Effect of Pulse Width and Intensity on Cell Death in Reversible Electroporation of Cancerous Cells

Mehmet Eşref Alkış; Yusuf Alan; Erhan Eser

Makale detayları

Effect of Pulse Width and Intensity on Cell Death in Reversible Electroporation of Cancerous Cells

Yazarlar:

Mehmet Eşref Alkış | Yusuf Alan | Erhan Eser

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Cancer Biology

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.530-536

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327640

pISSN:

2147-3129

Anahtar kelimeler:

Özet:

 Electroporation EP is the process of increasing the permeability of a biological cell or tissue by applying a short-term and sufficient external electric field. The utilization of proper pulse settings is required for EP-based treatments to be successful. Our aim in this study is to examine the effect of different electrical pulse widths and strength on EP efficiency. Human osteosarcoma cells U20S were used in the study. Eight-square-pulses with a frequency of 1Hz at 10µs, 1ms, 5ms, 10ms, and 20ms widths with low electric fields 20-500Vcm were applied to U20S cells. 10-15 minutes after the applications, the cells were incubated in 96-well plates with 10 thousand cells in each well for 24 hours. Efficiency of pulses of different intensity and width was evaluated by MTT analysis method. The percent inhibition of U20S cancer cells elevated as the pulse width increased in almost all electric field values. The highest cell inhibition  occurred in pulses with an electric field of 500 Vcm and a width of 20ms inhibition ratio: 76.25. No inhibition was observed in the cells at 10µs, 1ms, 5ms, 10ms width pulses with 20 Vcm electric field and 10µs, 1ms width pulses with 50Vcm electric field. In conclusion, our findings show that the electric field intensity and pulse width used in electroporation play an important role in U20S cancer cell death. According to our results, it may be more appropriate to use high-voltage short-width pulses or low-voltage long-width pulses in reversible EP studies.

 Electroporation EP is the process of increasing the permeability of a biological cell or tissue by applying a short-term and sufficient external electric field. The utilization of proper pulse settings is required for EP-based treatments to be successful. Our aim in this study is to examine the effect of different electrical pulse widths and strength on EP efficiency. Human osteosarcoma cells U20S were used in the study. Eight-square-pulses with a frequency of 1Hz at 10µs, 1ms, 5ms, 10ms, and 20ms widths with low electric fields 20-500Vcm were applied to U20S cells. 10-15 minutes after the applications, the cells were incubated in 96-well plates with 10 thousand cells in each well for 24 hours. Efficiency of pulses of different intensity and width was evaluated by MTT analysis method. The percent inhibition of U20S cancer cells elevated as the pulse width increased in almost all electric field values. The highest cell inhibition  occurred in pulses with an electric field of 500 Vcm and a width of 20ms inhibition ratio: 76.25. No inhibition was observed in the cells at 10µs, 1ms, 5ms, 10ms width pulses with 20 Vcm electric field and 10µs, 1ms width pulses with 50Vcm electric field. In conclusion, our findings show that the electric field intensity and pulse width used in electroporation play an important role in U20S cancer cell death. According to our results, it may be more appropriate to use high-voltage short-width pulses or low-voltage long-width pulses in reversible EP studies.

 Electroporation EP is the process of increasing the permeability of a biological cell or tissue by applying a short-term and sufficient external electric field. The utilization of proper pulse settings is required for EP-based treatments to be successful. Our aim in this study is to examine the effect of different electrical pulse widths and strength on EP efficiency. Human osteosarcoma cells U20S were used in the study. Eight-square-pulses with a frequency of 1Hz at 10µs, 1ms, 5ms, 10ms, and 20ms widths with low electric fields 20-500Vcm were applied to U20S cells. 10-15 minutes after the applications, the cells were incubated in 96-well plates with 10 thousand cells in each well for 24 hours. Efficiency of pulses of different intensity and width was evaluated by MTT analysis method. The percent inhibition of U20S cancer cells elevated as the pulse width increased in almost all electric field values. The highest cell inhibition  occurred in pulses with an electric field of 500 Vcm and a width of 20ms inhibition ratio: 76.25. No inhibition was observed in the cells at 10µs, 1ms, 5ms, 10ms width pulses with 20 Vcm electric field and 10µs, 1ms width pulses with 50Vcm electric field. In conclusion, our findings show that the electric field intensity and pulse width used in electroporation play an important role in U20S cancer cell death. According to our results, it may be more appropriate to use high-voltage short-width pulses or low-voltage long-width pulses in reversible EP studies.

 Electroporation EP is the process of increasing the permeability of a biological cell or tissue by applying a short-term and sufficient external electric field. The utilization of proper pulse settings is required for EP-based treatments to be successful. Our aim in this study is to examine the effect of different electrical pulse widths and strength on EP efficiency. Human osteosarcoma cells U20S were used in the study. Eight-square-pulses with a frequency of 1Hz at 10µs, 1ms, 5ms, 10ms, and 20ms widths with low electric fields 20-500Vcm were applied to U20S cells. 10-15 minutes after the applications, the cells were incubated in 96-well plates with 10 thousand cells in each well for 24 hours. Efficiency of pulses of different intensity and width was evaluated by MTT analysis method. The percent inhibition of U20S cancer cells elevated as the pulse width increased in almost all electric field values. The highest cell inhibition  occurred in pulses with an electric field of 500 Vcm and a width of 20ms inhibition ratio: 76.25. No inhibition was observed in the cells at 10µs, 1ms, 5ms, 10ms width pulses with 20 Vcm electric field and 10µs, 1ms width pulses with 50Vcm electric field. In conclusion, our findings show that the electric field intensity and pulse width used in electroporation play an important role in U20S cancer cell death. According to our results, it may be more appropriate to use high-voltage short-width pulses or low-voltage long-width pulses in reversible EP studies.

Makale detayları

Effect of Pulse Width and Intensity on Cell Death in Reversible Electroporation of Cancerous Cells

Yazarlar:

Mehmet Eşref Alkış | Yusuf Alan | Erhan Eser

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Cancer Biology

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.530-536

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327640

pISSN:

2147-3129

Electroporation

Pulse Width

Pulse Strength

Osteosarcoma

Toxicity

Sayı

Makale detayları

Effect of Pulse Width and Intensity on Cell Death in Reversible Electroporation of Cancerous Cells

Yazarlar: Mehmet Eşref Alkış | Yusuf Alan | Erhan Eser

Yayıncı: Bitlis Eren University

Ana Başlıklar: Cancer Biology

Dergi: Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.530-536

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001327640

pISSN: 2147-3129

Electroporation

Pulse Width

Pulse Strength

Osteosarcoma

Toxicity

Sayı

Yazarlar:

Mehmet Eşref Alkış | Yusuf Alan | Erhan Eser

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Cancer Biology

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.530-536

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327640

pISSN:

2147-3129