In Vitro Vitamin C Equivalent Antioxidant Capacity, Cytotoxicity and Anti-Cancer Activity of Methanolic Urtica dioica L. Leaf Extract as a Food Supplement

Serol Korkmaz; Hanife Banu Aydın; İrem Korkmaz

Makale detayları

In Vitro Vitamin C Equivalent Antioxidant Capacity, Cytotoxicity and Anti-Cancer Activity of Methanolic Urtica dioica L. Leaf Extract as a Food Supplement

Yazarlar:

Serol Korkmaz | Hanife Banu Aydın | İrem Korkmaz

Yayıncı:

Selcuk University

Ana Başlıklar:

Tıbbi ve Aromatik Bitkiler

Dergi:

Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 1, pp.140-146

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316275

pISSN:

2458-8377

Anahtar kelimeler:

Özet:

 Antioxidant capacity, cytotoxicity on two vital cell lines and the anti-cancer activity of methanolic Urtica dioica L. leaf extract UDE collected from Duzkoy, Giresun, Turkey were studied by determining safe concentration. The antioxidant capacity of the extract was expressed as vitamin C equivalency by spectrophotometric MTT assay. The cytotoxic concentration 50 was measured by the linearity between UDE concentrations CC50 and the cell viability of non-cancer kidney cell lines BHK-21, MDBK. The anti-cancer activity was conducted on human hepatocellular carcinoma cells HepG2 by determining inhibition concentration IC50 on cell proliferation. The vitamin C equivalence of UDE increased linearly by increasing the concentration. The cytotoxic and non-toxic concentrations of UDE were determined on BHK-21 and MDBK with 15.71 mgml and 5.14 mgml of CC50 respectively. The extract inhibited the proliferation of human hepatocellular carcinoma cells with a 2.46 mgml of IC50. In conclusion, the present study tried to explain in detail the dose-dependent activity of Urtica dioica L. leaf extract. The dose-response results showed that Urtica dioica L. leaf extract could have low cytotoxicity, but potential anti-cancer activity at safe concentrations.

 Antioxidant capacity, cytotoxicity on two vital cell lines and the anti-cancer activity of methanolic Urtica dioica L. leaf extract UDE collected from Duzkoy, Giresun, Turkey were studied by determining safe concentration. The antioxidant capacity of the extract was expressed as vitamin C equivalency by spectrophotometric MTT assay. The cytotoxic concentration 50 was measured by the linearity between UDE concentrations CC50 and the cell viability of non-cancer kidney cell lines BHK-21, MDBK. The anti-cancer activity was conducted on human hepatocellular carcinoma cells HepG2 by determining inhibition concentration IC50 on cell proliferation. The vitamin C equivalence of UDE increased linearly by increasing the concentration. The cytotoxic and non-toxic concentrations of UDE were determined on BHK-21 and MDBK with 15.71 mgml and 5.14 mgml of CC50 respectively. The extract inhibited the proliferation of human hepatocellular carcinoma cells with a 2.46 mgml of IC50. In conclusion, the present study tried to explain in detail the dose-dependent activity of Urtica dioica L. leaf extract. The dose-response results showed that Urtica dioica L. leaf extract could have low cytotoxicity, but potential anti-cancer activity at safe concentrations.

 Antioxidant capacity, cytotoxicity on two vital cell lines and the anti-cancer activity of methanolic Urtica dioica L. leaf extract UDE collected from Duzkoy, Giresun, Turkey were studied by determining safe concentration. The antioxidant capacity of the extract was expressed as vitamin C equivalency by spectrophotometric MTT assay. The cytotoxic concentration 50 was measured by the linearity between UDE concentrations CC50 and the cell viability of non-cancer kidney cell lines BHK-21, MDBK. The anti-cancer activity was conducted on human hepatocellular carcinoma cells HepG2 by determining inhibition concentration IC50 on cell proliferation. The vitamin C equivalence of UDE increased linearly by increasing the concentration. The cytotoxic and non-toxic concentrations of UDE were determined on BHK-21 and MDBK with 15.71 mgml and 5.14 mgml of CC50 respectively. The extract inhibited the proliferation of human hepatocellular carcinoma cells with a 2.46 mgml of IC50. In conclusion, the present study tried to explain in detail the dose-dependent activity of Urtica dioica L. leaf extract. The dose-response results showed that Urtica dioica L. leaf extract could have low cytotoxicity, but potential anti-cancer activity at safe concentrations.

 Antioxidant capacity, cytotoxicity on two vital cell lines and the anti-cancer activity of methanolic Urtica dioica L. leaf extract UDE collected from Duzkoy, Giresun, Turkey were studied by determining safe concentration. The antioxidant capacity of the extract was expressed as vitamin C equivalency by spectrophotometric MTT assay. The cytotoxic concentration 50 was measured by the linearity between UDE concentrations CC50 and the cell viability of non-cancer kidney cell lines BHK-21, MDBK. The anti-cancer activity was conducted on human hepatocellular carcinoma cells HepG2 by determining inhibition concentration IC50 on cell proliferation. The vitamin C equivalence of UDE increased linearly by increasing the concentration. The cytotoxic and non-toxic concentrations of UDE were determined on BHK-21 and MDBK with 15.71 mgml and 5.14 mgml of CC50 respectively. The extract inhibited the proliferation of human hepatocellular carcinoma cells with a 2.46 mgml of IC50. In conclusion, the present study tried to explain in detail the dose-dependent activity of Urtica dioica L. leaf extract. The dose-response results showed that Urtica dioica L. leaf extract could have low cytotoxicity, but potential anti-cancer activity at safe concentrations.

Makale detayları

In Vitro Vitamin C Equivalent Antioxidant Capacity, Cytotoxicity and Anti-Cancer Activity of Methanolic Urtica dioica L. Leaf Extract as a Food Supplement

Yazarlar:

Serol Korkmaz | Hanife Banu Aydın | İrem Korkmaz

Yayıncı:

Selcuk University

Ana Başlıklar:

Tıbbi ve Aromatik Bitkiler

Dergi:

Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 1, pp.140-146

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316275

pISSN:

2458-8377

anti-cancer

cytotoxicity

nettle

Urtica dioica L.

Sayı

Makale detayları

In Vitro Vitamin C Equivalent Antioxidant Capacity, Cytotoxicity and Anti-Cancer Activity of Methanolic Urtica dioica L. Leaf Extract as a Food Supplement

Yazarlar: Serol Korkmaz | Hanife Banu Aydın | İrem Korkmaz

Yayıncı: Selcuk University

Ana Başlıklar: Tıbbi ve Aromatik Bitkiler

Dergi: Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 1, pp.140-146

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001316275

pISSN: 2458-8377

anti-cancer

cytotoxicity

nettle

Urtica dioica L.

Sayı

Yazarlar:

Serol Korkmaz | Hanife Banu Aydın | İrem Korkmaz

Yayıncı:

Selcuk University

Ana Başlıklar:

Tıbbi ve Aromatik Bitkiler

Dergi:

Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 1, pp.140-146

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316275

pISSN:

2458-8377