and photocatalysis, were investigated in Allium cepa L. root tip cells. La-TiO₂ NPs were synthesized using the microwave method, and characterization analyses such as SEM and XRD were performed to ensure the desired nanoparticle properties before proceeding to in vivo parameters. In the study, physiological parameters including germination percentage, root length, and weight increase; cytogenetic indicators including chromosomal abnormality CA, micronucleus MN frequency, and mitotic index MI; and anatomical effects on meristematic cell damage were examined through sectioning, and the obtained data were statistically evaluated. The onion bulbs used as test materials were divided into five groups: Control Group I and groups Group II 10 ppm, Group III 25 ppm, Group IV 50 ppm and Group V 100 ppm exposed to different doses of La-TiO₂ nanoparticles. After a 72-hour application, it was determined that physiological parameters decreased in groups exposed to La-TiO₂ nanoparticles as the applied dose increased. Additionally, an increase in chromosomal abnormality CA and micronucleus MN frequency was observed. In terms of anatomical damage, epidermal cell damage, thickened cortical cell walls, flattened cell nuclei, and indistinct vascular tissues were detected in groups treated with La-TiO₂. As a result, it was demonstrated that the La-TiO₂ nanoparticles, synthesized in the desired sizes and used in this study, could cause cytotoxic, genotoxic, and anatomical damage in A. cepa L. root tip cells.

 In this study, the dose-dependent physiological, anatomical, and cytogenetic effects of lanthanum-doped titanium dioxide La-TiO₂ nanoparticles, which are widely used today and have applications in electronics, optics, energy storage, and photocatalysis, were investigated in Allium cepa L. root tip cells. La-TiO₂ NPs were synthesized using the microwave method, and characterization analyses such as SEM and XRD were performed to ensure the desired nanoparticle properties before proceeding to in vivo parameters. In the study, physiological parameters including germination percentage, root length, and weight increase; cytogenetic indicators including chromosomal abnormality CA, micronucleus MN frequency, and mitotic index MI; and anatomical effects on meristematic cell damage were examined through sectioning, and the obtained data were statistically evaluated. The onion bulbs used as test materials were divided into five groups: Control Group I and groups Group II 10 ppm, Group III 25 ppm, Group IV 50 ppm and Group V 100 ppm exposed to different doses of La-TiO₂ nanoparticles. After a 72-hour application, it was determined that physiological parameters decreased in groups exposed to La-TiO₂ nanoparticles as the applied dose increased. Additionally, an increase in chromosomal abnormality CA and micronucleus MN frequency was observed. In terms of anatomical damage, epidermal cell damage, thickened cortical cell walls, flattened cell nuclei, and indistinct vascular tissues were detected in groups treated with La-TiO₂. As a result, it was demonstrated that the La-TiO₂ nanoparticles, synthesized in the desired sizes and used in this study, could cause cytotoxic, genotoxic, and anatomical damage in A. cepa L. root tip cells.

 Bu çalışmada günümüzde artık yaygın kullanılan nanopartiküllerden NP biri olan ve elektronik ve optik uygulamalar, enerji depolama ve fotokatalizör olarak da kullanılan Lantan katkılı titantum dioksit La-TiO2 nanopartiküllerinin Allium cepa L. kök ucu hücrelerinde meydana getirdiği doza bağlı fizyolojik, anatomik ve sitogenetik etkileri araştırılmıştır. Mikrodalga yöntemiyle sentezlenen La-TiO2 NPlerin SEM, XRD gibi karakterizasyon analizleri yapılıp istenen özellikte bir nanopartikül sentezinden sonra çalışmadaki in vivo parametrelere geçilmiştir. Çalışmada fizyolojik parametreler açısından: çimlenme yüzdesi, kök uzunluğu ve ağırlık artışı; sitogenetik indikatörler açısından kromozom anormalliği KA, mikronükleus MN sıklığı, mitotik indeks MI; anatomik olarak meristematik hücre hasarları kesitler alınarak incelenmiş ve elde edilen veriler istatistiksel açıdan değerlendirilmiştir. Test materyali olarak kullanılan soğanlar kontrol Grup I ve La-TiO2 naopartikülü ile farklı dozlarlarda uygulanan Grup II 10 ppm, Grup III 25 ppm, Grup IV 50 ppm ve Grup V 100 ppm olmak üzere beş gruba ayrılmıştır. 72 saat yapılan uygulama sonucunda La-TiO2 nanopartiküle maruz bırakılan gruplarda uygulanan doz miktarındaki artışa bağlı olarak fizyolojik parametrelerde azalma meydana geldiği belirlenmiş olup, KA ve MN sıklığı açısından bir artış olduğu tespit edilmiştir. Anatomik hasarlar bakımından epidermal hücre hasarı, kalınlaşmış korteks hücre duvarı, yassılaşmış hücre çekirdekleri ve belirgin olmayan iletim dokusu La-TiO2 uygulanan gruplarda gözlemlenmiştir. Sonuç olarak istenen boyutlarda sentezlenen ve çalışmada kullanılan La-TiO2 NPlerin A.cepa L. kök ucu hücrelerde sitotoksik, genotoksik ve anatomik hasara sebep olan etkiler oluşturabildiği gösterilmiştir.

Makale detayları

Investigation of Toxicity Parameters of La-TiO₂ Nanoparticles in Allium cepa L. Root Tip Cells

Yazarlar:

Aytül Uzun Akgeyik | Emrah Akgeyik

Yayıncı:

Erzincan Binali Yildirim University

Ana Başlıklar:

Bitki Hücresi ve Moleküler Biyoloji | Nanokimya

Dergi:

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 1, pp.291-311

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343704

pISSN:

2149-4584

A.cepa L.

La-TiO2 nanoparticle

micronucleus

cytogenetics

meristematic damageA.cepa L.

La-TiO2 nanopartikül

mikronükleus

sitogenetik

meristematik hasar

Sayı

Makale detayları

Yazarlar: Aytül Uzun Akgeyik | Emrah Akgeyik

Yayıncı: Erzincan Binali Yildirim University

Ana Başlıklar: Bitki Hücresi ve Moleküler Biyoloji | Nanokimya

Dergi: Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 1, pp.291-311

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343704

pISSN: 2149-4584

Sayı

Yazarlar:

Aytül Uzun Akgeyik | Emrah Akgeyik

Yayıncı:

Erzincan Binali Yildirim University

Ana Başlıklar:

Bitki Hücresi ve Moleküler Biyoloji | Nanokimya

Dergi:

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 1, pp.291-311

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343704

pISSN:

2149-4584